LOCUS       sequence-250735-        8955 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of CDKN2A H123Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8955)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 8955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-CDKN2A-H123Q
FEATURES             Location/Qualifiers
     source          1..8955
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    129..246
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        294..1472
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          525..1463
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1420..1440
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1492..1516
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1986..2010)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2018..2036
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2061..2080)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2062..2080)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(2086..2106)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             2160..2201
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2222..2789
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2239..2791
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2383..2400)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2610..2629
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2803..3402
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2803..2822)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3299..3319
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3416..4004
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3469..3489)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3887..3898)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4079..4312
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4431..4449)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4517..4621
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4622..4690,4691..5482)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4840..4859)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5653..6241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6142..6161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6395..6412
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6652..6673
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6688..6693,6694..6711,6712..6718)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6726..6742
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6731..6753
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6750..6766
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6750..6766
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6816..6835
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7027..7660
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7707..7832
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8329..8562
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8747..8791
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
       61 atccattcga ttagtgaacg gatctcgacg gtatcgccga attcacaaat ggcagtattc
      121 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      181 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      241 aattttcggg tttattacag ggacagcaga gatccagttt ggactagtcg tgaggctccg
      301 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
      361 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
      421 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
      481 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
      541 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
      601 cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt
      661 cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
      721 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
      781 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
      841 gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
      901 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
      961 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg
     1021 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
     1081 gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc
     1141 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag
     1201 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
     1261 cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     1321 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     1381 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc
     1441 aaagtttttt tcttccattt caggtgtcgt gaagcggccc tgcagatatc aacaagtttg
     1501 tacaaaaaag ttggcatgga gccggcggcg gggagcagca tggagccttc ggctgactgg
     1561 ctggccacgg ccgcggcccg gggtcgggta gaggaggtgc gggcgctgct ggaggcgggg
     1621 gcgctgccca acgcaccgaa tagttacggt cggaggccga tccaggtcat gatgatgggc
     1681 agcgcccgag tggcggagct gctgctgctc cacggcgcgg agcccaactg cgccgacccc
     1741 gccactctca cccgacccgt gcacgacgct gcccgggagg gcttcctgga cacgctggtg
     1801 gtgctgcacc gggccggggc gcggctggac gtgcgcgatg cctggggccg tctgcccgtg
     1861 gacctggctg aggagctggg ccaacgcgat gtcgcacggt acctgcgcgc ggctgcgggg
     1921 ggcaccagag gcagtaacca tgcccgcata gatgccgcgg aaggtccctc agatatcccc
     1981 gattgaccca actttcttgt acaaagtggt gatatccaat taaccctcac taaaggagta
     2041 agtgttagat agtattgaat ccctatagtg agtcgtatta ccacccaact tttctataca
     2101 aagtggttga tatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaag
     2161 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt tagtaatgag
     2221 atccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     2281 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     2341 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     2401 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     2461 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     2521 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     2581 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     2641 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     2701 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     2761 tggttttcct ttgaaaaaca cgatgataat atggccacac atatgaccga gtacaagccc
     2821 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     2881 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     2941 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     3001 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     3061 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     3121 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     3181 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     3241 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     3301 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     3361 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct aaatcgatag atcctaatca
     3421 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     3481 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     3541 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     3601 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     3661 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     3721 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     3781 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     3841 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     3901 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     3961 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga
     4021 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     4081 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     4141 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     4201 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     4261 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     4321 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     4381 aggcccgggt taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat
     4441 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     4501 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4561 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4621 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4681 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4741 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4801 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4861 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4921 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4981 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5041 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5101 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5161 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5221 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5281 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5341 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5401 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5461 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5521 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5581 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5641 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5701 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5761 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5821 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5881 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5941 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6001 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6061 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6121 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6181 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6241 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6301 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     6361 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     6421 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa
     6481 gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     6541 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc
     6601 gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     6661 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     6721 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt
     6781 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6841 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
     6901 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
     6961 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
     7021 agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct
     7081 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat
     7141 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag
     7201 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg
     7261 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg
     7321 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac
     7381 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc
     7441 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag
     7501 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7561 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7621 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     7681 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     7741 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     7801 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     7861 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     7921 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     7981 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     8041 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     8101 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     8161 cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     8221 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     8281 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     8341 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     8401 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     8461 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     8521 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     8581 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     8641 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     8701 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     8761 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     8821 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     8881 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     8941 ccacctccca acccc
//
