LOCUS       sequence-250724-       10921 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of FGFR4 P788L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10921)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-FGFR4-P788L
FEATURES             Location/Qualifiers
     source          1..10921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..118
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        166..1344
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          397..1335
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1292..1312
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1364..1388
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2559..2570
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(3801..3825)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3878..3919
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3940..4507
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3957..4509
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4101..4118)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4328..4347
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4521..4523,4524..4526,4527..5240)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4521..4523,4524..4526,4527..5237)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4566..4587)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4827..4846)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5174..5195
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5254..5842
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5307..5327)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5725..5736)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5917..6150
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6269..6287)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6355..6459
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6460..6528,6529..7320)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6678..6697)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7491..8079
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7980..7999
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8233..8250
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8490..8511
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8526..8531,8532..8549,8550..8556)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8564..8580
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8569..8591
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8588..8604
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8588..8604
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8654..8673
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8865..9498
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9545..9670
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10167..10400
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10585..10629
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
       61 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      121 ggtttattac agggacagca gagatccagt ttggactagt cgtgaggctc cggtgcccgt
      181 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
      241 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
      301 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
      361 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
      421 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
      481 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
      541 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
      601 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
      661 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
      721 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
      781 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
      841 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
      901 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
      961 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     1021 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     1081 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     1141 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     1201 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     1261 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     1321 tttcttccat ttcaggtgtc gtgaagcggc cctgcagata tcaacaagtt tgtacaaaaa
     1381 agcaggcacc atgcggctgc tgctggccct gttgggggtc ctgctgagtg tgcctgggcc
     1441 tccagtcttg tccctggagg cctctgagga agtggagctt gagccctgcc tggctcccag
     1501 cctggagcag caagagcagg agctgacagt agcccttggg cagcctgtgc gtctgtgctg
     1561 tgggcgggct gagcgtggtg gccactggta caaggagggc agtcgcctgg cacctgctgg
     1621 ccgtgtacgg ggctggaggg gccgcctaga gattgccagc ttcctacctg aggatgctgg
     1681 ccgctacctc tgcctggcac gaggctccat gatcgtcctg cagaatctca ccttgattac
     1741 aggtgactcc ttgacctcca gcaacgatga tgaggacccc aagtcccata gggacccctc
     1801 gaataggcac agttaccccc agcaagcacc ctactggaca cacccccagc gcatggagaa
     1861 gaaactgcat gcagtacctg cggggaacac cgtcaagttc cgctgtccag ctgcaggcaa
     1921 ccccacgccc accatccgct ggcttaagga tggacaggcc tttcatgggg agaaccgcat
     1981 tggaggcatt cggctgcgcc atcagcactg gagtctcgtg atggagagcg tggtgccctc
     2041 ggaccgcggc acatacacct gcctggtaga gaacgctgtg ggcagcatcc gctataacta
     2101 cctgctagat gtgctggagc ggtccccgca ccggcccatc ctgcaggccg ggctcccggc
     2161 caacaccaca gccgtggtgg gcagcgacgt ggagctgctg tgcaaggtgt acagcgatgc
     2221 ccagccccac atccagtggc tgaagcacat cgtcatcaac ggcagcagct tcggagccga
     2281 cggtttcccc tatgtgcaag tcctaaagac tgcagacatc aatagctcag aggtggaggt
     2341 cctgtacctg cggaacgtgt cagccgagga cgcaggcgag tacacctgcc tcgcaggcaa
     2401 ttccatcggc ctctcctacc agtctgcctg gctcacggtg ctgccagagg aggaccccac
     2461 atggaccgca gcagcgcccg aggccaggta tacggacatc atcctgtacg cgtcgggctc
     2521 cctggccttg gctgtgctcc tgctgctggc cgggctgtat cgagggcagg cgctccacgg
     2581 ccggcacccc cgcccgcccg ccactgtgca gaagctctcc cgcttccctc tggcccgaca
     2641 gttctccctg gagtcaggct cttccggcaa gtcaagctca tccctggtac gaggcgtgcg
     2701 tctctcctcc agcggccccg ccttgctcgc cggcctcgtg agtctagatc tacctctcga
     2761 cccactatgg gagttccccc gggacaggct ggtgcttggg aagcccctag gcgagggctg
     2821 ctttggccag gtagtacgtg cagaggcctt tggcatggac cctgcccggc ctgaccaagc
     2881 cagcactgtg gccgtcaaga tgctcaaaga caacgcctct gacaaggacc tggccgacct
     2941 ggtctcggag atggaggtga tgaagctgat cggccgacac aagaacatca tcaacctgct
     3001 tggtgtctgc acccaggaag ggcccctgta cgtgatcgtg gagtgcgccg ccaagggaaa
     3061 cctgcgggag ttcctgcggg cccggcgccc cccaggcccc gacctcagcc ccgacggtcc
     3121 tcggagcagt gaggggccgc tctccttccc agtcctggtc tcctgcgcct accaggtggc
     3181 ccgaggcatg cagtatctgg agtcccggaa gtgtatccac cgggacctgg ctgcccgcaa
     3241 tgtgctggtg actgaggaca atgtgatgaa gattgctgac tttgggctgg cccgcggcgt
     3301 ccaccacatt gactactata agaaaaccag caacggccgc ctgcctgtga agtggatggc
     3361 gcccgaggcc ttgtttgacc gggtgtacac acaccagagt gacgtgtggt cttttgggat
     3421 cctgctatgg gagatcttca ccctcggggg ctccccgtat cctggcatcc cggtggagga
     3481 gctgttctcg ctgctgcggg agggacatcg gatggaccga cccccacact gccccccaga
     3541 gctgtacggg ctgatgcgtg agtgctggca cgcagcgccc tcccagaggc ctaccttcaa
     3601 gcagctggtg gaggcgctgg acaaggtcct gctggccgtc tctgaggagt acctcgacct
     3661 ccgcctgacc ttcggaccct attccccctc tggtggggac gccagcagca cctgctcctc
     3721 cagcgattct gtcttcagcc acgaccccct gctattggga tccagctcct tccccttcgg
     3781 gtctggggtg cagacataga acccagcttt cttgtacaaa gtggttgata tccagcacag
