LOCUS       sequence-250730-       12507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of FLT1 R781Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12507)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-FLT1-R781Q
FEATURES             Location/Qualifiers
     source          1..12507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    20..44
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4065..4089)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4097..4115
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4140..4159)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4141..4159)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(4165..4185)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             4239..4280
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4301..4869
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4319..4871
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4463..4480)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4690..4709
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4883..5482
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4883..4902)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5379..5399
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5496..6084
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5549..5569)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5967..5978)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6159..6392
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6511..6529)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6597..6701
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6702..6770,6771..7562)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6920..6939)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7733..8321
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8222..8241
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8475..8492
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8732..8753
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8768..8773,8774..8791,8792..8798)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8806..8822
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8811..8833
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8830..8846
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8830..8846
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8896..8915
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9107..9740
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9787..9912
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10409..10642
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10827..10871
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11164..11281
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        11329..12507
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          11560..12498
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     12455..12475
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 agcggccctg cagatatcaa caagtttgta caaaaaagca ggcaccatgg tcagctactg
       61 ggacaccggg gtcctgctgt gcgcgctgct cagctgtctg cttctcacag gatctagttc
      121 aggttcaaaa ttaaaagatc ctgaactgag tttaaaaggc acccagcaca tcatgcaagc
      181 aggccagaca ctgcatctcc aatgcagggg ggaagcagcc cataaatggt ctttgcctga
      241 aatggtgagt aaggaaagcg aaaggctgag cataactaaa tctgcctgtg gaagaaatgg
      301 caaacaattc tgcagtactt taaccttgaa cacagctcaa gcaaaccaca ctggcttcta
      361 cagctgcaaa tatctagctg tacctacttc aaagaagaag gaaacagaat ctgcaatcta
      421 tatatttatt agtgatacag gtagaccttt cgtagagatg tacagtgaaa tccccgaaat
      481 tatacacatg actgaaggaa gggagctcgt cattccctgc cgggttacgt cacctaacat
      541 cactgttact ttaaaaaagt ttccacttga cactttgatc cctgatggaa aacgcataat
      601 ctgggacagt agaaagggct tcatcatatc aaatgcaacg tacaaagaaa tagggcttct
      661 gacctgtgaa gcaacagtca atgggcattt gtataagaca aactatctca cacatcgaca
      721 aaccaataca atcatagatg tccaaataag cacaccacgc ccagtcaaat tacttagagg
      781 ccatactctt gtcctcaatt gtactgctac cactcccttg aacacgagag ttcaaatgac
      841 ctggagttac cctgatgaaa aaaataagag agcttccgta aggcgacgaa ttgaccaaag
      901 caattcccat gccaacatat tctacagtgt tcttactatt gacaaaatgc agaacaaaga
      961 caaaggactt tatacttgtc gtgtaaggag tggaccatca ttcaaatctg ttaacacctc
     1021 agtgcatata tatgataaag cattcatcac tgtgaaacat cgaaaacagc aggtgcttga
     1081 aaccgtagct ggcaagcggt cttaccggct ctctatgaaa gtgaaggcat ttccctcgcc
     1141 ggaagttgta tggttaaaag atgggttacc tgcgactgag aaatctgctc gctatttgac
     1201 tcgtggctac tcgttaatta tcaaggacgt aactgaagag gatgcaggga attatacaat
     1261 cttgctgagc ataaaacagt caaatgtgtt taaaaacctc actgccactc taattgtcaa
     1321 tgtgaaaccc cagatttacg aaaaggccgt gtcatcgttt ccagacccgg ctctctaccc
     1381 actgggcagc agacaaatcc tgacttgtac cgcatatggt atccctcaac ctacaatcaa
     1441 gtggttctgg cacccctgta accataatca ttccgaagca aggtgtgact tttgttccaa
     1501 taatgaagag tcctttatcc tggatgctga cagcaacatg ggaaacagaa ttgagagcat
     1561 cactcagcgc atggcaataa tagaaggaaa gaataagatg gctagcacct tggttgtggc
     1621 tgactctaga atttctggaa tctacatttg catagcttcc aataaagttg ggactgtggg
     1681 aagaaacata agcttttata tcacagatgt gccaaatggg tttcatgtta acttggaaaa
     1741 aatgccgacg gaaggagagg acctgaaact gtcttgcaca gttaacaagt tcttatacag
     1801 agacgttact tggattttac tgcggacagt taataacaga acaatgcact acagtattag
     1861 caagcaaaaa atggccatca ctaaggagca ctccatcact cttaatctta ccatcatgaa
     1921 tgtttccctg caagattcag gcacctatgc ctgcagagcc aggaatgtat acacagggga
     1981 agaaatcctc cagaagaaag aaattacaat cagagatcag gaagcaccat acctcctgcg
     2041 aaacctcagt gatcacacag tggccatcag cagttccacc actttagact gtcatgctaa
     2101 tggtgtcccc gagcctcaga tcacttggtt taaaaacaac cacaaaatac aacaagagcc
