LOCUS       sequence-250815-        5794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encodes H. sapiens codon optimized Integrase 4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5794)
  AUTHORS   Gomide MS, Sales TT, Barros LRC, Limia CG, de Oliveira MA, 
            Florentino LH, Barros LMG, Robledo ML, Jose GPC, Almeida MSM, Lima 
            RN, Rehen SK, Lacorte C, Melo EO, Murad AM, Bonamino MH, Coelho CM, 
            Rech E
  TITLE     Genetic switches designed for eukaryotic cells and controlled by 
            serine integrases.
  JOURNAL   Commun Biol. 2020 May 22;3(1):255. doi: 10.1038/s42003-020-0971-8.
  PUBMED    32444777
REFERENCE   2  (bases 1 to 5794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-05-22"; volume: "3(1):255. doi"; pages: " 
            10.1038/s42003-020-0971-8"
COMMENT     Sequence Label: INCTbiosyn-pUB-HspINT4
FEATURES             Location/Qualifiers
     source          1..5794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        34..1245
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1082..1101
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     complement(2803..2822)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2868..2923
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2922..2941)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3284..3300)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3284..3300)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3297..3319)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3308..3324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3332..3338,3339..3356,3357..3362))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3377..3398
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3457..3652
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3503..3638
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3658..3792
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3695..3714)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3749..3768
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3860..3877)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4031..4619)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4111..4130)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4790..5581,5582..5650))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5413..5432
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5651..5755)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tattgactag taacccgtgt cggctccaga tctggcctcc gcgccgggtt ttggcgcctc
       61 ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc
      121 gtcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct
      181 tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac
      241 tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg
      301 cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg
      361 cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg
      421 atcgtcactt ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc
      481 gctcggtggg acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc
      541 aaggttgccc tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc
      601 ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg
      661 gtgggcggca agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg
      721 gtgagatggg ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga
      781 actcggtttg tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc
      841 cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat
      901 aatgcagggt ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg
      961 cagggttcgg gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg
     1021 gagggataag tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag
     1081 ctgaagctcc ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt
     1141 taggcacctt ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt
     1201 aaattgtccg ctaaattctg gccgtttttg gcttttttgt tagacgaagc ttgggctgca
     1261 ggtcgagctc aagcttcgaa ttcatgatca ccacacgcaa ggtggccata tacgtccgcg
     1321 tatctacgac taatcaagcc gaagagggat attctataca gggccaaatc gattccctta
     1381 tcaagtactg cgaggctatg ggttggatca tatatgagga atatactgac gcagggtttt
     1441 caggcggcaa aattgatcgg cccgccatga gtaagcttat tactgatgcc aaacacaaga
     1501 gattcgatac cattctggtg tacaaattgg atcggctgtc aagaagcgtg cgggatacac
     1561 tttacttggt taaggatgta tttaatcaaa acaacatcca cttcgtctcc cttcaggaga
     1621 atatcgacac atcttccgcg atgggaaatc tttttttgac cctcctttct gccatagcgg
     1681 agtttgagcg agagcagatc acggagcgca tgaccatggg gaaaataggt cgggccaaat
     1741 cagggaagac aatggcgtgg acctatacac cgttcggcta cgattataat aaggagaagg
     1801 gtgaacttat acttgatcct gccaaggcac ccatcgtgaa aatgatatac accgattact
     1861 tgaagggtat gtcaattcaa aagatagtgg ataaactcaa taaaatggat tacaacggca
     1921 aggattgcac atggtttccc cacggcgtga aacatctctt ggataatccg gtgtactacg
     1981 gtatgacaag atataacaat aagcttttcc ccggcaatca tcagccgata attacaaagg
     2041 agttgtttga taagacccag cgcgagagac aacggaggcg actcggcatc gaggaaaacc
     2101 attatacaat accgtttcag gcaaaatata tgctcagtaa gttcctcagg tgtcgccagt
     2161 gcggctcccg aatggggctt gaattgggtc gcccgcgcaa gaaggaaggc aagcgcagta
     2221 agaaatacta ctgcctcaat agccgaccga aacggacagc ttcatgtgac actcccctgt
     2281 atgacgccga aaccctggaa gattatgtgc tgcacgaaat agctaaaata cagaaagacc
     2341 cctcaatagc aagtagacag aagcatattg aagatcacga actcaaatat aagcgagaac
     2401 gcatagaggc aaatataaat aaaaccgtga atcaacttag caagctgaac aacctttacc
     2461 ttaacgacct cattactctt gaggacctca agacacagac taacacactc atcgcgaaaa
     2521 agcggctgtt ggagaatgaa cttgacaaga catgcgataa tgacgacgaa cttgacagac
     2581 aagagacaat agcggacttt ctggcattgc cagacgtttg gactatggac tatgaaggtc
     2641 aaaagtatgc cgtggagctt ctcgttcagc gcgtcaaagt tgacagagac aacatagaca
     2701 tacattggac gttttaagcg gccgcactcc tcaggtgcag gctgcctatc agaaggtggt
     2761 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa
     2821 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
     2881 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     2941 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
     3001 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca
     3061 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
     3121 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
     3181 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
     3241 atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg
     3301 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     3361 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     3421 ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat
     3481 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3541 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3601 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac
     3661 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3721 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3781 catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     3841 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     3901 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     3961 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     4021 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     4081 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     4141 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     4201 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     4261 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     4321 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     4381 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     4441 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     4501 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     4561 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     4621 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     4681 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     4741 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     4801 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     4861 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     4921 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     4981 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     5041 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     5101 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     5161 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     5221 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     5281 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     5341 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     5401 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     5461 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     5521 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     5581 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     5641 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     5701 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     5761 ttccccgaaa agtgccacct gggtcgacat tgat
//
