LOCUS       sequence-250872-        1614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human cGAS-HA with C396/397A point mutations; Puromycin 
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Barnett KC, Coronas-Serna JM, Zhou W, Ernandes MJ, Cao A, Kranzusch 
            PJ, Kagan JC
  TITLE     Phosphoinositide Interactions Position cGAS at the Plasma Membrane 
            to Ensure Efficient Distinction between Self- and Viral DNA.
  JOURNAL   Cell. 2019 Mar 7;176(6):1432-1446.e11. doi: 
            10.1016/j.cell.2019.01.049. Epub 2019 Feb 28.
  PUBMED    30827685
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.01.049"; journalName: "Cell"; date: "2019-03-7-
            7"; volume: "176"; issue: "6"; pages: "1432-1446.e11"
COMMENT     Sequence Label: pLenti-CMV-cGAS C396/7A-HA
FEATURES             Location/Qualifiers
     source          1..1614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1579..1605
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tctagagcca ccatgcagcc ttggcacgga aaggccatgc agagagcttc cgaggccgga
       61 gccactgccc ccaaggcttc cgcacggaat gccaggggcg ccccgatgga tcccaccgag
      121 tctccggctg cccccgaggc cgccctgcct aaggcgggaa agttcggccc cgccaggaag
      181 tcgggatccc ggcagaaaaa gagcgccccg gacacccagg agaggccgcc cgtccgcgca
      241 actggggccc gcgccaaaaa ggcccctcag cgcgcccagg acacgcagcc gtctgacgcc
      301 accagcgccc ctggggcaga ggggctggag cctcctgcgg ctcgggagcc ggctctttcc
      361 agggctggtt cttgccgcca gaggggcgcg cgctgctcca cgaagccaag acctccgccc
      421 gggccctggg acgtgcccag ccccggcctg ccggtctcgg cccccattct cgtacggagg
      481 gatgcggcgc ctggggcctc gaagctccgg gcggttttgg agaagttgaa gctcagccgc
      541 gatgatatct ccacggcggc ggggatggtg aaaggggttg tggaccacct gctgctcaga
      601 ctgaagtgcg actccgcgtt cagaggcgtc gggctgctga acaccgggag ctactatgag
      661 cacgtgaaga tttctgcacc taatgaattt gatgtcatgt ttaaactgga agtccccaga
      721 attcaactag aagaatattc caacactcgt gcatattact ttgtgaaatt taaaagaaat
      781 ccgaaagaaa atcctctgag tcagttttta gaaggtgaaa tattatcagc ttctaagatg
      841 ctgtcaaagt ttaggaaaat cattaaggaa gaaattaacg acattaaaga tacagatgtc
      901 atcatgaaga ggaaaagagg agggagccct gctgtaacac ttcttattag tgaaaaaata
      961 tctgtggata taaccctggc tttggaatca aaaagtagct ggcctgctag cacccaagaa
     1021 ggcctgcgca ttcaaaactg gctttcagca aaagttagga agcaactacg actaaagcca
     1081 ttttaccttg tacccaagca tgcaaaggaa ggaaatggtt tccaagaaga aacatggcgg
     1141 ctatccttct ctcacatcga aaaggaaatt ttgaacaatc atggaaaatc taaaacggcc
     1201 gctgaaaaca aagaagagaa atgttgcagg aaagattgtt taaaactaat gaaatacctt
     1261 ttagaacagc tgaaagaaag gtttaaagac aaaaaacatc tggataaatt ctcttcttat
     1321 catgtgaaaa ctgccttctt tcacgtatgt acccagaacc ctcaagacag tcagtgggac
     1381 cgcaaagacc tgggcctctg ctttgataac tgcgtgacat actttcttca gtgcctcagg
     1441 acagaaaaac ttgagaatta ttttattcct gaattcaatc tattctctag caacttaatt
     1501 gacaaaagaa gtaaggaatt tctgacaaag caaattgaat atgaaagaaa caatgagttt
     1561 ccagtttttg atgaatttta cccatacgat gttccagatt acgcttgagt cgac
//
