LOCUS       sequence-250871-       12852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of HA-tagged human beta-actin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12852)
  AUTHORS   Toneff MJ, Du Z, Dong J, Huang J, Sinai P, Forman J, Hilsenbeck S, 
            Schiff R, Huang S, Li Y
  TITLE     Somatic expression of PyMT or activated ErbB2 induces 
            estrogen-independent mammary tumorigenesis.
  JOURNAL   Neoplasia. 2010 Sep;12(9):718-26.
  PUBMED    20824048
REFERENCE   2  (bases 1 to 12852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neoplasia."; date: "2010-09"; volume: "12(9)"; pages: "718-26"
COMMENT     Sequence Label: RCAS-HA-b-actin
FEATURES             Location/Qualifiers
     source          1..12852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4..30
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        1346..1607
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2197..2219)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2208..2224
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2232..2238,2239..2256,2257..2262))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(2305..2323)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2391..2495
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2496..2564,2565..3356)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2714..2733)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3527..4115
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4016..4035
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4269..4286
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4301..4441
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4367..4386)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4527..4549
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4543..4734)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        5577..5838
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 atgtacccat acgatgttcc agattacgct aagcttgaat tcagatctcg agctatggat
       61 gatgatatcg ccgcgctcgt cgtcgacaac ggctccggca tgtgcaaggc cggcttcgcg
      121 ggcgacgatg ccccccgggc cgtcttcccc tccatcgtgg ggcgccccag gcaccagggc
      181 gtgatggtgg gcatgggtca gaaggattcc tatgtgggcg acgaggccca gagcaagaga
      241 ggcatcctca ccctgaagta ccccatcgag cacggcatcg tcaccaactg ggacgacatg
      301 gagaaaatct ggcaccacac cttctacaat gagctgcgtg tggctcccga ggagcacccc
      361 gtgctgctga ccgaggcccc cctgaacccc aaggccaacc gcgagaagat gacccagatc
      421 atgtttgaga ccttcaacac cccagccatg tacgttgcta tccaggctgt gctatccctg
      481 tacgcctctg gccgtaccac tggcatcgtg atggactccg gtgacggggt cacccacact
      541 gtgcccatct acgaggggta tgccctcccc catgccatcc tgcgtctgga cctggctggc
      601 cgggacctga ctgactacct catgaagatc ctcaccgagc gcggctacag cttcaccacc
      661 acggccgagc gggaaatcgt gcgtgacatt aaggagaagc tgtgctacgt cgccctggac
      721 ttcgagcaag agatggccac ggctgcttcc agctcctccc tggagaagag ctacgagctg
      781 cctgacggcc aggtcatcac cattggcaat gagcggttcc gctgccctga ggcactcttc
      841 cagccttcct tcctgggcat ggagtcctgt ggcatccacg aaactacctt caactccatc
      901 atgaagtgtg acgtggacat ccgcaaagac ctgtacgcca acacagtgct gtctggcggc
      961 accaccatgt accctggcat tgccgacagg atgcagaagg agatcactgc cctggcaccc
     1021 agcacaatga agatcaagat cattgctcct cctgagcgca agtactccgt gtggatcggc
     1081 ggctccatcc tggcctcgct gtccaccttc cagcagatgt ggatcagcaa gcaggagtat
     1141 gacgagtccg gcccctccat cgtccaccgc aaatgcttct agggatcaaa cttaattaaa
     1201 tttaaatatc gatgcgatgt acgggccaga tatacgcgta tctgagggga ctagggtgtg
