LOCUS       sequence-250825-        6148 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encodes H. sapiens codon optimized  Integrase Bxb1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Gomide MS, Sales TT, Barros LRC, Limia CG, de Oliveira MA, 
            Florentino LH, Barros LMG, Robledo ML, Jose GPC, Almeida MSM, Lima 
            RN, Rehen SK, Lacorte C, Melo EO, Murad AM, Bonamino MH, Coelho CM, 
            Rech E
  TITLE     Genetic switches designed for eukaryotic cells and controlled by 
            serine integrases.
  JOURNAL   Commun Biol. 2020 May 22;3(1):255. doi: 10.1038/s42003-020-0971-8.
  PUBMED    32444777
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-05-22"; volume: "3(1):255. doi"; pages: " 
            10.1038/s42003-020-0971-8"
COMMENT     Sequence Label: INCTbiosyn-pUB-HspINTBxb1
FEATURES             Location/Qualifiers
     source          1..6148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     56..73
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    190..211
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(226..231,232..249,250..256)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    264..280
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     269..291
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     288..304
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     288..304
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        325..343
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        362..1573
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1410..1429
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     complement(3168..3187)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3233..3288
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3287..3306)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3660..3679)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3661..3679)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3686..3703)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3686..3702)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3695..3717)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(3844..4299)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3981..4002)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4193..4212
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4326..4430
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4431..4499,4500..5291)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4649..4668)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5462..6050
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5951..5970
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
       61 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
      121 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
      181 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
      241 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
      301 tgaccatgat tacgccaagc tcgaaattaa ccctcactaa agggaacaaa agctggagct
      361 cggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct
      421 gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac gctcaggaca
      481 gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa ggacatttta
      541 ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg aacaggcgag
      601 gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg tgaacgccga
      661 tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc gggatttggg
      721 tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc
      781 tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg
      841 ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt tggggggagc
      901 gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg
      961 aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt cttgaggcct
     1021 tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat ctggggaccc
     1081 tgacgtgaag tttgtcactg actggagaac tcgggtttgt cgtctggttg cgggggcggc
     1141 agttatgcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc ctcgtcgtgt
     1201 cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc cggtaggtgt
     1261 gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg ctctactgaa
     1321 tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt ttctttggtc
     1381 ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta tgcgctcggg
     1441 gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat catttgggtc
     1501 aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc
     1561 ttttttgtta gacgaattca tgcgggcact ggtggtaatt aggctctcca gagttactga
     1621 cgctacgacc agccctgaga ggcaacttga aagctgccag cagctctgtg cgcaacgggg
     1681 ctgggacgtc gttggagtgg cggaagacct cgatgtttcc ggtgcagtag atcccttcga
     1741 taggaagcgg cgcccgaatt tggctcgatg gctcgctttc gaagagcaac cattcgatgt
     1801 gatcgttgcc tatcgagtcg acaggttgac aagaagtatt cgccatcttc aacaattggt
     1861 tcattgggca gaagatcaca aaaaattggt tgtctccgct acggaagcac actttgatac
     1921 aactacaccg tttgcagcag ttgtcatagc tctgatgggt accgttgcac aaatggaatt
     1981 ggaagctatt aaagagcgaa atcggagcgc cgcgcacttt aacatcaggg caggcaagta
     2041 ccgcggcagt ctcccaccct ggggctacct gccgactaga gtggatggcg aatggcggtt
     2101 ggtgcccgac ccggtccaac gggaaagaat ccttgaagtg taccatcgag ttgtggacaa
     2161 tcacgagccc cttcacctgg tagctcatga tttgaatcgc cggggagttt tgagcccgaa
     2221 agattatttc gcccagctgc aagggaggga accacagggt cgggagtgga gtgctactgc
     2281 tctgaaaaga tccatgatta gcgaagccat gctgggatac gcaaccctta acggcaaaac
     2341 cgttagggac gatgacggag cgcccctggt tcgggccgaa ccaattctta ctcgcgagca
     2401 actggaagca ctgcgggctg agctggtcaa aacatcacgg gctaaaccgg cggtgagcac
     2461 accaagtttg ttgcttcgag tattgttttg cgccgtttgt ggagagccag catacaagtt
     2521 tgccgggggt gggagaaaac atccccgcta tagatgccgc agcatgggct tcccgaagca
     2581 ttgtggaaac ggtacagtag cgatggctga gtgggatgca ttctgcgaag aacaagtcct
     2641 tgacttgctt ggcgacgcag aacgcctgga aaaggtatgg gtagctgggt ccgactccgc
     2701 tgtagaactt gctgaagtca atgcagaact ggtcgatctc acatccctta ttggctctcc
     2761 agcgtaccga gctggctccc cgcaacgcga ggcacttgat gccagaatag cggcactcgc
     2821 cgcgagacaa gaggaattgg aggggttgga agctagacct tctggatggg aatggaggga
     2881 aacaggccag cggttcgggg attggtggcg cgagcaagat actgcggcga agaatacgtg
     2941 gcttaggagc atgaacgtcc ggctgacgtt cgacgttaga ggggggctta ccaggactat
     3001 cgatttcgga gatttgcagg agtatgaaca acacttgagg cttggtagcg tagtggagag
     3061 actgcataca gggatgtctt aggcggccgc actcctcagg tgcaggctgc ctatcagaag
     3121 gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct
     3181 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
     3241 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
     3301 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
     3361 tatgccatat gctggctgcc atgaacaaag gtggctataa agaggtcatc agtatatgaa
     3421 acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt
     3481 ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt
     3541 tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct
     3601 cttatgaaga tccctcgacc tgcagcccaa gcttggcgta atcatgggta cccaattcgc
     3661 cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     3721 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     3781 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     3841 ggcaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     3901 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     3961 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     4021 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     4081 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     4141 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     4201 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     4261 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
     4321 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4381 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4441 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4501 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4561 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     4621 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     4681 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4741 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4801 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4861 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4921 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4981 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5041 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5101 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     5161 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5221 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     5281 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5341 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5401 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5461 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5521 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5581 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     5641 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5701 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5761 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5821 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5881 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5941 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6001 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6061 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     6121 cgttatcccc tgattctgtg gataaccg
//
