LOCUS       sequence-250736-       12506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of FLT1 V395I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12506)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-FLT1-V395I
FEATURES             Location/Qualifiers
     source          1..12506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    20..44
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4065..4089)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4097..4115
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4140..4159)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4141..4159)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(4165..4185)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             4239..4280
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4301..4868
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4318..4870
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4462..4479)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4689..4708
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4882..5481
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4882..4901)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5378..5398
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5495..6083
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5548..5568)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5966..5977)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6158..6391
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6510..6528)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6596..6700
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6701..6769,6770..7561)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6919..6938)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7732..8320
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8221..8240
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8474..8491
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8731..8752
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8767..8772,8773..8790,8791..8797)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8805..8821
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8810..8832
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8829..8845
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8829..8845
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8895..8914
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9106..9739
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9786..9911
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10408..10641
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10826..10870
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11163..11280
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        11328..12506
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          11559..12497
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     12454..12474
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 agcggccctg cagatatcaa caagtttgta caaaaaagca ggcaccatgg tcagctactg
       61 ggacaccggg gtcctgctgt gcgcgctgct cagctgtctg cttctcacag gatctagttc
      121 aggttcaaaa ttaaaagatc ctgaactgag tttaaaaggc acccagcaca tcatgcaagc
      181 aggccagaca ctgcatctcc aatgcagggg ggaagcagcc cataaatggt ctttgcctga
      241 aatggtgagt aaggaaagcg aaaggctgag cataactaaa tctgcctgtg gaagaaatgg
      301 caaacaattc tgcagtactt taaccttgaa cacagctcaa gcaaaccaca ctggcttcta
      361 cagctgcaaa tatctagctg tacctacttc aaagaagaag gaaacagaat ctgcaatcta
      421 tatatttatt agtgatacag gtagaccttt cgtagagatg tacagtgaaa tccccgaaat
      481 tatacacatg actgaaggaa gggagctcgt cattccctgc cgggttacgt cacctaacat
      541 cactgttact ttaaaaaagt ttccacttga cactttgatc cctgatggaa aacgcataat
      601 ctgggacagt agaaagggct tcatcatatc aaatgcaacg tacaaagaaa tagggcttct
      661 gacctgtgaa gcaacagtca atgggcattt gtataagaca aactatctca cacatcgaca
      721 aaccaataca atcatagatg tccaaataag cacaccacgc ccagtcaaat tacttagagg
      781 ccatactctt gtcctcaatt gtactgctac cactcccttg aacacgagag ttcaaatgac
      841 ctggagttac cctgatgaaa aaaataagag agcttccgta aggcgacgaa ttgaccaaag
      901 caattcccat gccaacatat tctacagtgt tcttactatt gacaaaatgc agaacaaaga
      961 caaaggactt tatacttgtc gtgtaaggag tggaccatca ttcaaatctg ttaacacctc
     1021 agtgcatata tatgataaag cattcatcac tgtgaaacat cgaaaacagc aggtgcttga
     1081 aaccgtagct ggcaagcggt cttaccggct ctctatgaaa gtgaaggcat ttccctcgcc
     1141 ggaagttgta tggttaaaag atgggttacc tgcgactgag aaatctgctc gctatttgac
     1201 tcgtggctac tcgttaatta tcaaggacat aactgaagag gatgcaggga attatacaat
     1261 cttgctgagc ataaaacagt caaatgtgtt taaaaacctc actgccactc taattgtcaa
     1321 tgtgaaaccc cagatttacg aaaaggccgt gtcatcgttt ccagacccgg ctctctaccc
     1381 actgggcagc agacaaatcc tgacttgtac cgcatatggt atccctcaac ctacaatcaa
     1441 gtggttctgg cacccctgta accataatca ttccgaagca aggtgtgact tttgttccaa
     1501 taatgaagag tcctttatcc tggatgctga cagcaacatg ggaaacagaa ttgagagcat
     1561 cactcagcgc atggcaataa tagaaggaaa gaataagatg gctagcacct tggttgtggc
     1621 tgactctaga atttctggaa tctacatttg catagcttcc aataaagttg ggactgtggg
     1681 aagaaacata agcttttata tcacagatgt gccaaatggg tttcatgtta acttggaaaa
     1741 aatgccgacg gaaggagagg acctgaaact gtcttgcaca gttaacaagt tcttatacag
     1801 agacgttact tggattttac tgcggacagt taataacaga acaatgcact acagtattag
     1861 caagcaaaaa atggccatca ctaaggagca ctccatcact cttaatctta ccatcatgaa
     1921 tgtttccctg caagattcag gcacctatgc ctgcagagcc aggaatgtat acacagggga
     1981 agaaatcctc cagaagaaag aaattacaat cagagatcag gaagcaccat acctcctgcg
     2041 aaacctcagt gatcacacag tggccatcag cagttccacc actttagact gtcatgctaa
     2101 tggtgtcccc gagcctcaga tcacttggtt taaaaacaac cacaaaatac aacaagagcc
