LOCUS       sequence-250721-       10924 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of FGFR4 M708I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10924)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-FGFR4-M708I
FEATURES             Location/Qualifiers
     source          1..10924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    20..44
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1218..1229
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(2460..2484)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2537..2578
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2599..3166
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2616..3168
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2760..2777)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2987..3006
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3180..3182,3183..3185,3186..3899)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3180..3182,3183..3185,3186..3896)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3225..3246)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3486..3505)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3833..3854
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3913..4501
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3966..3986)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4384..4395)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4576..4809
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4928..4946)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5014..5118
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5119..5187,5188..5979)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5337..5356)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6150..6738
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6639..6658
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6892..6909
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7149..7170
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7185..7190,7191..7208,7209..7215)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7223..7239
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7228..7250
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7247..7263
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7247..7263
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7313..7332
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7524..8157
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8204..8329
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8826..9059
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9244..9288
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9581..9698
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9746..10924
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9977..10915
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     10872..10892
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 agcggccctg cagatatcaa caagtttgta caaaaaagca ggctccacca tgcggctgct
       61 gctggccctg ttgggggtcc tgctgagtgt gcctgggcct ccagtcttgt ccctggaggc
      121 ctctgaggaa gtggagcttg agccctgcct ggctcccagc ctggagcagc aagagcagga
      181 gctgacagta gcccttgggc agcctgtgcg tctgtgctgt gggcgggctg agcgtggtgg
      241 ccactggtac aaggagggca gtcgcctggc acctgctggc cgtgtacggg gctggagggg
      301 ccgcctagag attgccagct tcctacctga ggatgctggc cgctacctct gcctggcacg
      361 aggctccatg atcgtcctgc agaatctcac cttgattaca ggtgactcct tgacctccag
      421 caacgatgat gaggacccca agtcccatag ggacccctcg aataggcaca gttaccccca
      481 gcaagcaccc tactggacac acccccagcg catggagaag aaactgcatg cagtacctgc
      541 ggggaacacc gtcaagttcc gctgtccagc tgcaggcaac cccacgccca ccatccgctg
      601 gcttaaggat ggacaggcct ttcatgggga gaaccgcatt ggaggcattc ggctgcgcca
      661 tcagcactgg agtctcgtga tggagagcgt ggtgccctcg gaccgcggca catacacctg
      721 cctggtagag aacgctgtgg gcagcatccg ctataactac ctgctagatg tgctggagcg
      781 gtccccgcac cggcccatcc tgcaggccgg gctcccggcc aacaccacag ccgtggtggg
      841 cagcgacgtg gagctgctgt gcaaggtgta cagcgatgcc cagccccaca tccagtggct
      901 gaagcacatc gtcatcaacg gcagcagctt cggagccgac ggtttcccct atgtgcaagt
      961 cctaaagact gcagacatca atagctcaga ggtggaggtc ctgtacctgc ggaacgtgtc
     1021 agccgaggac gcaggcgagt acacctgcct cgcaggcaat tccatcggcc tctcctacca
     1081 gtctgcctgg ctcacggtgc tgccagagga ggaccccaca tggaccgcag cagcgcccga
     1141 ggccaggtat acggacatca tcctgtacgc gtcgggctcc ctggccttgg ctgtgctcct
     1201 gctgctggcc gggctgtatc gagggcaggc gctccacggc cggcaccccc gcccgcccgc
     1261 cactgtgcag aagctctccc gcttccctct ggcccgacag ttctccctgg agtcaggctc
     1321 ttccggcaag tcaagctcat ccctggtacg aggcgtgcgt ctctcctcca gcggccccgc
     1381 cttgctcgcc ggcctcgtga gtctagatct acctctcgac ccactatggg agttcccccg
     1441 ggacaggctg gtgcttggga agcccctagg cgagggctgc tttggccagg tagtacgtgc
     1501 agaggccttt ggcatggacc ctgcccggcc tgaccaagcc agcactgtgg ccgtcaagat
     1561 gctcaaagac aacgcctctg acaaggacct ggccgacctg gtctcggaga tggaggtgat
     1621 gaagctgatc ggccgacaca agaacatcat caacctgctt ggtgtctgca cccaggaagg
     1681 gcccctgtac gtgatcgtgg agtgcgccgc caagggaaac ctgcgggagt tcctgcgggc
     1741 ccggcgcccc ccaggccccg acctcagccc cgacggtcct cggagcagtg aggggccgct
     1801 ctccttccca gtcctggtct cctgcgccta ccaggtggcc cgaggcatgc agtatctgga
     1861 gtcccggaag tgtatccacc gggacctggc tgcccgcaat gtgctggtga ctgaggacaa
     1921 tgtgatgaag attgctgact ttgggctggc ccgcggcgtc caccacattg actactataa
     1981 gaaaaccagc aacggccgcc tgcctgtgaa gtggatggcg cccgaggcct tgtttgaccg
     2041 ggtgtacaca caccagagtg acgtgtggtc ttttgggatc ctgctatggg agatcttcac
     2101 cctcgggggc tccccgtatc ctggcatccc ggtggaggag ctgttctcgc tgctgcggga
     2161 gggacatcgg atagaccgac ccccacactg ccccccagag ctgtacgggc tgatgcgtga
     2221 gtgctggcac gcagcgccct cccagaggcc taccttcaag cagctggtgg aggcgctgga
     2281 caaggtcctg ctggccgtct ctgaggagta cctcgacctc cgcctgacct tcggacccta
     2341 ttccccctct ggtggggacg ccagcagcac ctgctcctcc agcgattctg tcttcagcca
     2401 cgaccccctg ccattgggat ccagctcctt ccccttcggg tctggggtgc agacatagga
     2461 cccagctttc ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgagtctaga
     2521 gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg
     2581 taccggttag taatgagatc cctccccccc ccctaacgtt actggccgaa gccgcttgga
     2641 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     2701 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     2761 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     2821 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     2881 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     2941 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     3001 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     3061 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc
     3121 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacacata
     3181 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     3241 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     3301 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     3361 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     3421 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     3481 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     3541 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     3601 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     3661 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     3721 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     3781 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     3841 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa
     3901 tcgatagatc ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     3961 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     4021 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     4081 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     4141 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     4201 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     4261 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     4321 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     4381 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     4441 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     4501 cctgagatcc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     4561 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     4621 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     4681 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     4741 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     4801 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     4861 gaatatcaga gagtgagagg cccgggttaa ttaaggaaag ggctagatca ttcttgaaga
     4921 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     4981 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5041 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5101 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5161 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5221 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5281 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5341 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     5401 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5461 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5521 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5581 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5641 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     5701 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     5761 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     5821 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     5881 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     5941 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6001 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6061 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6121 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6181 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6241 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6301 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6361 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6421 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6481 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6541 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6601 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6661 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6721 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6781 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6841 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     6901 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     6961 attcattaat gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg
     7021 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     7081 tttgcaaaaa gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     7141 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     7201 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     7261 gacatgatta cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7321 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
     7381 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
     7441 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
     7501 atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat
     7561 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
     7621 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     7681 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
     7741 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
     7801 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
     7861 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
     7921 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
     7981 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     8041 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     8101 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     8161 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     8221 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     8281 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     8341 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     8401 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     8461 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     8521 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     8581 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     8641 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga
     8701 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     8761 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     8821 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     8881 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     8941 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     9001 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     9061 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     9121 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     9181 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     9241 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     9301 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     9361 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     9421 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     9481 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     9541 cggtatcgcc gaattcacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     9601 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     9661 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     9721 gagatccagt ttggactagt cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc
     9781 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
     9841 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     9901 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     9961 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg
    10021 ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc
    10081 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
    10141 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
    10201 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
    10261 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca
    10321 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac
    10381 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca
    10441 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc
    10501 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc
    10561 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc
    10621 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta
    10681 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg
    10741 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt
    10801 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc
    10861 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc
    10921 gtga
//
