LOCUS       sequence-250860-        1185 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human cGAS-Delta-N (aa160-522)-HA with an N terminal 
            fusion of the Fyn dual acylation motif (aa1-16); Puromycin selection
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Barnett KC, Coronas-Serna JM, Zhou W, Ernandes MJ, Cao A, Kranzusch 
            PJ, Kagan JC
  TITLE     Phosphoinositide Interactions Position cGAS at the Plasma Membrane 
            to Ensure Efficient Distinction between Self- and Viral DNA.
  JOURNAL   Cell. 2019 Mar 7;176(6):1432-1446.e11. doi: 
            10.1016/j.cell.2019.01.049. Epub 2019 Feb 28.
  PUBMED    30827685
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.01.049"; journalName: "Cell"; date: "2019-03-7-
            7"; volume: "176"; issue: "6"; pages: "1432-1446.e11"
COMMENT     Sequence Label: pLenti-CMV-Fyn-cGAS-Delta-N-HA
FEATURES             Location/Qualifiers
     source          1..1185
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1150..1176
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tctagagcca ccatgggctg tgtgcaatgt aaggataaag aagcaacaaa actgacggag
       61 cctggggcct cgaagctccg ggcggttttg gagaagttga agctcagccg cgatgatatc
      121 tccacggcgg cggggatggt gaaaggggtt gtggaccacc tgctgctcag actgaagtgc
      181 gactccgcgt tcagaggcgt cgggctgctg aacaccggga gctactatga gcacgtgaag
      241 atttctgcac ctaatgaatt tgatgtcatg tttaaactgg aagtccccag aattcaacta
      301 gaagaatatt ccaacactcg tgcatattac tttgtgaaat ttaaaagaaa tccgaaagaa
      361 aatcctctga gtcagttttt agaaggtgaa atattatcag cttctaagat gctgtcaaag
      421 tttaggaaaa tcattaagga agaaattaac gacattaaag atacagatgt catcatgaag
      481 aggaaaagag gagggagccc tgctgtaaca cttcttatta gtgaaaaaat atctgtggat
      541 ataaccctgg ctttggaatc aaaaagtagc tggcctgcta gcacccaaga aggcctgcgc
      601 attcaaaact ggctttcagc aaaagttagg aagcaactac gactaaagcc attttacctt
      661 gtacccaagc atgcaaagga aggaaatggt ttccaagaag aaacatggcg gctatccttc
      721 tctcacatcg aaaaggaaat tttgaacaat catggaaaat ctaaaacgtg ctgtgaaaac
      781 aaagaagaga aatgttgcag gaaagattgt ttaaaactaa tgaaatacct tttagaacag
      841 ctgaaagaaa ggtttaaaga caaaaaacat ctggataaat tctcttctta tcatgtgaaa
      901 actgccttct ttcacgtatg tacccagaac cctcaagaca gtcagtggga ccgcaaagac
      961 ctgggcctct gctttgataa ctgcgtgaca tactttcttc agtgcctcag gacagaaaaa
     1021 cttgagaatt attttattcc tgaattcaat ctattctcta gcaacttaat tgacaaaaga
     1081 agtaaggaat ttctgacaaa gcaaattgaa tatgaaagaa acaatgagtt tccagttttt
     1141 gatgaatttt acccatacga tgttccagat tacgcttgag tcgac
//