     2161 tggaattatt ttaggaccag gaagcagcac gctgtttatt gaaagagtca cagaagagga
     2221 tgaaggtgtc tatcactgca aagccaccaa ccagaagggc tctgtggaaa gttcagcata
     2281 cctcactgtt caaggaacct cggacaagtc taatctggag ctgatcactc taacatgcac
     2341 ctgtgtggct gcgactctct tctggctcct attaaccctc tttatccgaa aaatgaaaag
     2401 gtcttcttct gaaataaaga ctgactacct atcaattata atggacccag atgaagttcc
     2461 tttggatgag cagtgtgagc ggctccctta tgatgccagc aagtgggagt ttgcccggga
     2521 gagacttaaa ctgggcaaat cacttggaag aggggctttt ggaaaagtgg ttcaagcatc
     2581 agcatttggc attaagaaat cacctacgtg ccggactgtg gctgtgaaaa tgctgaaaga
     2641 gggggccacg gccagcgagt acaaagctct gatgactgag ctaaaaatct tgacccacat
     2701 tggccaccat ctgaacgtgg ttaacctgct gggagcctgc accaagcaag gagggcctct
     2761 gatggtgatt gttgaatact gcaaatatgg aaatctctcc aactacctca agagcaaacg
     2821 tgacttattt tttctcaaca aggatgcagc actacacatg gagcctaaga aagaaaaaat
     2881 ggagccaggc ctggaacaag gcaagaaacc aagactagat agcgtcacca gcagcgaaag
     2941 ctttgcgagc tccggctttc aggaagataa aagtctgagt gatgttgagg aagaggagga
     3001 ttctgacggt ttctacaagg agcccatcac tatggaagat ctgatttctt acagttttca
     3061 agtggccaga ggcatggagt tcctgtcttc cagaaagtgc attcatcggg acctggcagc
     3121 gagaaacatt cttttatctg agaacaacgt ggtgaagatt tgtgattttg gccttgcccg
     3181 ggatatttat aagaaccccg attatgtgag aaaaggagat actcgacttc ctctgaaatg
     3241 gatggctcct gaatctatct ttgacaaaat ctacagcacc aagagcgacg tgtggtctta
     3301 cggagtattg ctgtgggaaa tcttctcctt aggtgggtct ccatacccag gagtacaaat
     3361 ggatgaggac ttttgcagtc gcctgaggga aggcatgagg atgagagctc ctgagtactc
     3421 tactcctgaa atctatcaga tcatgctgga ctgctggcac agagacccaa aagaaaggcc
     3481 aagatttgca gaacttgtgg aaaaactagg tgatttgctt caagcaaatg tacaacagga
     3541 tggtaaagac tacatcccaa tcaatgccat actgacagga aatagtgggt ttacatactc
     3601 aactcctgcc ttctctgagg acttcttcaa ggaaagtatt tcagctccga agtttaattc
     3661 aggaagctct gatgatgtca gatacgtaaa tgctttcaag ttcatgagcc tggaaagaat
     3721 caaaaccttt gaagaacttt taccgaatgc cacctccatg tttgatgact accagggcga
     3781 cagcagcact ctgttggcct ctcccatgct gaagcgcttc acctggactg acagcaaacc
     3841 caaggcctcg ctcaagattg acttgagagt aaccagtaaa agtaaggagt cggggctgtc
     3901 tgatgtcagc aggcccagtt tctgccattc cagctgtggg cacgtcagcg aaggcaagcg
     3961 caggttcacc tacgaccacg ctgagctgga aaggaaaatc gcgtgctgct ccccgccccc
     4021 agactacaac tcggtggtcc tgtactccac cccacccatc tagaacccag ctttcttgta
     4081 caaagtggtg atatccaatt aaccctcact aaagggagaa tgttgaataa gaagtaattc
     4141 cctatagtga gtcgtattac cacccaactt ttctatacaa agtggttgat atccagcaca
     4201 gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc
     4261 tcctcggtct cgattctacg cgtaccggtt agtaatgaga tccctccccc ccccctaacg
     4321 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     4381 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     4441 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     4501 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     4561 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     4621 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     4681 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     4741 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     4801 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     4861 gatgataata tggccacaca tatgaccgag tacaagccca cggtgcgcct cgccacccgc
     4921 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     4981 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     5041 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     5101 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     5161 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     5221 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     5281 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     5341 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     5401 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     5461 acccgcaagc ccggtgccta aatcgataga tcctaatcaa cctctggatt acaaaatttg
     5521 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     5581 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     5641 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     5701 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     5761 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     5821 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     5881 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     5941 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     6001 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     6061 ctccctttgg gccgcctccc cgcctgagat cctttaagac caatgactta caaggcagct
     6121 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     6181 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     6241 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     6301 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     6361 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     6421 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt aattaaggaa
