LOCUS       sequence-250818-        5746 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encodes H. sapiens codon optimized Integrase 9..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Gomide MS, Sales TT, Barros LRC, Limia CG, de Oliveira MA, 
            Florentino LH, Barros LMG, Robledo ML, Jose GPC, Almeida MSM, Lima 
            RN, Rehen SK, Lacorte C, Melo EO, Murad AM, Bonamino MH, Coelho CM, 
            Rech E
  TITLE     Genetic switches designed for eukaryotic cells and controlled by 
            serine integrases.
  JOURNAL   Commun Biol. 2020 May 22;3(1):255. doi: 10.1038/s42003-020-0971-8.
  PUBMED    32444777
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-05-22"; volume: "3(1):255. doi"; pages: " 
            10.1038/s42003-020-0971-8"
COMMENT     Sequence Label: INCTbiosyn-pUB-HspINT9
FEATURES             Location/Qualifiers
     source          1..5746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        92..1303
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1140..1159
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     complement(2813..2832)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2878..2933
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2932..2951)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3294..3310)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3294..3310)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3307..3329)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3318..3334
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3342..3348,3349..3366,3367..3372))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3387..3408
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3467..3662
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3513..3648
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3668..3802
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3705..3724)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3759..3778
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3870..3887)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4041..4629)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4121..4140)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4800..5591,5592..5660))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5423..5442
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(5661..5746,1..19))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg acattgatta
       61 ttgactagta acccgtgtcg gctccagatc tggcctccgc gccgggtttt ggcgcctccc
      121 gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt
      181 cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta
      241 gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg
      301 gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg
      361 gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca
      421 cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat
      481 cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc
      541 tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa
      601 ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt cccgagtctt
      661 gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt
      721 gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc ttattcgggt
      781 gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac
      841 tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc agtgcacccg
      901 tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa
      961 tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc gcaggacgca
     1021 gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga
     1081 gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct
     1141 gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta
     1201 ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa
     1261 attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt gggctgcagg
     1321 tcgagctcaa gcttcgaatt catgaaggtg gccatttaca cgcgagtgag tactttggaa
     1381 cagaaggaga aggggcatag tattgaggaa caggaacgca agctgcgggc ctacagtgac
     1441 atcaacgatt ggaaaatcca taaagtatac actgacgcag gatacagcgg ggcgaaaaag
     1501 gatcgcccag cacttcagga aatgttgaat gagattgata attttgacct cgtcctcgtc
     1561 tacaagttgg ataggcttac aaggagtgtg aaagacctcc ttgagattct cgagctgttt
     1621 gaaaacaaga acgtcctgtt tagatcagcc actgaggtct acgatactac gtcagctatg
     1681 ggtcggctct ttgttactct ggttggcgct atggctgagt gggaacgcac gacaattcag
     1741 gagagaactg cgatggggcg acgagcgagt gctcggaaag gacttgccaa gactgttcca
     1801 ccgttttact acgacagggt gaatgataag tttgttccca acgaatataa aaaggtcctg
     1861 cgcttcgcgg tcgaagaggc aaaaaaaggg acaagcttgc gagagatcac cattaaactg
     1921 aataactcca agtataaagc tccattgggc aagaattggc atcgaagtgt gataggaaat
     1981 gcactgacga gcccggttgc cagggggcac ttggtctttg gcgacatttt tgtcgagaac
     2041 acccatgagg cgattataag tgaagaagag tacgaggaga taaaactcag gatctctgag
     2101 aaaacgaata gcacaatagt taagcacaac gccatattca gatcaaaact gctgtgtccc
     2161 aattgcaacc agaagctgac actgaatacc gtgaaacata ccccaaagaa caaggaggtt
     2221 tggtattcta aactgtactt ctgcagtaat tgcaagaaca caaagaacaa aaatgcatgc
     2281 aacattgacg agggagaggt gctgaagcaa ttttataatt acctgaagca atttgacctg
     2341 acgtcctaca aaatagaaaa ccagccgaaa gaaattgagg atgtggggat cgacatcgaa
     2401 aagcttcgca aggagcgggc acgatgccag acacttttca ttgagggcat gatggacaaa
     2461 gatgaagctt ttcccatcat ttcaagaata gataaggaga tccatgagta tgagaagcgg
     2521 aaggataacg acaaggggaa gacgttcaac tacgagaaga taaagaattt caagtactcc
     2581 cttcttaatg ggtgggaact catggaagac gagctgaaaa cggaattcat caaaatggct
     2641 atcaaaaaca tccacttcga gtatgtgaag gggattaaag gtaaaagaca aaatagtctc
     2701 aagataaccg gtatagagtt ttactaagcg gccgcactcc tcaggtgcag gctgcctatc
     2761 agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc
     2821 cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact tctggctaat
     2881 aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa
     2941 ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg
     3001 caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt
     3061 atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt
     3121 agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct
     3181 tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc
     3241 ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag
     3301 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     3361 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     3421 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt
     3481 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     3541 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     3601 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     3661 aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3721 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3781 tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc
     3841 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     3901 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     3961 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     4021 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     4081 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     4141 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     4201 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     4261 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     4321 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     4381 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     4441 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     4501 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     4561 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     4621 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     4681 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     4741 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     4801 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     4861 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     4921 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     4981 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     5041 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     5101 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     5161 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     5221 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     5281 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     5341 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     5401 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     5461 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     5521 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     5581 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     5641 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     5701 tattgtctca tgagcggata catatttgaa tgtatttaga aaaata
//
