LOCUS       sequence-250865-        1084 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A CRISPR activation device with the necessary genes (dcas9 
            controlled by Ptet, pspF-Delta-HTH::lambda-N22plus controlled by 
            promoter J23106), and the reporter part (PpspA-LEA3B3 with 
            sfgfp::ASV)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Liu Y, Wan X, Wang B
  TITLE     Engineered CRISPRa enables programmable eukaryote-like gene 
            activation in bacteria.
  JOURNAL   Nat Commun. 2019 Aug 26;10(1):3693. doi: 10.1038/s41467-019-11479-0.
  PUBMED    31451697
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-26"; volume: "10(1):3693. doi"; pages: " 
            10.1038/s41467-019-11479-0"
COMMENT     Sequence Label: pLY55
FEATURES             Location/Qualifiers
     source          1..1084
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..43
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     CDS             1042..1053
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tactagagtt tacggctagc tcagtcctag gtatagtgct agctactaga gtcacacagg
       61 aaagtactag atggcagaat acaaagataa tttacttggt gaggcgaaca gctttctcga
      121 agtgctggaa caggtttcgc atctcgcacc gctggacaaa ccggtgctca tcatcggcga
      181 acgcggcacc ggtaaagagc tgattgccag ccgcctgcat tatctctcct cccgttggca
      241 agggccgttt atttccctta actgcgcggc gttaaatgaa aatctgctgg attccgaact
      301 gtttggtcac gaagcggggg cgtttaccgg tgcgcaaaaa cgtcatccag ggagatttga
      361 acgtgccgac ggcggtacgc tatttcttga tgaactcgct acggcaccga tgatggtgca
      421 ggagaaatta ttgcgcgtga ttgagtacgg tgaactggag cgcgttggcg gcagccaacc
      481 attgcaggtg aatgtgcggt tggtatgcgc gacgaatgcc gatctcccgg cgatggtcaa
      541 tgaaggcact tttcgcgctg acctgctcga ccgactggct tttgatgttg tacaactgcc
      601 accactgcgc gagcgcgaaa gcgacataat gttgatggca gaatactttg ccatccagat
      661 gtgtcgggaa atcaagctgc ctctgttccc ggggtttacg gagcgcgcca gagaaacatt
      721 gctgaattat cgttggccgg gaaatattcg tgaattgaaa aacgtggtgg aacgttcagt
      781 gtatcgccac ggcaccagcg attatccgct tgatgacatc attattgatc cctttaaacg
      841 gcgtccgcct gaagacgcta tcgccgtttc agaaaccacc tcgcttccaa cactgccgct
      901 ggatttacgt gagtttcaga tgcagcagga aaaagagttg ctgcaactca gtttgcaaat
      961 gaatgcacgc acacgccgcc gcgaacgtcg cgcagagaaa caggctcaat ggaaagcagc
     1021 aaattaatac tagagccaat tattgaaggc cgctaacgcg gccttttttt gtttctggtc
     1081 tccc
//
