LOCUS       sequence-250722-       11057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of DDR2 R473H.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11057)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-DDR2-R473H
FEATURES             Location/Qualifiers
     source          1..11057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    292..409
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        457..1635
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          688..1626
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1583..1603
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1655..1679
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             2578..2586
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(4251..4275)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4283..4301
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4326..4345)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4327..4345)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(4351..4371)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             4425..4466
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4487..5054
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4504..5056
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4648..4665)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4875..4894
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5068..5667
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5068..5087)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5564..5584
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5681..6269
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5734..5754)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6152..6163)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6344..6577
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6696..6714)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6782..6886
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6887..6955,6956..7747)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7105..7124)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7918..8506
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8407..8426
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8660..8677
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8917..8938
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8953..8958,8959..8976,8977..8983)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8991..9007
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8996..9018
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9015..9031
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9015..9031
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9081..9100
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9292..9925
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9972..10097
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10594..10827
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11012..11056
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
       61 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
      121 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
      181 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
      241 acggatctcg acggtatcgc cgaattcaca aatggcagta ttcatccaca attttaaaag
      301 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
      361 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
      421 cagggacagc agagatccag tttggactag tcgtgaggct ccggtgcccg tcagtgggca
      481 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt
      541 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
      601 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
      661 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
      721 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg
      781 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
      841 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
      901 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
      961 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
     1021 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
     1081 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
     1141 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
     1201 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
     1261 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
     1321 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
     1381 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
     1441 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
     1501 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
     1561 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
     1621 tttcaggtgt cgtgaagcgg ccctgcagat atcaacaagt ttgtacaaaa aagcaggcac
     1681 catgatcctg attcccagaa tgctcttggt gctgttcctg ctgctgccta tcttgagttc
     1741 tgcaaaagct caggttaatc cagctatatg ccgctatcct ctgggcatgt caggaggcca
     1801 gattccagat gaggacatca cagcttccag tcagtggtca gagtccacag ctgccaaata
     1861 tggaaggctg gactcagaag aaggggatgg agcctggtgc cctgagattc cagtggaacc
     1921 tgatgacctg aaggagtttc tgcagattga cttgcacacc ctccatttta tcactctggt
     1981 ggggacccag gggcgccatg caggaggtca tggcatcgag tttgccccca tgtacaagat
     2041 caattacagt cgggatggca ctcgctggat ctcttggcgg aaccgtcatg ggaaacaggt
     2101 gctggatgga aatagtaacc cctatgacat tttcctaaag gacttggagc cgcccattgt
     2161 agccagattt gtccggttca ttccagtcac cgaccactcc atgaatgtgt gtatgagagt
     2221 ggagctttac ggctgtgtct ggctagatgg cttggtgtct tacaatgctc cagctgggca
     2281 gcagtttgta ctccctggag gttccatcat ttatctgaat gattctgtct atgatggagc
     2341 tgttggatac agcatgacag aagggctagg ccaattgacc gatggtgtgt ctggcctgga
     2401 cgatttcacc cagacccacg aataccacgt gtggcccggc tatgactatg tgggctggcg
     2461 gaacgagagt gccaccaatg gctacattga gatcatgttt gaatttgacc gcatcaggaa
     2521 tttcactacc atgaaggtcc actgcaacaa catgtttgct aaaggtgtga agatctttaa
     2581 ggaggtacag tgctacttcc gctctgaagc cagtgagtgg gaacctaatg ccatttcctt
     2641 cccccttgtc ctggatgacg tcaaccccag tgctcggttt gtcacggtgc ctctccacca
     2701 ccgaatggcc agtgccatca agtgtcaata ccattttgca gatacctgga tgatgttcag
     2761 tgagatcacc ttccaatcag atgctgcaat gtacaacaac tctgaagccc tgcccacctc
     2821 tcctatggca cccacaacct atgatccaat gcttaaagtt gatgacagca acactcggat
     2881 cctgattggc tgcttggtgg ccatcatctt tatcctcctg gccatcattg tcatcatcct
     2941 ctggaggcag ttctggcaga aaatgctgga gaaggcttct cggaggatgc tggatgatga
     3001 aatgacagtc agcctttccc tgccaagtga ttctagcatg ttcaacaata accgctcctc
     3061 atcacctagt gaacaagggt ccaactcgac ttacgatcac atctttcccc ttcgccctga
     3121 ctaccaggag ccatccaggc tgatacgaaa actcccagaa tttgctccag gggaggagga
     3181 gtcaggctgc agcggtgttg tgaagccagt ccagcccagt ggccctgagg gggtgcccca
     3241 ctatgcagag gctgacatag tgaacctcca aggagtgaca ggaggcaaca catactcagt
     3301 gcctgccgtc accatggacc tgctctcagg aaaagatgtg gctgtggagg agttccccag
     3361 gaaactccta actttcaaag agaagctggg agaaggacag tttggggagg ttcatctctg
     3421 tgaagtggag ggaatggaaa aattcaaaga caaagatttt gccctagatg tcagtgccaa
     3481 ccagcctgtc ctggtggctg tgaaaatgct ccgagcagat gccaacaaga atgccaggaa
     3541 tgattttctt aaggagataa agatcatgtc tcggctcaag gacccaaaca tcatccatct
     3601 attagctgtg tgtatcactg atgaccctct ctgtatgatc actgaataca tggagaatgg
     3661 agatctcaat cagtttcttt cccgccacga gccccctaat tcttcctcca gcgatgtacg
     3721 cactgtcagt tacaccaatc tgaagtttat ggctacccaa attgcctctg gcatgaagta
     3781 cctttcctct cttaattttg ttcaccgaga tctggccaca cgaaactgtt tagtgggtaa
     3841 gaactacaca atcaagatag ctgactttgg aatgagcagg aacctgtaca gtggtgacta