     1261 tttaggcgaa aagcggggct tcggttgtac gcggttagga gtcccctcag gatatagtag
     1321 tttcgctttt gcatagggag ggggaaatgt agtcttatgc aatactcttg tagtcttgca
     1381 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     1441 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     1501 catggattgg acgaaccact gaattccgca ttgcagagat attgtattta agtgcctagc
     1561 tcgatacaat aaacgccatt tgaccattca ccacattggt gtgcacctgg gttgatggcc
     1621 ggaccgttga ttccctgacg actacgagca cctgcatgaa gcagaaggct tcatttggtg
     1681 accccgacgt gatagttagg gaatagtggt cggccacaga cggcgtggcg atcctgtctc
     1741 catccgtctc gtctatcggg aggcgagttc gatgaccctg gtggaggggg ctgcggctta
     1801 gggaggcaga agctgagtac cgtcggaggg gatccacagg acgggtgtgg tcgccatgat
     1861 cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg
     1921 gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag
     1981 cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg
     2041 cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac
     2101 gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga
     2161 taaactaccg cattaaagct ccaagcttgg ctgtttcctg tgtgaaattg ttatccgctc
     2221 acaattccac acattatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     2281 gtgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     2341 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     2401 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     2461 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     2521 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     2581 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     2641 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     2701 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     2761 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     2821 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     2881 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     2941 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     3001 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
     3061 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     3121 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     3181 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     3241 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     3301 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     3361 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     3421 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     3481 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3541 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3601 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3661 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3721 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3781 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3841 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3901 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3961 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4021 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4081 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4141 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     4201 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     4261 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     4321 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     4381 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     4441 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     4501 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     4561 atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc
     4621 acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt
     4681 ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc
     4741 ctccgtgtaa ggaggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat
     4801 gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa
     4861 acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg
     4921 cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat
     4981 ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa
     5041 accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca
     5101 cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag
     5161 ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc
     5221 cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg
     5281 gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt
     5341 gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca
     5401 ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgatgcgatg tacgggccag
     5461 atatacgcgt atctgagggg actagggtgt gtttaggcga aaagcggggc ttcggttgta
     5521 cgcggttagg agtcccctca ggatatagta gtttcgcttt tgcataggga gggggaaatg
     5581 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     5641 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     5701 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattccgc
     5761 attgcagaga tattgtattt aagtgcctag ctcgatacaa taaacgccat ttgaccattc
     5821 accacattgg tgtgcacctg ggttgatggc cggaccgttg attccctgac gactacgagc
     5881 acctgcatga agcagaaggc ttcatttggt gaccccgacg tgatagttag ggaatagtgg
     5941 tcggccacag acggcgtggc gatcctgtct ccatccgtct cgtctatcgg gaggcgagtt
     6001 cgatgaccct ggtggagggg gctgcggctt agggaggcag aagctgagta ccgtcggagg
     6061 gagctccagg gcccggagcg actgacccct gccgagaact cagagggtcg tcggaagacg
     6121 gagagtgagc ccgacgacca ccccaggcac gtctttggtc ggcctgcgga tcaagcatgg
     6181 aagccgtcat taaggtgatt tcgtccgcgt gtaaaaccta ttgcgggaaa atctctcctt
     6241 ctaagaagga aataggggcc atgttgtccc tgttacaaaa ggaagggttg cttatgtctc
     6301 cctcagattt atattctccg gggtcctggg atcccatcac tgcggcgctc tcccagcggg
     6361 caatggtact tggaaaatcg ggagagttaa aaacctgggg attggttttg ggggcattga
     6421 aggcggctcg agaggaacag gttacatctg agcaagcaaa gttttggttg ggattagggg
     6481 gagggagggt ctctccccca ggtccggagt gcatcgagaa accagctacg gagcggcgaa
     6541 tcgacaaagg ggaggaggtg ggagaaacaa ctgtgcagcg agatgcgaag atggcgccag
     6601 aggaagcggc cacacctaaa accgttggca catcctgcta tcattgcgga acagctgttg
     6661 gctgcaattg cgccaccgcc acagcctcgg cccctcctcc cccttatgtg gggagtggtt
     6721 tgtatccttc cctggcgggg gtgggagagc agcagggcca gggagataac acgtctcggg
     6781 gggcggagca gccaagggag gagccagggc acgcgggtca ggcccctggg ccggccctga
     6841 ctgactgggc aagggtaagg gaggagcttg cgagtactgg tccgcccgtg gtggccatgc
     6901 ctgtagtgat taagacagag ggacccgcct ggacccctct ggagccaaaa ttgatcacaa
     6961 gactggctga tacggtcagg accaagggct tacgatcccc gatcactatg gcagaagtgg
     7021 aagcgctcat gtcctccccg ttgctgccgc atgacgtcac gaatctaatg agagtgattt
     7081 taggacctgc cccatatgcc ttatggatgg acgcttgggg agtccaactc cagacggtta
     7141 tagcggcagc cactcgcgac ccccgacacc cagcgaacgg tcaagggcgg ggggaacgga
     7201 ctaacttgga tcgattaaag ggcttagctg atgggatggt gggcaaccca cagggtcagg
     7261 ccgcattatt aagaccgggg gaattggttg ctattacggc gtcggctctc caggcgttta
