LOCUS       sequence-250862-        1392 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human cGAS-Delta-N (aa160-522)-HA with an N terminal 
            fusion of the TIRAP N terminus (aa1-85); Puromycin selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Barnett KC, Coronas-Serna JM, Zhou W, Ernandes MJ, Cao A, Kranzusch 
            PJ, Kagan JC
  TITLE     Phosphoinositide Interactions Position cGAS at the Plasma Membrane 
            to Ensure Efficient Distinction between Self- and Viral DNA.
  JOURNAL   Cell. 2019 Mar 7;176(6):1432-1446.e11. doi: 
            10.1016/j.cell.2019.01.049. Epub 2019 Feb 28.
  PUBMED    30827685
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.01.049"; journalName: "Cell"; date: "2019-03-7-
            7"; volume: "176"; issue: "6"; pages: "1432-1446.e11"
COMMENT     Sequence Label: pLenti-CMV-TIRAP-cGAS-Delta-N-HA
FEATURES             Location/Qualifiers
     source          1..1392
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1357..1383
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tctagagcca ccatggcatc atcgacctcc ctcccagctc ctggctctcg gcctaagaag
       61 cctctaggca agatggctga ctggttcagg cagaccctgc tgaagaagcc caagaagagg
      121 cccaactccc cagaaagcac ctccagcgat gcttcacagc ctacctcaca ggacagccca
      181 ctacccccaa gcctcagctc agtcacgtct cccagcctgc cacccacaca tgcgagtgac
      241 agtggcagta gtcgctggag caaagaccct ggggcctcga agctccgggc ggttttggag
      301 aagttgaagc tcagccgcga tgatatctcc acggcggcgg ggatggtgaa aggggttgtg
      361 gaccacctgc tgctcagact gaagtgcgac tccgcgttca gaggcgtcgg gctgctgaac
      421 accgggagct actatgagca cgtgaagatt tctgcaccta atgaatttga tgtcatgttt
      481 aaactggaag tccccagaat tcaactagaa gaatattcca acactcgtgc atattacttt
      541 gtgaaattta aaagaaatcc gaaagaaaat cctctgagtc agtttttaga aggtgaaata
      601 ttatcagctt ctaagatgct gtcaaagttt aggaaaatca ttaaggaaga aattaacgac
      661 attaaagata cagatgtcat catgaagagg aaaagaggag ggagccctgc tgtaacactt
      721 cttattagtg aaaaaatatc tgtggatata accctggctt tggaatcaaa aagtagctgg
      781 cctgctagca cccaagaagg cctgcgcatt caaaactggc tttcagcaaa agttaggaag
      841 caactacgac taaagccatt ttaccttgta cccaagcatg caaaggaagg aaatggtttc
      901 caagaagaaa catggcggct atccttctct cacatcgaaa aggaaatttt gaacaatcat
      961 ggaaaatcta aaacgtgctg tgaaaacaaa gaagagaaat gttgcaggaa agattgttta
     1021 aaactaatga aatacctttt agaacagctg aaagaaaggt ttaaagacaa aaaacatctg
     1081 gataaattct cttcttatca tgtgaaaact gccttctttc acgtatgtac ccagaaccct
     1141 caagacagtc agtgggaccg caaagacctg ggcctctgct ttgataactg cgtgacatac
     1201 tttcttcagt gcctcaggac agaaaaactt gagaattatt ttattcctga attcaatcta
     1261 ttctctagca acttaattga caaaagaagt aaggaatttc tgacaaagca aattgaatat
     1321 gaaagaaaca atgagtttcc agtttttgat gaattttacc catacgatgt tccagattac
     1381 gcttgagtcg ac
//
