LOCUS       sequence-250874-       13977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of Flag tagged human oncogenic BRAFV600E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13977)
  AUTHORS   Bu W, Liu Z, Jiang W, Nagi C, Huang S, Edwards DP, Jo E, Mo Q, 
            Creighton CJ, Hilsenbeck SG, Leavitt AD, Lewis MT, Wong STC, Li Y
  TITLE     Mammary Precancerous Stem and Non-Stem Cells Evolve into Cancers of 
            Distinct Subtypes.
  JOURNAL   Cancer Res. 2019 Jan 1;79(1):61-71. doi: 
            10.1158/0008-5472.CAN-18-1087. Epub 2018 Nov 6.
  PUBMED    30401712
REFERENCE   2  (bases 1 to 13977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-18-1087"; journalName: "Cancer Res"; date: 
            "2019-01-1- 1"; volume: "79"; issue: "1"; pages: "61-71"
COMMENT     Sequence Label: RCAS-Flag-BRAFV600E
FEATURES             Location/Qualifiers
     source          1..13977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..24
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        2461..2722
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(3312..3334)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3323..3339
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3347..3353,3354..3371,3372..3377))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(3420..3438)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3506..3610
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3611..3679,3680..4471)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3829..3848)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4642..5230
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5131..5150
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5384..5401
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5416..5556
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5482..5501)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5642..5664
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5658..5849)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        6692..6953
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gattataaag atgacgacga taaggcggcg ctgagcggtg gcggtggtgg cggcgcggag
       61 ccgggccagg ctctgttcaa cggggacatg gagcccgagg ccggcgccgg cgccggcgcc
      121 gcggcctctt cggctgcgga ccctgccatt ccggaggagg tgtggaatat caaacaaatg
      181 attaagttga cacaggaaca tatagaggcc ctattggaca aatttggtgg ggagcataat
      241 ccaccatcaa tatatctgga ggcctatgaa gaatacacca gcaagctaga tgcactccaa
      301 caaagagaac aacagttatt ggaatctctg gggaacggaa ctgatttttc tgtttctagc
      361 tctgcatcaa tggataccgt tacatcttct tcctcttcta gcctttcagt gctaccttca
      421 tctctttcag tttttcaaaa tcccacagat gtggcacgga gcaaccccaa gtcaccacaa
      481 aaacctatcg ttagagtctt cctgcccaac aaacagagga cagtggtacc tgcaaggtgt
      541 ggagttacag tccgagacag tctaaagaaa gcactgatga tgagaggtct aatcccagag
      601 tgctgtgctg tttacagaat tcaggatgga gagaagaaac caattggttg ggacactgat
      661 atttcctggc ttactggaga agaattgcat gtggaagtgt tggagaatgt tccacttaca
      721 acacacaact ttgtacgaaa aacgtttttc accttagcat tttgtgactt ttgtcgaaag
      781 ctgcttttcc agggtttccg ctgtcaaaca tgtggttata aatttcacca gcgttgtagt
      841 acagaagttc cactgatgtg tgttaattat gaccaacttg atttgctgtt tgtctccaag
      901 ttctttgaac accacccaat accacaggaa gaggcgtcct tagcagagac tgccctaaca
      961 tctggatcat ccccttccgc acccgcctcg gactctattg ggccccaaat tctcaccagt
     1021 ccgtctcctt caaaatccat tccaattcca cagcccttcc gaccagcaga tgaagatcat
     1081 cgaaatcaat ttgggcaacg agaccgatcc tcatcagctc ccaatgtgca tataaacaca
     1141 atagaacctg tcaatattga tgacttgatt agagaccaag gatttcgtgg tgatggagga
     1201 tcaaccacag gtttgtctgc taccccccct gcctcattac ctggctcact aactaacgtg
     1261 aaagccttac agaaatctcc aggacctcag cgagaaagga agtcatcttc atcctcagaa
     1321 gacaggaatc gaatgaaaac acttggtaga cgggactcga gtgatgattg ggagattcct
     1381 gatgggcaga ttacagtggg acaaagaatt ggatctggat catttggaac agtctacaag
     1441 ggaaagtggc atggtgatgt ggcagtgaaa atgttgaatg tgacagcacc tacacctcag
     1501 cagttacaag ccttcaaaaa tgaagtagga gtactcagga aaacacgaca tgtgaatatc
     1561 ctactcttca tgggctattc cacaaagcca caactggcta ttgttaccca gtggtgtgag