     7321 gagaagttgc ccggctggcg gaacctgcag gtccatgggc ggacatcacg cagggaccat
     7381 ctgagtcctt tgttgatttt gccaatcggc ttataaaggc ggttgagggg tcagatctcc
     7441 cgccttccgc gcgggctccg gtgatcattg actgctttag gcagaagtca cagccagata
     7501 ttcagcagct tatacgggca gcaccctcca cgctgaccac cccaggagag ataatcaaat
     7561 atgtgctaga caggcagaag attgcccctc ttacggatca aggcatagcc gcggccatgt
     7621 cgtctgctat ccagccctta gttatggcag tagtcaatag agagagggat ggacaaactg
     7681 ggtcgggtgg tcgtgcccga gggctctgct acacttgtgg atccccggga cattatcagg
     7741 cacagtgccc gaaaaaacga aagtcaggaa acagccgtga gcgatgtcag ctgtgtgacg
     7801 ggatgggaca caacgctaaa cagtgtagga agcgggatgg caaccagggc caacgcccag
     7861 gaagaggtct ctcttcgggg ccgtggcccg gccctgagca gcctgccgtc tcgttagcga
     7921 tgacaatgga acataaagat cgccccttgg ttagggtcat tctgactaac actgggagtc
     7981 atccagtcaa acaacgttcg gtgtatatca ccgcgctgtt ggactccgga gcggacatca
     8041 ctattatttc ggaggaggat tggcctactg attggccggt ggtggacacc gcgaacccac
     8101 agatccatgg cataggaggg ggaattccca tgcgaaaatc ccgggatatg atagaggtgg
     8161 gggttattaa ccgagacggg tcgttggagc gacccctgct cctcttcccc gcagtcgcta
     8221 tggttagagg gagtatccta ggaagagatt gtctgcaggg cctagggctc cgcttgacaa
     8281 atttataggg agggccactg ttcttactgt tgcgctacat ctggctattc cgctcaaatg
     8341 gaagtcagac cgcacgcctg tgtggattga ccagtggccc cttcctgaag gtaaacttgt
     8401 agcgctaacg caattagtgg aaaaagaatt acagttagga catatagagc cctcacttag
     8461 ttgttggaac acacctgttt ttgtgatccg gaaggcttcc gggtcttatc gcttattgca
     8521 tgatttgcgc gctgttaacg ccaagcttgt tccttttggg gccgtccaac agggggcgcc
     8581 agttctctcc gcgctcccgc gtggctggcc cctgatggtc ctagacctca aggattgctt
     8641 cttttctatc cctcttgcgg aacaagatcg cgaagctttt gcatttacgc tcccctctgt
     8701 gaataaccag gcccccgctc gaagattcca atggaaggtc ttgccccaag ggatgacctg
     8761 ttctcccact atctgtcagt tggtagtggg tcaggtgctc gagcccttgc gactcaagca
     8821 cccagctctg cgcatgttgc attatatgga cgatcttttg ctagccgcct caagtcatga
     8881 tgggttggaa gcggcaggga aggaggttat cggtacattg gaaagagccg ggttcactat
     8941 ttcgccggat aagatccaga gggagcccgg agtacaatat cttgggtaca agttaggcag
     9001 tacgtatgta gcacccgtag gcttggtagc agaacccagg atagccacct tgtgggatgt
     9061 tcaaaagctg gtggggtcac ttcagtggct tcgcccagcg ttagggatcc cgccacgact
     9121 gatgggtccc ttttatgagc agttacgagg gtcagatcct aacgaggcga gggaatggaa
     9181 tctagacatg aaaatggcct ggagagagat cgtacagctt agcactactg ctgccttgga
     9241 acgatgggac cctgcccagc ctctggaagg agcggtcgct agatgtgaac agggggcaat
     9301 aggggtcctg ggacagggac tgtccacaca cccaaggcca tgtttgtggt tattctccac
     9361 ccaacccacc aaggcgttta ctgcttggtt agaagtgctc acccttttga ttactaagct
     9421 acgcgcttcg gcagtgcgaa cctttggcaa ggaggttgat atcctcctgt tgcctgcatg
     9481 cttccgggag gaccttccgc tcccggaggg gatcctgtta gcacttaggg ggtttgcagg
     9541 aaaaatcagg agtagtgaca cgccatctat ttttgacatt gcgcgtccac tgcatgtttc
     9601 tctgaaagtg agggttaccg accaccctgt gccgggaccc actgtcttta ccgacgcctc
     9661 ctcaagcacc cataaagggg tggtagtctg gagggagggc ccaaggtggg agataaaaga
     9721 aatagttgat ttgggggcaa gtgtacaaca actggaggca cgcgctgtgg ccatggcact
     9781 tctgctgtgg ccgacaacgc ccactaatgt agtgactgac tctgcgtttg ttgcgaaaat
     9841 gttactcaag atgggacagg agggagtccc gtctacagcg gcagctttta ttttagagga
     9901 tgcgttaagc caaaggtcag ccatggccgc cgttctccac gtgcggagtc attctgaagt
     9961 gccagggttt ttcacagaag gaaatgacgt ggcagatagc caagccacct ttcaagcgta
    10021 tcccttgaga gaggctaaag atcttcatac cgctctccat attggacccc gcgcgctatc