     2161 tggaattatt ttaggaccag gaagcagcac gctgtttatt gaaagagtca cagaagagga
     2221 tgaaggtgtc tatcactgca aagccaccaa ccagaagggc tctgtggaaa gttcagcata
     2281 cctcactgtt caaggaacct cggacaagtc taatctggag ctgatcactc taacatgcac
     2341 ctgtgtggct gcgactctct tctggctcct attaaccctc tttatccaaa aaatgaaaag
     2401 gtcttcttct gaaataaaga ctgactacct atcaattata atggacccag atgaagttcc
     2461 tttggatgag cagtgtgagc ggctccctta tgatgccagc aagtgggagt ttgcccggga
     2521 gagacttaaa ctgggcaaat cacttggaag aggggctttt ggaaaagtgg ttcaagcatc
     2581 agcatttggc attaagaaat cacctacgtg ccggactgtg gctgtgaaaa tgctgaaaga
     2641 gggggccacg gccagcgagt acaaagctct gatgactgag ctaaaaatct tgacccacat
     2701 tggccaccat ctgaacgtgg ttaacctgct gggagcctgc accaagcaag gagggcctct
     2761 gatggtgatt gttgaatact gcaaatatgg aaatctctcc aactacctca agagcaaacg
     2821 tgacttattt tttctcaaca aggatgcagc actacacatg gagcctaaga aagaaaaaat
     2881 ggagccaggc ctggaacaag gcaagaaacc aagactagat agcgtcacca gcagcgaaag
     2941 ctttgcgagc tccggctttc aggaagataa aagtctgagt gatgttgagg aagaggagga
     3001 ttctgacggt ttctacaagg agcccatcac tatggaagat ctgatttctt acagttttca
     3061 agtggccaga ggcatggagt tcctgtcttc cagaaagtgc attcatcggg acctggcagc
     3121 gagaaacatt cttttatctg agaacaacgt ggtgaagatt tgtgattttg gccttgcccg
     3181 ggatatttat aagaaccccg attatgtgag aaaaggagat actcgacttc ctctgaaatg
     3241 gatggctcct gaatctatct ttgacaaaat ctacagcacc aagagcgacg tgtggtctta
     3301 cggagtattg ctgtgggaaa tcttctcctt aggtgggtct ccatacccag gagtacaaat
     3361 ggatgaggac ttttgcagtc gcctgaggga aggcatgagg atgagagctc ctgagtactc
     3421 tactcctgaa atctatcaga tcatgctgga ctgctggcac agagacccaa aagaaaggcc
     3481 aagatttgca gaacttgtgg aaaaactagg tgatttgctt caagcaaatg tacaacagga
     3541 tggtaaagac tacatcccaa tcaatgccat actgacagga aatagtgggt ttacatactc
     3601 aactcctgcc ttctctgagg acttcttcaa ggaaagtatt tcagctccga agtttaattc
     3661 aggaagctct gatgatgtca gatacgtaaa tgctttcaag ttcatgagcc tggaaagaat
     3721 caaaaccttt gaagaacttt taccgaatgc cacctccatg tttgatgact accagggcga
     3781 cagcagcact ctgttggcct ctcccatgct gaagcgcttc acctggactg acagcaaacc
     3841 caaggcctcg ctcaagattg acttgagagt aaccagtaaa agtaaggagt cggggctgtc
     3901 tgatgtcagc aggcccagtt tctgccattc cagctgtggg cacgtcagcg aaggcaagcg
     3961 caggttcacc tacgaccacg ctgagctgga aaggaaaatc gcgtgctgct ccccgccccc
     4021 agactacaac tcggtggtcc tgtactccac cccacccatc tagaacccag ctttcttgta
     4081 caaagtggtg atatccaatt aaccctcact aaaggtagtt gaagttagtt taagtgttac
     4141 cctatagtga gtcgtattac cacccaactt ttctatacaa agtggttgat atccagcaca
     4201 gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc
     4261 tcctcggtct cgattctacg cgtaccggtt agtaatgaga tccctncccc cccccntaac
     4321 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     4381 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     4441 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     4501 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     4561 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     4621 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     4681 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     4741 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     4801 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     4861 cgatgataat atggccacac atatgaccga gtacaagccc acggtgcgcc tcgccacccg
     4921 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     4981 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     5041 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     5101 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     5161 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     5221 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     5281 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     5341 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     5401 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     5461 gacccgcaag cccggtgcct aaatcgatag atcctaatca acctctggat tacaaaattt
     5521 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     5581 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     5641 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     5701 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     5761 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     5821 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     5881 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     5941 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     6001 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     6061 tctccctttg ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt acaaggcagc
     6121 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     6181 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     6241 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6301 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6361 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     6421 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggcccgggt taattaagga
