LOCUS       sequence-250733-       12529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of FLT1 T777I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12529)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-FLT1-T777I
FEATURES             Location/Qualifiers
     source          1..12529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    20..44
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4065..4089)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4142..4183
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4204..4771
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4221..4773
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4365..4382)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4592..4611
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4785..4787,4788..4790,4791..5504)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4785..4787,4788..4790,4791..5501)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4830..4851)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5091..5110)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5438..5459
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5518..6106
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5571..5591)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5989..6000)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6181..6414
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6533..6551)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6619..6723
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6724..6792,6793..7584)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6942..6961)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7755..8343
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8244..8263
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8497..8514
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8754..8775
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8790..8795,8796..8813,8814..8820)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8828..8844
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8833..8855
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8852..8868
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8852..8868
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8918..8937
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9129..9762
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9809..9934
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10431..10664
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10849..10893
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11186..11303
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        11351..12529
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          11582..12520
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     12477..12497
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 agcggccctg cagatatcaa caagtttgta caaaaaagca ggcaccatgg tcagctactg
       61 ggacaccggg gtcctgctgt gcgcgctgct cagctgtctg cttctcacag gatctagttc
      121 aggttcaaaa ttaaaagatc ctgaactgag tttaaaaggc acccagcaca tcatgcaagc
      181 aggccagaca ctgcatctcc aatgcagggg ggaagcagcc cataaatggt ctttgcctga
      241 aatggtgagt aaggaaagcg aaaggctgag cataactaaa tctgcctgtg gaagaaatgg
      301 caaacaattc tgcagtactt taaccttgaa cacagctcaa gcaaaccaca ctggcttcta
      361 cagctgcaaa tatctagctg tacctacttc aaagaagaag gaaacagaat ctgcaatcta
      421 tatatttatt agtgatacag gtagaccttt cgtagagatg tacagtgaaa tccccgaaat
      481 tatacacatg actgaaggaa gggagctcgt cattccctgc cgggttacgt cacctaacat
      541 cactgttact ttaaaaaagt ttccacttga cactttgatc cctgatggaa aacgcataat
      601 ctgggacagt agaaagggct tcatcatatc aaatgcaacg tacaaagaaa tagggcttct
      661 gacctgtgaa gcaacagtca atgggcattt gtataagaca aactatctca cacatcgaca
      721 aaccaataca atcatagatg tccaaataag cacaccacgc ccagtcaaat tacttagagg
      781 ccatactctt gtcctcaatt gtactgctac cactcccttg aacacgagag ttcaaatgac
      841 ctggagttac cctgatgaaa aaaataagag agcttccgta aggcgacgaa ttgaccaaag
      901 caattcccat gccaacatat tctacagtgt tcttactatt gacaaaatgc agaacaaaga
      961 caaaggactt tatacttgtc gtgtaaggag tggaccatca ttcaaatctg ttaacacctc
     1021 agtgcatata tatgataaag cattcatcac tgtgaaacat cgaaaacagc aggtgcttga
     1081 aaccgtagct ggcaagcggt cttaccggct ctctatgaaa gtgaaggcat ttccctcgcc
     1141 ggaagttgta tggttaaaag atgggttacc tgcgactgag aaatctgctc gctatttgac
     1201 tcgtggctac tcgttaatta tcaaggacgt aactgaagag gatgcaggga attatacaat
     1261 cttgctgagc ataaaacagt caaatgtgtt taaaaacctc actgccactc taattgtcaa
     1321 tgtgaaaccc cagatttacg aaaaggccgt gtcatcgttt ccagacccgg ctctctaccc
     1381 actgggcagc agacaaatcc tgacttgtac cgcatatggt atccctcaac ctacaatcaa
     1441 gtggttctgg cacccctgta accataatca ttccgaagca aggtgtgact tttgttccaa
     1501 taatgaagag tcctttatcc tggatgctga cagcaacatg ggaaacagaa ttgagagcat
     1561 cactcagcgc atggcaataa tagaaggaaa gaataagatg gctagcacct tggttgtggc
     1621 tgactctaga atttctggaa tctacatttg catagcttcc aataaagttg ggactgtggg
     1681 aagaaacata agcttttata tcacagatgt gccaaatggg tttcatgtta acttggaaaa
     1741 aatgccgacg gaaggagagg acctgaaact gtcttgcaca gttaacaagt tcttatacag
     1801 agacgttact tggattttac tgcggacagt taataacaga acaatgcact acagtattag
     1861 caagcaaaaa atggccatca ctaaggagca ctccatcact cttaatctta ccatcatgaa
     1921 tgtttccctg caagattcag gcacctatgc ctgcagagcc aggaatgtat acacagggga
     1981 agaaatcctc cagaagaaag aaattacaat cagagatcag gaagcaccat acctcctgcg
     2041 aaacctcagt gatcacacag tggccatcag cagttccacc actttagact gtcatgctaa
     2101 tggtgtcccc gagcctcaga tcacttggtt taaaaacaac cacaaaatac aacaagagcc
     2161 tggaattatt ttaggaccag gaagcagcac gctgtttatt gaaagagtca cagaagagga
     2221 tgaaggtgtc tatcactgca aagccaccaa ccagaagggc tctgtggaaa gttcagcata
     2281 cctcactgtt caaggaacct cggacaagtc taatctggag ctgatcactc taacatgcac
     2341 ctgtgtggct gcgactctct tctggctcct attaatcctc tttatccgaa aaatgaaaag
     2401 gtcttcttct gaaataaaga ctgactacct atcaattata atggacccag atgaagttcc
     2461 tttggatgag cagtgtgagc ggctccctta tgatgccagc aagtgggagt ttgcccggga
     2521 gagacttaaa ctgggcaaat cacttggaag aggggctttt ggaaaagtgg ttcaagcatc
     2581 agcatttggc attaagaaat cacctacgtg ccggactgtg gctgtgaaaa tgctgaaaga
     2641 gggggccacg gccagcgagt acaaagctct gatgactgag ctaaaaatct tgacccacat
     2701 tggccaccat ctgaacgtgg ttaacctgct gggagcctgc accaagcaag gagggcctct
     2761 gatggtgatt gttgaatact gcaaatatgg aaatctctcc aactacctca agagcaaacg
     2821 tgacttattt tttctcaaca aggatgcagc actacacatg gagcctaaga aagaaaaaat
     2881 ggagccaggc ctggaacaag gcaagaaacc aagactagat agcgtcacca gcagcgaaag
     2941 ctttgcgagc tccggctttc aggaagataa aagtctgagt gatgttgagg aagaggagga
     3001 ttctgacggt ttctacaagg agcccatcac tatggaagat ctgatttctt acagttttca
     3061 agtggccaga ggcatggagt tcctgtcttc cagaaagtgc attcatcggg acctggcagc
     3121 gagaaacatt cttttatctg agaacaacgt ggtgaagatt tgtgattttg gccttgcccg
     3181 ggatatttat aagaaccccg attatgtgag aaaaggagat actcgacttc ctctgaaatg
     3241 gatggctcct gaatctatct ttgacaaaat ctacagcacc aagagcgacg tgtggtctta
     3301 cggagtattg ctgtgggaaa tcttctcctt aggtgggtct ccatacccag gagtacaaat
     3361 ggatgaggac ttttgcagtc gcctgaggga aggcatgagg atgagagctc ctgagtactc
     3421 tactcctgaa atctatcaga tcatgctgga ctgctggcac agagacccaa aagaaaggcc
     3481 aagatttgca gaacttgtgg aaaaactagg tgatttgctt caagcaaatg tacaacagga
     3541 tggtaaagac tacatcccaa tcaatgccat actgacagga aatagtgggt ttacatactc
     3601 aactcctgcc ttctctgagg acttcttcaa ggaaagtatt tcagctccga agtttaattc
     3661 aggaagctct gatgatgtca gatacgtaaa tgctttcaag ttcatgagcc tggaaagaat
     3721 caaaaccttt gaagaacttt taccgaatgc cacctccatg tttgatgact accagggcga
     3781 cagcagcact ctgttggcct ctcccatgct gaagcgcttc acctggactg acagcaaacc
     3841 caaggcctcg ctcaagattg acttgagagt aaccagtaaa agtaaggagt cggggctgtc
     3901 tgatgtcagc aggcccagtt tctgccattc cagctgtggg cacgtcagcg aaggcaagcg
     3961 caggttcacc tacgaccacg ctgagctgga aaggaaaatc gcgtgctgct ccccgccccc
     4021 agactacaac tcggtggtcc tgtactccac cccacccatc tagaacccag ctttcttgta
     4081 caaagtggtt gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga
     4141 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gttagtaatg