     6481 agggctagat cattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     6541 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     6601 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     6661 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     6721 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     6781 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     6841 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     6901 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     6961 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     7021 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     7081 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     7141 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     7201 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     7261 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     7321 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     7381 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     7441 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     7501 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     7561 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     7621 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     7681 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     7741 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     7801 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     7861 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     7921 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     7981 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8041 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8101 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8161 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8221 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8281 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8341 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     8401 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     8461 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     8521 gcctctcccc gcgcgttggc cgattcatta atgcagcaag ctcatggctg actaattttt
     8581 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     8641 ggcttttttg gaggcctagg cttttgcaaa aagctccccg tggcacgaca ggtttcccga
     8701 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     8761 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     8821 atttcacaca ggaaacagct atgacatgat tacgaatttc acaaataaag catttttttc
     8881 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
     8941 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
     9001 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
     9061 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc
     9121 actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     9181 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
     9241 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
     9301 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
     9361 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
     9421 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
     9481 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
     9541 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     9601 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     9661 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     9721 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     9781 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     9841 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     9901 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     9961 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
    10021 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
    10081 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
    10141 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
    10201 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct
    10261 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
    10321 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
    10381 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
    10441 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
    10501 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
    10561 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
    10621 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
    10681 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
    10741 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
    10801 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
    10861 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
    10921 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
    10981 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
    11041 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
    11101 tcgattagtg aacggatctc gacggtatcg ccgaattcac aaatggcagt attcatccac
    11161 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
    11221 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
    11281 cgggtttatt acagggacag cagagatcca gtttggacta gtcgtgaggc tccggtgccc
    11341 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
    11401 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
    11461 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
    11521 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
    11581 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc
    11641 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
    11701 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
    11761 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
    11821 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
    11881 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
    11941 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
    12001 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
    12061 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
    12121 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
    12181 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
    12241 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
    12301 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
    12361 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
    12421 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
    12481 tttttcttcc atttcaggtg tcgtga
//