     3841 tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct
     3901 cctcggtctc gattctacgc gtaccggtta gtaatgagat ccctcccccc cccctaacgt
     3961 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     4021 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     4081 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     4141 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     4201 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     4261 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     4321 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     4381 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     4441 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     4501 atgataatat ggccacacat atggtgagca agggcgagga gctgttcacc ggggtggtgc
     4561 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     4621 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     4681 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     4741 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     4801 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     4861 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     4921 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     4981 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     5041 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     5101 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     5161 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     5221 tggacgagct gtacaagtaa atcgatagat cctaatcaac ctctggatta caaaatttgt
     5281 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     5341 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     5401 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     5461 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     5521 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     5581 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     5641 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     5701 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     5761 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     5821 tccctttggg ccgcctcccc gcctgagatc ctttaagacc aatgacttac aaggcagctg
     5881 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     5941 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     6001 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6061 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6121 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     6181 atttataact tgcaaagaaa tgaatatcag agagtgagag gcccgggtta attaaggaaa
     6241 gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     6301 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     6361 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     6421 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     6481 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     6541 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     6601 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     6661 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     6721 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     6781 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6841 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6901 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6961 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     7021 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     7081 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     7141 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     7201 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     7261 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     7321 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     7381 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     7441 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7501 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7561 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7621 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     7681 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7741 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7801 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7861 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7921 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7981 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     8041 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     8101 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     8161 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     8221 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     8281 cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt
     8341 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     8401 gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac
     8461 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     8521 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     8581 tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc atttttttca
     8641 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
     8701 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
     8761 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
     8821 taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca
     8881 ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     8941 tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg
     9001 ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac
     9061 cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga
     9121 gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta
     9181 cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg
     9241 gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc
     9301 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     9361 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     9421 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     9481 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     9541 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     9601 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     9661 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     9721 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     9781 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     9841 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     9901 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     9961 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg
    10021 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
    10081 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
    10141 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
    10201 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
    10261 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
    10321 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
    10381 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
    10441 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
    10501 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
    10561 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
    10621 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
    10681 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
    10741 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
    10801 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
    10861 cgattagtga acggatctcg acggtatcgc cgaattcaca aatggcagta ttcatccaca
    10921 a
//
