LOCUS       sequence-250718-       12107 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR L747_T751delLREAT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12107)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-L747_T751delLREAT
FEATURES             Location/Qualifiers
     source          1..12107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    194..311
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        359..1537
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          590..1528
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1485..1505
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1557..1581
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1584..1655,1656..3518,3519..3587,3588..5201)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKESPKANKE
                     ILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNW
                     CVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKV
                     PIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQ
                     PPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTD
                     SNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRN
                     GLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHN
                     QPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPD
                     YQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5203..5227)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5235..5253
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5278..5297)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5279..5297)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5303..5323)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5377..5418
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5439..6006
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5456..6008
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5600..5617)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5827..5846
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6020..6619
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6020..6039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6516..6536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6633..7221
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6686..6706)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7104..7115)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7296..7529
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7648..7666)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7734..7838
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7839..7907,7908..8699)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8870..9458
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9359..9378
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9612..9629
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9869..9890
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9905..9910,9911..9928,9929..9935)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9943..9959
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9948..9970
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9967..9983
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9967..9983
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10033..10052
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10244..10877
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10924..11049
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11546..11779
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11964..12008
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
       61 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
      121 gacagatcca ttcgattagt gaacggatct cgacggtatc gccgaattca caaatggcag
      181 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
      241 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
      301 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggact agtcgtgagg
      361 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      421 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      481 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      541 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
      601 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
      661 ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga
      721 gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc
      781 tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt
      841 cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca
      901 agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc
      961 gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc
     1021 gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg
     1081 cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt
     1141 tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac
     1201 gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc
     1261 ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta
     1321 gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga
     1381 gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt
     1441 ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt
     1501 ggttcaaagt ttttttcttc catttcaggt gtcgtgaagc ggccctgcag atatcaacaa
     1561 gtttgtacaa aaaagcaggc accatgcgac cctccgggac ggccggggca gcgctcctgg
     1621 cgctgctggc tgcgctctgc ccggcgagtc gggctctgga ggaaaagaaa gtttgccaag
     1681 gcacgagtaa caagctcacg cagttgggca cttttgaaga tcattttctc agcctccaga
     1741 ggatgttcaa taactgtgag gtggtccttg ggaatttgga aattacctat gtgcagagga
     1801 attatgatct ttccttctta aagaccatcc aggaggtggc tggttatgtc ctcattgccc
     1861 tcaacacagt ggagcgaatt cctttggaaa acctgcagat catcagagga aatatgtact
     1921 acgaaaattc ctatgcctta gcagtcttat ctaactatga tgcaaataaa accggactga
     1981 aggagctgcc catgagaaat ttacaggaaa tcctgcatgg cgccgtgcgg ttcagcaaca
     2041 accctgccct gtgcaacgtg gagagcatcc agtggcggga catagtcagc agtgactttc
     2101 tcagcaacat gtcgatggac ttccagaacc acctgggcag ctgccaaaag tgtgatccaa
     2161 gctgtcccaa tgggagctgc tggggtgcag gagaggagaa ctgccagaaa ctgaccaaaa
     2221 tcatctgtgc ccagcagtgc tccgggcgct gccgtggcaa gtcccccagt gactgctgcc
     2281 acaaccagtg tgctgcaggc tgcacaggcc cccgggagag cgactgcctg gtctgccgca
     2341 aattccgaga cgaagccacg tgcaaggaca cctgcccccc actcatgctc tacaacccca
     2401 ccacgtacca gatggatgtg aaccccgagg gcaaatacag ctttggtgcc acctgcgtga
     2461 agaagtgtcc ccgtaattat gtggtgacag atcacggctc gtgcgtccga gcctgtgggg
     2521 ccgacagcta tgagatggag gaagacggcg tccgcaagtg taagaagtgc gaagggcctt
     2581 gccgcaaagt gtgtaacgga ataggtattg gtgaatttaa agactcactc tccataaatg
     2641 ctacgaatat taaacacttc aaaaactgca cctccatcag tggcgatctc cacatcctgc
     2701 cggtggcatt taggggtgac tccttcacac atactcctcc tctggatcca caggaactgg
     2761 atattctgaa aaccgtaaag gaaatcacag ggtttttgct gattcaggct tggcctgaaa
     2821 acaggacgga cctccatgcc tttgagaacc tagaaatcat acgcggcagg accaagcaac
     2881 atggtcagtt ttctcttgca gtcgtcagcc tgaacataac atccttggga ttacgctccc
     2941 tcaaggagat aagtgatgga gatgtgataa tttcaggaaa caaaaatttg tgctatgcaa
     3001 atacaataaa ctggaaaaaa ctgtttggga cctccggtca gaaaaccaaa attataagca
     3061 acagaggtga aaacagctgc aaggccacag gccaggtctg ccatgccttg tgctcccccg
     3121 agggctgctg gggcccggag cccagggact gcgtctcttg ccggaatgtc agccgaggca
     3181 gggaatgcgt ggacaagtgc aaccttctgg agggtgagcc aagggagttt gtggagaact
     3241 ctgagtgcat acagtgccac ccagagtgcc tgcctcaggc catgaacatc acctgcacag
     3301 gacggggacc agacaactgt atccagtgtg cccactacat tgacggcccc cactgcgtca
     3361 agacctgccc ggcaggagtc atgggagaaa acaacaccct ggtctggaag tacgcagacg
     3421 ccggccatgt gtgccacctg tgccatccaa actgcaccta cggatgcact gggccaggtc
     3481 ttgaaggctg tccaacgaat gggcctaaga tcccgtccat cgccactggg atggtggggg
     3541 ccctcctctt gctgctggtg gtggccctgg ggatcggcct cttcatgcga aggcgccaca
     3601 tcgttcggaa gcgcacgctg cggaggctgc tgcaggagag ggagcttgtg gagcctctta
     3661 cacccagtgg agaagctccc aaccaagctc tcttgaggat cttgaaggaa actgaattca
     3721 aaaagatcaa agtgctgggc tccggtgcgt tcggcacggt gtataaggga ctctggatcc
     3781 cagaaggtga gaaagttaaa attcccgtcg ctatcaagga atctccgaaa gccaacaagg
     3841 aaatcctcga tgaagcctac gtgatggcca gcgtggacaa cccccacgtg tgccgcctgc
     3901 tgggcatctg cctcacctcc accgtgcagc tcatcacgca gctcatgccc ttcggctgcc
     3961 tcctggacta tgtccgggaa cacaaagaca atattggctc ccagtacctg ctcaactggt
     4021 gtgtgcagat cgcaaagggc atgaactact tggaggaccg tcgcttggtg caccgcgacc
     4081 tggcagccag gaacgtactg gtgaaaacac cgcagcatgt caagatcaca gattttgggc
     4141 tggccaaact gctgggtgcg gaagagaaag aataccatgc agaaggaggc aaagtgccta