     6481 aagggctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     6541 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     6601 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6661 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     6721 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     6781 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     6841 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     6901 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     6961 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     7021 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     7081 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     7141 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     7201 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     7261 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     7321 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     7381 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     7441 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     7501 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7561 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     7621 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     7681 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7741 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7801 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7861 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7921 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7981 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     8041 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     8101 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     8161 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     8221 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     8281 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     8341 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     8401 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     8461 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     8521 cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct gactaatttt
     8581 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     8641 aggctttttt ggaggcctag gcttttgcaa aaagctcccc gtggcacgac aggtttcccg
     8701 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     8761 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     8821 aatttcacac aggaaacagc tatgacatga ttacgaattt cacaaataaa gcattttttt
     8881 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
     8941 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
     9001 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
     9061 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
     9121 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     9181 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     9241 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     9301 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     9361 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     9421 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     9481 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     9541 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     9601 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9661 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9721 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     9781 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     9841 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     9901 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     9961 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
    10021 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
    10081 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
    10141 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
    10201 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc
    10261 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
    10321 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
    10381 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
    10441 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
    10501 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
    10561 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
    10621 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
    10681 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
    10741 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
    10801 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
    10861 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
    10921 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
    10981 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
    11041 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
    11101 ttcgattagt gaacggatct cgacggtatc gccgaattca caaatggcag tattcatcca
    11161 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
    11221 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
    11281 tcgggtttat tacagggaca gcagagatcc agtttggact agtcgtgagg ctccggtgcc
    11341 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
    11401 aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
    11461 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
    11521 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc
    11581 cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg
    11641 ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg
    11701 ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
    11761 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
    11821 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
    11881 gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac
    11941 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
    12001 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
    12061 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
    12121 gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg
    12181 ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc
    12241 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc
    12301 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
    12361 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
    12421 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
    12481 ttttttcttc catttcaggt gtcgtga
//