    10081 caaagcgtgt aatatatcta tgcagcaggc tagggaggtt gttcagacct gcccgcattg
    10141 taattcagcc cctgcgttgg aggccggggt aaaccctagg ggtttgggac ccctacagat
    10201 atggcagaca gactttacgc ttgagcctag aatggctccc cgttcctggc tcgctgttac
    10261 tgtggacacc gcctcatcag cgatagtcgt aactcagcat ggccgtgtta catcggttgc
    10321 tgcacaacat cattgggcca cggctatcgc cgttttggga agaccaaagg ccataaaaac
    10381 agataacggg tcctgcttca cgtctaaatc cacgcgggag tggctcgcga gatgggggat
    10441 agcacacacc accgggattc cgggaaattc ccagggtcaa gctatggtag agcgggccaa
    10501 ccggctcctg aaagataaga tccgtgtgct tgcggaaggg gacggcttta tgaaaagaat
    10561 ccccgccagc aaacaggggg aactactagc caaagcaatg tatgccctca atcactttga
    10621 gcgtggtgaa aacacaaaaa caccggtaca aaaacactgg agacctaccg ttcttacaga
    10681 aggacccccg gttaaaatac gaatagagac aggggagtgg gaaaaaggat ggaacgtgct
    10741 ggtctgggga cgaggttatg ccgctgtgaa aaacagggac actgataagg ttatttgggt
    10801 accctctcgg aaagttaaac cggatgtcac ccaaaaggat gaggtgacta agaaagatga
    10861 ggcgagccct ctttttgcag gcatttctga ctggataccc tgggaagacg agcaagaagg
    10921 actccaagga gaaaccgcta gcaacaagca agaaagaccc ggagaagaca cccttgctgc
    10981 caacgagagt taattatatt ctcattattg gtgtcctggt cttgtgtgag gttacggggg
    11041 taagagctga tgtccactta ctcgagcagc cagggaacct ttggattaca tgggccaacc
    11101 gtacaggcca aacggatttt tgcctctcta cacagtcagc cacctcccct tttcaaacat
    11161 gtttgatagg tatcccgtcc cctatttccg agggtgattt taagggatat gtttctgata
    11221 caaattgcac caccttggga actgatcggt tagtctcgtc agccgacttt actggcggac
    11281 ctgacaacag taccaccctc acttatcgga aggtctcatg cttgttgtta aagctgaatg
    11341 tctctatgtg ggatgagcca cctgaactac agctgttagg ttcccagtct ctccctaaca
    11401 ttactaatat tgctcagatt tccggtataa ccgggggatg cgtaggcttc agaccacaag
    11461 gggttccttg gtatctaggt tggtctagac aggaggccac gcggtttctc cttagacacc
    11521 cctctttctc taaatccacg gaaccgttta cggtggtgac agcggatagg cacaatcttt
    11581 ttatggggag tgagtactgc ggtgcatatg gctacagatt ttggaacatg tataactgct
    11641 cacaggtggg gcggcagtac cgctgtggta atgcgcgcag cccccgcccg ggtcttcctg
    11701 aaatccagtg tacaaggaga ggaggcaaat gggttaatca atcacaggaa attaatgagt
    11761 cggagccgtt cagctttacg gtgaactgta cagctagtag tttgggtaat gccagtgggt
    11821 gttgcggaaa agcaggcacg attctcccgg gaaagtgggt cgacagcaca caaggtagtt
    11881 tcaccaaacc aaaagcgcta ccacccgcaa ttttcctcat ttgtggggat cgcgcatggc
    11941 aaggaattcc cagtcgtccg gtagggggcc cctgctattt aggcaagctt accatgttag
    12001 cacctaagca tacagatatt ctcaaggtgc ttgtcaattc atcgcggaca ggtataagac
    12061 gtaaacgaag cacctcacac ctggatgata catgctcaga tgaagtgcag ctttggggtc
    12121 ctacagcaag aatctttgca tctatcctag ccccgggggt agcagctgcg caagccttaa
    12181 gagaaattga gagactagcc tgttggtccg ttaaacaggc taacttgaca acatcactcc
    12241 tcggggactt attggatgat gtcacgagta ttcgacacgc ggtcctgcag aaccgagcgg
    12301 ctattgactt cttgctccta gctcacggcc atggctgtga ggacgttgcc ggaatgtgct
    12361 gtttcaattt gagtgatcac agtgagtcta tacagaagaa gttccagcta atgaaggaac
    12421 atgtcaataa gatcggcgtg gacagcgacc caattggaag ttggctgcga ggactattcg
    12481 ggggaatagg agaatgggcc gttcatttgc tgaaaggact gcttttgggg cttgtagtta
    12541 ttttgttgct agtagtgtgc ctgccttgcc ttttgcaaat cgtatgcggt aacatcagaa
    12601 agatgattaa taactccatc agctaccaca cggaatataa gaagctgcaa aaggcctgtg
    12661 ggcagcctga aagcagaata gtataaggca gtacatgggt ggtggtatag cgcttgcgag
    12721 tccatcgagg aaggcaggaa agacagctat tggtaattgt gaaatacgct tttgtctgtg
    12781 tgctgcagga gctgagctga ctctgctggt ggcctcgcgt accactgtgg catcggcggc
    12841 cgcgtttgat cc
//