     4201 tcaagtggat ggcattggaa tcaattttac acagaatcta tacccaccag agtgatgtct
     4261 ggagctacgg ggtgaccgtt tgggagttga tgacctttgg atccaagcca tatgacggaa
     4321 tccctgccag cgagatctcc tccatcctgg agaaaggaga acgcctccct cagccaccca
     4381 tatgtaccat cgatgtctac atgatcatgg tcaagtgctg gatgatagac gcagatagtc
     4441 gcccaaagtt ccgtgagttg atcatcgaat tctccaaaat ggcccgagac ccccagcgct
     4501 accttgtcat tcagggggat gaaagaatgc atttgccaag tcctacagac tccaacttct
     4561 accgtgccct gatggatgaa gaagacatgg acgacgtggt ggatgccgac gagtacctca
     4621 tcccacagca gggcttcttc agcagcccct ccacgtcacg gactcccctc ctgagctctc
     4681 tgagtgcaac cagcaacaat tccaccgtgg cttgcattga tagaaatggg ctgcaaagct
     4741 gtcccatcaa ggaagacagc ttcttgcagc gatacagctc agaccccaca ggcgccttga
     4801 ctgaggacag catagacgac accttcctcc cagtgcctga atacataaac cagtccgttc
     4861 ccaaaaggcc cgctggctct gtgcagaatc ctgtctatca caatcagcct ctgaaccccg
     4921 cgcccagcag agacccacac taccaggacc cccacagcac tgcagtgggc aaccccgagt
     4981 atctcaacac tgtccagccc acctgtgtca acagcacatt cgacagccct gcccactggg
     5041 cccagaaagg cagccaccaa attagcctgg acaaccctga ctaccagcag gacttctttc
     5101 ccaaggaagc caagccaaat ggcatcttta agggctccac agctgaaaat gcagaatacc
     5161 taagggtcgc gccacaaagc agtgaattta ttggagcata gaacccagct ttcttgtaca
     5221 aagtggtgat atccaattaa ccctcactaa aggatgaaag atttagttgt gagatatccc
     5281 tatagtgagt cgtattacca cccaactttt ctatacaaag tggttgatat ccagcacagt
     5341 ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc
     5401 ctcggtctcg attctacgcg taccggttag taatgagatc cctccccccc ccctaacgtt
     5461 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     5521 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     5581 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     5641 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     5701 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     5761 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     5821 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     5881 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     5941 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     6001 tgataatatg gccacacata tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     6061 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     6121 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     6181 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     6241 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     6301 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     6361 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     6421 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     6481 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     6541 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     6601 ccgcaagccc ggtgcctaaa tcgatagatc ctaatcaacc tctggattac aaaatttgtg
     6661 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     6721 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     6781 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     6841 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     6901 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     6961 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     7021 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     7081 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     7141 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     7201 ccctttgggc cgcctccccg cctgagatcc tttaagacca atgacttaca aggcagctgt
     7261 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     7321 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     7381 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7441 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7501 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     7561 tttataactt gcaaagaaat gaatatcaga gagtgagagg cccgggttaa ttaaggaaag
     7621 ggctagatca ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     7681 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     7741 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7801 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7861 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7921 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7981 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8041 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     8101 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8161 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8221 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8281 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8341 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     8401 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8461 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8521 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8581 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8641 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8701 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8761 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8821 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8881 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8941 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     9001 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9061 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9121 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9181 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9241 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9301 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9361 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9421 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9481 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9541 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9601 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     9661 ctctccccgc gcgttggccg attcattaat gcagcaagct catggctgac taattttttt
     9721 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     9781 cttttttgga ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg tttcccgact
     9841 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     9901 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     9961 ttcacacagg aaacagctat gacatgatta cgaatttcac aaataaagca tttttttcac
    10021 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
    10081 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
    10141 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    10201 aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac
    10261 tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
    10321 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
    10381 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
    10441 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
    10501 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
    10561 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
    10621 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
    10681 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
    10741 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
    10801 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
    10861 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
    10921 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
    10981 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
    11041 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
    11101 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
    11161 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
    11221 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
    11281 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
    11341 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga
    11401 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
    11461 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
    11521 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
    11581 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
    11641 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
    11701 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
    11761 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
    11821 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
    11881 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
    11941 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
    12001 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
    12061 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactt
//