     3901 ttaccggatc cagggccggg cagtgctccc tatccgctgg atgtcttggg agagtatctt
     3961 gctgggcaag ttcactacag caagtgatgt gtgggccttt ggggttactt tgtgggagac
     4021 tttcaccttt tgtcaagaac agccctattc ccagctgtca gatgaacagg ttattgagaa
     4081 tactggagag ttcttccgag accaagggag gcagacttac ctccctcaac cagccatttg
     4141 tcctgactct gtgtataagc tgatgctcag ctgctggaga agagatacga agaaccgtcc
     4201 ctcattccaa gaaatccacc ttctgctcct tcaacaaggc gacgagtaga acccagcttt
     4261 cttgtacaaa gtggtgatat ccaattaacc ctcactaaag gtagataatg tgaagtaata
     4321 agtgacccta tagtgagtcg tattaccacc caacttttct atacaaagtg gttgatatcc
     4381 agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta
     4441 accctctcct cggtctcgat tctacgcgta ccggttagta atgagatccc tccccccccc
     4501 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     4561 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     4621 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     4681 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     4741 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     4801 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     4861 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     4921 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     4981 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     5041 aaacacgatg ataatatggc cacacatatg accgagtaca agcccacggt gcgcctcgcc
     5101 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     5161 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     5221 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     5281 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     5341 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     5401 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     5461 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     5521 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     5581 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     5641 tgcatgaccc gcaagcccgg tgcctaaatc gatagatcct aatcaacctc tggattacaa
     5701 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     5761 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     5821 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     5881 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     5941 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     6001 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     6061 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     6121 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     6181 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     6241 tcggatctcc ctttgggccg cctccccgcc tgagatcctt taagaccaat gacttacaag
     6301 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     6361 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     6421 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6481 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6541 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     6601 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc cgggttaatt
     6661 aaggaaaggg ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     6721 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     6781 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6841 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6901 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6961 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7021 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7081 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     7141 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7201 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7261 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7321 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7381 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7441 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7501 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7561 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7621 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7681 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7741 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7801 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7861 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7921 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7981 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8041 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8101 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8161 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8221 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8281 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8341 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8401 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8461 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8521 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8581 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8641 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     8701 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta
     8761 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     8821 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt
     8881 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     8941 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     9001 ataacaattt cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt
     9061 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9121 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
     9181 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
     9241 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc
     9301 taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     9361 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
     9421 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
     9481 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     9541 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
     9601 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact
     9661 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat
     9721 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     9781 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     9841 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     9901 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     9961 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
    10021 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
    10081 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
    10141 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
    10201 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
    10261 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
    10321 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
    10381 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
    10441 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
    10501 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
    10561 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
    10621 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
    10681 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
    10741 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
    10801 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
    10861 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
    10921 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
    10981 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
    11041 taaatgggca agtttgt
//