     4201 agatccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     4261 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     4321 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     4381 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     4441 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     4501 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     4561 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     4621 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     4681 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     4741 cgtggttttc ctttgaaaaa cacgatgata atatggccac acatatggtg agcaagggcg
     4801 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     4861 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     4921 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     4981 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     5041 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     5101 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     5161 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     5221 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     5281 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     5341 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     5401 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     5461 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaatcgat agatcctaat
     5521 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     5581 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     5641 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     5701 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     5761 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     5821 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     5881 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     5941 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     6001 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     6061 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctga gatcctttaa
     6121 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     6181 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     6241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     6421 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     6481 agaggcccgg gttaattaag gaaagggcta gatcattctt gaagacgaaa gggcctcgtg
     6541 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     6601 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     6661 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     6721 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     6781 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     6841 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     6901 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     6961 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     7021 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     7081 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7141 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     7201 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     7261 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     7321 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     7381 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     7441 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     7501 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     7561 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     7621 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     7681 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     7741 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     7801 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7861 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     7921 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7981 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     8041 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8101 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8161 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8221 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     8281 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     8341 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     8401 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     8461 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     8521 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     8581 aagctcatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     8641 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     8701 ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     8761 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     8821 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat
     8881 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8941 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
     9001 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt
     9061 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
     9121 ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat
     9181 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag
     9241 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga
     9301 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga
     9361 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt
     9421 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg
     9481 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc
     9541 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
     9601 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     9661 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     9721 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     9781 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     9841 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     9901 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     9961 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
    10021 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
    10081 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
    10141 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
    10201 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
    10261 cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat
    10321 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
    10381 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
    10441 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
    10501 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
    10561 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
    10621 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
    10681 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
    10741 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
    10801 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
    10861 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
    10921 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
    10981 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
    11041 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
    11101 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgccgaatt
    11161 cacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
    11221 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
    11281 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga
    11341 ctagtcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
    11401 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
    11461 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
    11521 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
    11581 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
    11641 gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
    11701 aagtgggtgg gggagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
    11761 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
    11821 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
    11881 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
    11941 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
    12001 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
    12061 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
    12121 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
    12181 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
    12241 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
    12301 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
    12361 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
    12421 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
    12481 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtga
//
