LOCUS       sequence-250799-         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human oligoadenylate synthase 1 (OAS1) is used to label terminal 
            ADP-riobos.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Ando Y, Elkayam E, McPherson RL, Dasovich M, Cheng SJ, Voorneveld J,
            Filippov DV, Ong SE, Joshua-Tor L, Leung AKL
  TITLE     ELTA: Enzymatic Labeling of Terminal ADP-Ribose.
  JOURNAL   Mol Cell. 2019 Feb 21;73(4):845-856.e5. doi: 
            10.1016/j.molcel.2018.12.022. Epub 2019 Jan 31.
  PUBMED    30712989
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.12.022"; journalName: "Mol Cell"; date: 
            "2019-02-21- 21"; volume: "73"; issue: "4"; pages: "845-856.e5"
COMMENT     Sequence Label: pFl Strep-Sumo-TEV human OAS1
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(12..17,18..41,42..77,78..101)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             117..404
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated and modified)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="modified to prevent cleavage by eukaryotic SUMO 
                     proteases"
                     /translation="DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRR
                     LMEAFAKRQGKEMDSLTFLYDGIEIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             417..437
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gggcgcggat gtctgcttgg tcacaccctc aattcgagaa aggcggcggt agtggcggcg
       61 gatctggcgg tagtgcctgg agccatccgc agtttgaaaa gaccggttcc ctgcaggact
      121 cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct gagactcaca
      181 tcaatttaaa ggtgtccgat ggatcttcag agatcttctt caagatcaaa aagaccactc
      241 ctttaagaag gctgatggaa gcgttcgcta aaagacaggg taaggaaatg gactccttaa
      301 cattcttgta cgacggtatt gaaattcaag ctgatcagac ccctgaagat ttggacatgg
      361 aggataacga tattattgag gctcacagag aacagattgg tggaatccca acgaccgaaa
      421 acctgtattt tcagggcgcc atgatggacc tgcgtaacac cccggcgaag agcctggaca
      481 agtttattga ggattatctg ctgccggaca cctgctttcg tatgcaaatc aaccacgcga
      541 ttgacatcat ttgcggcttc ctgaaagagc gttgctttcg tggtagcagc tacccggtgt
      601 gcgttagcaa agtggttaag ggtggcagca gcggtaaagg taccaccctg cgtggccgta
      661 gcgatgcgga tctggtggtt ttcctgagcc cgctgaccac ctttcaggat caactgaacc
      721 gtcgtggtga gttcatccag gaaattcgtc gtcaactgga ggcgtgccag cgtgaacgtg
      781 cgttcagcgt gaaatttgaa gttcaagcgc cgcgttgggg taacccgcgt gcgctgagct
      841 ttgtgctgag cagcctgcag ctgggcgagg gtgtggagtt cgacgttctg ccggcgtttg
      901 atgcgctggg ccaactgacc ggtggctaca aaccgaaccc gcagatctat gttaagctga
      961 ttgaggaatg caccgacctg
//
