LOCUS       sequence-250873-         489 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses murine cGAS N terminus(aa1-147)-HA; Puromycin selection 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Barnett KC, Coronas-Serna JM, Zhou W, Ernandes MJ, Cao A, Kranzusch 
            PJ, Kagan JC
  TITLE     Phosphoinositide Interactions Position cGAS at the Plasma Membrane 
            to Ensure Efficient Distinction between Self- and Viral DNA.
  JOURNAL   Cell. 2019 Mar 7;176(6):1432-1446.e11. doi: 
            10.1016/j.cell.2019.01.049. Epub 2019 Feb 28.
  PUBMED    30827685
REFERENCE   2  (bases 1 to 489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.01.049"; journalName: "Cell"; date: "2019-03-7-
            7"; volume: "176"; issue: "6"; pages: "1432-1446.e11"
COMMENT     Sequence Label: pLenti-CMV-cGAS-N-HA (murine)
FEATURES             Location/Qualifiers
     source          1..489
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             454..480
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tctagagcca ccatggaaga tccgcgtaga aggacgacgg cgccacgcgc taagaagccg
       61 tccgcgaagc gcgccccgac gcagcccagc aggaccaggg cccacgcgga aagctgcggc
      121 ccgcaaaggg gggctcgatc gcggcgggcg gagcgtgacg gggacaccac ggagaagcca
      181 cgtgccccag ggccccgagt gcatccagca agggccactg agctcaccaa agatgcacag
      241 ccctcggcca tggacgcggc aggagccacc gcgcggcctg ccgtccgggt gccccagcag
      301 caggccatcc tggatccgga gctgcccgcc gtacgggagc cccagccgcc cgcggatccc
      361 gaggcgcgga aagtcgtaag gggacctagc cacagaaggg gcgcgcgctc caccgggcag
      421 cccagagcgc cgcgagggtc caggaaggaa ccgtacccat acgatgttcc agattacgct
      481 tgagtcgac
//
