LOCUS       sequence-250817-        5718 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encodes H. sapiens codon optimized Integrase 7..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Gomide MS, Sales TT, Barros LRC, Limia CG, de Oliveira MA, 
            Florentino LH, Barros LMG, Robledo ML, Jose GPC, Almeida MSM, Lima 
            RN, Rehen SK, Lacorte C, Melo EO, Murad AM, Bonamino MH, Coelho CM, 
            Rech E
  TITLE     Genetic switches designed for eukaryotic cells and controlled by 
            serine integrases.
  JOURNAL   Commun Biol. 2020 May 22;3(1):255. doi: 10.1038/s42003-020-0971-8.
  PUBMED    32444777
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-05-22"; volume: "3(1):255. doi"; pages: " 
            10.1038/s42003-020-0971-8"
COMMENT     Sequence Label: INCTbiosyn-pUB-HspINT7
FEATURES             Location/Qualifiers
     source          1..5718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        59..1270
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1107..1126
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     polyA_signal    2817..2872
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2871..2890)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3233..3249)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3233..3249)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3246..3268)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3257..3273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3281..3287,3288..3305,3306..3311))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3326..3347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3406..3601
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3452..3587
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3607..3741
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3644..3663)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3698..3717
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3809..3826)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3980..4568)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4060..4079)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4739..5530,5531..5599))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5362..5381
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5600..5704)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aagtgccacc tgggtcgaca ttgattattg actagtaacc cgtgtcggct ccagatctgg
       61 cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc
      121 acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct caggacagcg
      181 gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga cattttagga
      241 cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac aggcgaggaa
      301 aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga acgccgatga
      361 ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg atttgggtcg
      421 cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct gctgggctgg
      481 ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga gagaccgcca
      541 agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg ggggagcgca
      601 gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg
      661 tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt gaggccttcg
      721 ctaatgcggg aaagctctta ttcgggtgag atgggctggg gcaccatctg gggaccctga
      781 cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta
      841 tggcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt
      901 gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg taggtgtgcg
      961 gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg
     1021 acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc tttggtcggt
     1081 tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc gctcggggtt
     1141 ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat ttgggtcaat
     1201 atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt
     1261 tttgttagac gaagcttggg ctgcaggtcg agctcaagct tcgaattcat gaaggtagct
     1321 atatacgtcc gcgtctcaac agatgaacaa gctaaggagg gattctccat tccggcgcag
     1381 agagagaggc tcagggcttt ttgtgccagt caagggtggg agatcgtgca agaatatatc
     1441 gaagagggct ggagcgcaaa ggaccttgat cgcccacaga tgcagcgatt gctgaaggac
     1501 ataaaaaaag ggaacataga catagtgctg gtctataggc ttgaccgact taccaggtca
     1561 gtcctcgatc tctacctgtt gctccaaaca ttcgaaaagt ataacgtagc cttccgatcc
     1621 gctaccgagg tttacgacac aagcacagct atggggcgac tttttattac cttggttgcc
     1681 gctttggcgc aatgggaacg cgaaaatctc gcagagagag taaagttcgg catagaacag
     1741 atgatcgatg aaggcaaaaa gcctggtggg cattcaccat atggctataa gttcgataaa
     1801 gacttcaact gtacgattat tgaagaggag gcagacgttg ttaggatgat ataccgcatg
     1861 tactgcgatg gatatggcta taggagtatt gccgatcgac tgaatgagct tatggtaaaa
     1921 ccaagaatag ctaaagagtg gaatcataac agtgttcgcg acatccttac taacgacata
     1981 tatatcggca cgtaccgatg gggagacaag gtggttccaa acaaccaccc acctatcatt
     2041 agtgaaactt tgtttaagaa agcgcaaaaa gaaaaagaaa agcgaggagt ggatcgaaag
     2101 agagttggta agttcctgtt tactggtctt ctccagtgtt gtaattgtgg aggacacaag
     2161 atgcaggggc acttcgacaa acgcgagcaa aagacttact acagatgcac gaagtgtcac
     2221 aggattacca atgagaaaaa tatcctggaa cccttgttgg atgagataca gttgctgata
     2281 acatccaagg aatattttat gtctaaattt agtgaccgat atgatcaaca ggaagtagta
     2341 gacgtttctg ctcttacgaa agaactggag aagatcaagc gccaaaagga gaagtggtat
     2401 gatctctata tggacgatcg caatcccatt cctaaggaag agttgtttgc gaaaatcaat
     2461 gaactgaaca agaaagaaga agagatatat tccaagttga gcgaggtaga ggaggacaaa
     2521 gagcccgtcg aagagaaatg caatagactg tccaagatga ttgattttaa gcagcaattc
     2581 gaacaggcga acgacttcac gaaaaaggaa ttgttgttta gcatatttga aaaaatcgta
     2641 atttataggg aaaaaggtaa gctgaagaag ataactctgg actacactct caagtaagcg
     2701 gccgcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
     2761 tggctcacaa ataccactga gatctttttc cctctgccta tcagaaggtg gtggctaata
     2821 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     2881 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
     2941 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta
     3001 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
     3061 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
     3121 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
     3181 tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc
     3241 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     3301 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     3361 cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc
     3421 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3481 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3541 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     3601 aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3661 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3721 gtatcttatc atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     3781 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     3841 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3901 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3961 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4021 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4081 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4141 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4201 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4261 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4321 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4381 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     4441 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4501 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     4561 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     4621 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     4681 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     4741 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     4801 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     4861 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     4921 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     4981 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     5041 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     5101 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     5161 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     5221 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     5281 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     5341 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     5401 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     5461 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     5521 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     5581 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     5641 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     5701 cgcgcacatt tccccgaa
//
