LOCUS       sequence-250816-        6019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encodes H. sapiens codon optimized Integrase 5..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Gomide MS, Sales TT, Barros LRC, Limia CG, de Oliveira MA, 
            Florentino LH, Barros LMG, Robledo ML, Jose GPC, Almeida MSM, Lima 
            RN, Rehen SK, Lacorte C, Melo EO, Murad AM, Bonamino MH, Coelho CM, 
            Rech E
  TITLE     Genetic switches designed for eukaryotic cells and controlled by 
            serine integrases.
  JOURNAL   Commun Biol. 2020 May 22;3(1):255. doi: 10.1038/s42003-020-0971-8.
  PUBMED    32444777
REFERENCE   2  (bases 1 to 6019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-05-22"; volume: "3(1):255. doi"; pages: " 
            10.1038/s42003-020-0971-8"
COMMENT     Sequence Label: INCTbiosyn-pUB-HspINT5
FEATURES             Location/Qualifiers
     source          1..6019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        47..1258
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1095..1114
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     complement(3041..3060)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3106..3161
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3160..3179)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3522..3538)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3522..3538)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3535..3557)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3546..3562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3570..3576,3577..3594,3595..3600))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3615..3636
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3695..3890
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3741..3876
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3896..4030
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3933..3952)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3987..4006
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4098..4115)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4269..4857)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4349..4368)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5028..5819,5820..5888))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5651..5670
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5889..5993)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagtaacccg tgtcggctcc agatctggcc tccgcgccgg
       61 gttttggcgc ctcccgcggg cgcccccctc ctcacggcga gcgctgccac gtcagacgaa
      121 gggcgcagcg agcgtcctga tccttccgcc cggacgctca ggacagcggc ccgctgctca
      181 taagactcgg ccttagaacc ccagtatcag cagaaggaca ttttaggacg ggacttgggt
      241 gactctaggg cactggtttt ctttccagag agcggaacag gcgaggaaaa gtagtccctt
      301 ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt atataaggac
      361 gcgccgggtg tggcacagct agttccgtcg cagccgggat ttgggtcgcg gttcttgttt
      421 gtggatcgct gtgatcgtca cttggtgagt agcgggctgc tgggctggcc ggggctttcg
      481 tggccgccgg gccgctcggt gggacggaag cgtgtggaga gaccgccaag ggctgtagtc
      541 tgggtccgcg agcaaggttg ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg
      601 ctgttcccga gtcttgaatg gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa
      661 ggtggggggc atggtgggcg gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa
      721 agctcttatt cgggtgagat gggctggggc accatctggg gaccctgacg tgaagtttgt
      781 cactgactgg agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt
      841 tgggcagtgc acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt
      901 tctgttggct tataatgcag ggtggggcca cctgccggta ggtgtgcggt aggcttttct
      961 ccgtcgcagg acgcagggtt cgggcctagg gtaggctctc ctgaatcgac aggcgccgga
     1021 cctctggtga ggggagggat aagtgaggcg tcagtttctt tggtcggttt tatgtaccta
     1081 tcttcttaag tagctgaagc tccggttttg aactatgcgc tcggggttgg cgagtgtgtt
     1141 ttgtgaagtt ttttaggcac cttttgaaat gtaatcattt gggtcaatat gtaattttca
     1201 gtgttagact agtaaattgt ccgctaaatt ctggccgttt ttggcttttt tgttagacga
     1261 agcttgggct gcaggtcgag ctcaagcttc gaattcatgc cgggaatgac cacggaaacc
     1321 ggaccagatc cggcaggttt gatagacctg ttttgccgca aatctaaggc agtgaaaagc
     1381 cgggctaatg gagctgggca acggagaaaa caggaaatct ctattgctgc tcaggagacg
     1441 ctggggcgca aggtcgctgc cctgctcggc atgcaagtcc gccatgtgtg gaaggaagta
     1501 ggtagcgcga gtcgattcag aaagggaaag gcaagggatg atcagagtaa agcccttaaa
     1561 gcacttgaga gtggggaggt cggggcgctc tggtgctatc ggcttgaccg atgggatagg
     1621 ggaggcgcgg gagcaattct taaaattata gagcccgagg atggcatgcc gcgaagattg
     1681 cttttcggct gggatgagga tacaggtcga cccgtattgg attctacaaa taaaagggac
     1741 cggggtgagc tgatcagacg agcggaggaa gcccgagaag aagcagaaaa actgtcagag
     1801 cgcgtccgcg atactaaggc acatcagcgc gagaacgggg aatgggtaaa cgcgcgcgca
     1861 ccatatggcc tccgagtggt cttggtgact gttagtgatg aggagggcga cgagtatgat
     1921 gaaaggaaat tggctgcaga cgatgaagat gctggtggac ccgatggtct cacaaaagct
     1981 gaagcggcac ggctggtctt cactctcccg gttaccgacc gactttccta cgcaggtacc
     2041 gcccatgcaa tgaacactcg ggaaattcca tcaccgacag gcggtccatg gatagctgtg
     2101 acagttaggg acatgatcca gaaccccgct tatgcggggt ggcagaccac aggcaggcag
     2161 gatggaaaac aaaggcgctt gacattctac aacggagagg ggaaaagggt ctctgtcatg
     2221 cacggcccac ccctggtaac agatgaagaa caggaagcgg ccaaggcggc agtcaaaggc
     2281 gaagacggag taggtgtgcc gcttgacggc tcagatcatg acacacgccg caaacatctc
     2341 ctcagtggta ggatgcgatg tccgggatgt ggtggctcat gctcctactc aggtaacggt
     2401 taccggtgtt ggagatctag tgttaaaggg ggctgtccag caccgacgta cgtggccaga
     2461 aagtcagtcg aggagtatgt ggcttttagg tgggccgcta aactggcggc ctccgagcct
     2521 gatgacccct ttgtaatagc tgtggcagat cgctgggcag ctttgacaca cccacaggct
     2581 tccgaagatg aaaaatacgc taaagctgcg gttcgagaag cagagaagaa tcttggccgg
     2641 ctgctgcgag accggcagaa cggcgtctat gatgggcccg cggagcagtt ctttgcgccg
     2701 gcttatcaag aagccctttc aacgctgcag gcggctaagg atgctgtctc agaaagctcc
     2761 gcatccgcag cagtggatgt aagttggata gtggatagtt ccgattacga ggagctgtgg
     2821 cttcgggcga ccccgacaat gaggaatgcg attattgata cttgcatcga cgagatttgg
     2881 gtggcaaagg gacaacgagg gagaccgttt gatggagatg aacgggtcaa gattaaatgg
     2941 gcagctcgga cgtaagcggc cgcactcctc aggtgcaggc tgcctatcag aaggtggtgg
     3001 ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa
     3061 attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta
     3121 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     3181 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc
     3241 atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc
     3301 cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta
     3361 tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac
     3421 tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat
     3481 ggagatccct cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     3541 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     3601 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     3661 ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag
     3721 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     3781 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     3841 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt
     3901 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     3961 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     4021 tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     4081 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     4141 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     4201 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     4261 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     4321 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     4381 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     4441 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     4501 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     4561 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     4621 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     4681 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     4741 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     4801 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     4861 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     4921 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     4981 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     5041 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     5101 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     5161 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     5221 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     5281 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     5341 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     5401 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     5461 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     5521 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     5581 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     5641 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     5701 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     5761 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     5821 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     5881 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     5941 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     6001 ccccgaaaag tgccacctg
//
