LOCUS       sequence-250731-        8955 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of CDKN2A A100S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8955)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 8955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-CDKN2A-A100S
FEATURES             Location/Qualifiers
     source          1..8955
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    199..316
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        364..1542
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          595..1533
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1490..1510
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1562..1586
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2056..2080)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2088..2106
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2131..2150)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2132..2150)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(2156..2176)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             2230..2271
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2292..2859
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2309..2861
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2453..2470)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2680..2699
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2873..3472
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2873..2892)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3369..3389
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3486..4074
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3539..3559)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3957..3968)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4149..4382
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4501..4519)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4587..4691
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4692..4760,4761..5552)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4910..4929)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5723..6311
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6212..6231
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6465..6482
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6722..6743
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6758..6763,6764..6781,6782..6788)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6796..6812
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6801..6823
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6820..6836
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6820..6836
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6886..6905
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7097..7730
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7777..7902
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8399..8632
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8817..8861
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
       61 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
      121 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccga attcacaaat
      181 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
      241 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      301 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggactagtcg
      361 tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
      421 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
      481 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
      541 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
      601 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
      661 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
      721 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
      781 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
      841 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
      901 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
      961 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
     1021 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
     1081 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
     1141 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
     1201 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
     1261 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
     1321 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1381 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1441 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1501 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaagcggccc tgcagatatc
     1561 aacaagtttg tacaaaaaag ttggcatgga gccggcggcg gggagcagca tggagccttc
     1621 ggctgactgg ctggccacgg ccgcggcccg gggtcgggta gaggaggtgc gggcgctgct
     1681 ggaggcgggg gcgctgccca acgcaccgaa tagttacggt cggaggccga tccaggtcat
     1741 gatgatgggc agcgcccgag tggcggagct gctgctgctc cacggcgcgg agcccaactg
     1801 cgccgacccc gccactctca cccgacccgt gcacgacgct gcccgggagg gcttcctgga
     1861 cacgctggtg gtgctgcacc ggtccggggc gcggctggac gtgcgcgatg cctggggccg
     1921 tctgcccgtg gacctggctg aggagctggg ccatcgcgat gtcgcacggt acctgcgcgc
     1981 ggctgcgggg ggcaccagag gcagtaacca tgcccgcata gatgccgcgg aaggtccctc
     2041 agatatcccc gattgaccca actttcttgt acaaagtggt gatatccaat taaccctcac
     2101 taaaggtgta tatgttaatg agatgttgta ccctatagtg agtcgtatta ccacccaact
     2161 tttctataca aagtggttga tatccagcac agtggcggcc gctcgagtct agagggcccg
     2221 cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt
     2281 tagtaatgag atccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     2341 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     2401 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     2461 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     2521 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     2581 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     2641 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     2701 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     2761 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     2821 cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacac atatgaccga
     2881 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     2941 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     3001 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     3061 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     3121 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     3181 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     3241 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     3301 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     3361 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     3421 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct aaatcgatag
     3481 atcctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     3541 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     3601 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     3661 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     3721 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     3781 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     3841 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     3901 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     3961 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     4021 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgaga
     4081 tcctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     4141 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     4201 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     4261 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     4321 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     4381 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     4441 agagagtgag aggcccgggt taattaagga aagggctaga tcattcttga agacgaaagg
     4501 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     4561 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4621 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4681 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4741 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4801 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4861 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4921 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     4981 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5041 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5101 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5161 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5221 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5281 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5341 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5401 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5461 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5521 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5581 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5641 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5701 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5761 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5821 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     5881 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5941 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6001 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6061 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6121 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6181 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6241 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6301 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6361 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     6421 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     6481 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     6541 aatgcagcaa gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6601 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     6661 aaagctcccc gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     6721 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     6781 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga
     6841 ttacgaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6901 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
     6961 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
     7021 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
     7081 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
     7141 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     7201 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     7261 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     7321 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     7381 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     7441 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     7501 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     7561 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7621 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7681 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     7741 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     7801 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     7861 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     7921 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     7981 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     8041 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     8101 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     8161 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     8221 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
     8281 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     8341 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     8401 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     8461 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     8521 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     8581 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     8641 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     8701 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     8761 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     8821 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     8881 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     8941 gaatagtttt tgctg
//