     1621 ggctccagct tgtatcacca tctccatatc attgagacca aatttgagat gatcaaactt
     1681 atagatattg cacgacagac tgcacagggc atggattact tacacgccaa gtcaatcatc
     1741 cacagagacc tcaagagtaa taatatattt cttcatgaag acctcacagt aaaaataggt
     1801 gattttggtc tagctacaga gaaatctcga tggagtgggt cccatcagtt tgaacagttg
     1861 tctggatcca ttttgtggat ggcaccagaa gtcatcagaa tgcaagataa aaatccatac
     1921 agctttcagt cagatgtata tgcatttgga attgttctgt atgaattgat gactggacag
     1981 ttaccttatt caaacatcaa caacagggac cagataattt ttatggtggg acgaggatac
     2041 ctgtctccag atctcagtaa ggtacggagt aactgtccaa aagccatgaa gagattaatg
     2101 gcagagtgcc tcaaaaagaa aagagatgag agaccactct ttccccaaat tctcgcctct
     2161 attgagctgc tggcccgctc attgccaaaa attcaccgca gtgcatcaga accctccttg
     2221 aatcgggctg gtttccaaac agaggatttt agtctatatg cttgtgcttc tccaaaaaca
     2281 cccatccagg cagggggata tggtgcgttt cctgtccact gaatcgatgc gatgtacggg
     2341 ccagatatac gcgtatctga ggggactagg gtgtgtttag gcgaaaagcg gggcttcggt
     2401 tgtacgcggt taggagtccc ctcaggatat agtagtttcg cttttgcata gggaggggga
     2461 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     2521 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     2581 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     2641 ccgcattgca gagatattgt atttaagtgc ctagctcgat acaataaacg ccatttgacc
     2701 attcaccaca ttggtgtgca cctgggttga tggccggacc gttgattccc tgacgactac
     2761 gagcacctgc atgaagcaga aggcttcatt tggtgacccc gacgtgatag ttagggaata
     2821 gtggtcggcc acagacggcg tggcgatcct gtctccatcc gtctcgtcta tcgggaggcg
     2881 agttcgatga ccctggtgga gggggctgcg gcttagggag gcagaagctg agtaccgtcg
     2941 gaggggatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt
     3001 agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt
     3061 gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg
     3121 ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg
     3181 cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata
     3241 cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagctccaag
     3301 cttggctgtt tcctgtgtga aattgttatc cgctcacaat tccacacatt atacgagccg
     3361 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag aattcttgaa gacgaaaggg
     3421 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     3481 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3541 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3601 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     3661 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     3721 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     3781 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     3841 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     3901 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     3961 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     4021 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     4081 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     4141 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     4201 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     4261 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     4321 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     4381 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     4441 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     4501 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4561 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4621 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4681 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4741 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     4801 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4861 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4921 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4981 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5041 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5101 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5161 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     5221 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     5281 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     5341 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     5401 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     5461 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     5521 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     5581 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     5641 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg
     5701 cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg
     5761 tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg
     5821 ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggagg atttctgttc
     5881 atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat
     5941 gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg
     6001 gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt
     6061 ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct
     6121 gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct
     6181 caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca
     6241 ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg
     6301 atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg
     6361 gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc
     6421 cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg
     6481 cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga
     6541 caaggtatag ggcggcgatg cgatgtacgg gccagatata cgcgtatctg aggggactag
     6601 ggtgtgttta ggcgaaaagc ggggcttcgg ttgtacgcgg ttaggagtcc cctcaggata
     6661 tagtagtttc gcttttgcat agggaggggg aaatgtagtc ttatgcaata ctcttgtagt
     6721 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     6781 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     6841 gtctgacatg gattggacga accactgaat tccgcattgc agagatattg tatttaagtg
     6901 cctagctcga tacaataaac gccatttgac cattcaccac attggtgtgc acctgggttg
     6961 atggccggac cgttgattcc ctgacgacta cgagcacctg catgaagcag aaggcttcat
     7021 ttggtgaccc cgacgtgata gttagggaat agtggtcggc cacagacggc gtggcgatcc
     7081 tgtctccatc cgtctcgtct atcgggaggc gagttcgatg accctggtgg agggggctgc
     7141 ggcttaggga ggcagaagct gagtaccgtc ggagggagct ccagggcccg gagcgactga
     7201 cccctgccga gaactcagag ggtcgtcgga agacggagag tgagcccgac gaccacccca
     7261 ggcacgtctt tggtcggcct gcggatcaag catggaagcc gtcattaagg tgatttcgtc
     7321 cgcgtgtaaa acctattgcg ggaaaatctc tccttctaag aaggaaatag gggccatgtt
     7381 gtccctgtta caaaaggaag ggttgcttat gtctccctca gatttatatt ctccggggtc
     7441 ctgggatccc atcactgcgg cgctctccca gcgggcaatg gtacttggaa aatcgggaga
     7501 gttaaaaacc tggggattgg ttttgggggc attgaaggcg gctcgagagg aacaggttac
     7561 atctgagcaa gcaaagtttt ggttgggatt agggggaggg agggtctctc ccccaggtcc
     7621 ggagtgcatc gagaaaccag ctacggagcg gcgaatcgac aaaggggagg aggtgggaga
     7681 aacaactgtg cagcgagatg cgaagatggc gccagaggaa gcggccacac ctaaaaccgt
     7741 tggcacatcc tgctatcatt gcggaacagc tgttggctgc aattgcgcca ccgccacagc
     7801 ctcggcccct cctccccctt atgtggggag tggtttgtat ccttccctgg cgggggtggg
     7861 agagcagcag ggccagggag ataacacgtc tcggggggcg gagcagccaa gggaggagcc
     7921 agggcacgcg ggtcaggccc ctgggccggc cctgactgac tgggcaaggg taagggagga
     7981 gcttgcgagt actggtccgc ccgtggtggc catgcctgta gtgattaaga cagagggacc
     8041 cgcctggacc cctctggagc caaaattgat cacaagactg gctgatacgg tcaggaccaa
     8101 gggcttacga tccccgatca ctatggcaga agtggaagcg ctcatgtcct ccccgttgct
     8161 gccgcatgac gtcacgaatc taatgagagt gattttagga cctgccccat atgccttatg
     8221 gatggacgct tggggagtcc aactccagac ggttatagcg gcagccactc gcgacccccg
     8281 acacccagcg aacggtcaag ggcgggggga acggactaac ttggatcgat taaagggctt
     8341 agctgatggg atggtgggca acccacaggg tcaggccgca ttattaagac cgggggaatt
     8401 ggttgctatt acggcgtcgg ctctccaggc gtttagagaa gttgcccggc tggcggaacc
     8461 tgcaggtcca tgggcggaca tcacgcaggg accatctgag tcctttgttg attttgccaa
     8521 tcggcttata aaggcggttg aggggtcaga tctcccgcct tccgcgcggg ctccggtgat
     8581 cattgactgc tttaggcaga agtcacagcc agatattcag cagcttatac gggcagcacc
     8641 ctccacgctg accaccccag gagagataat caaatatgtg ctagacaggc agaagattgc
     8701 ccctcttacg gatcaaggca tagccgcggc catgtcgtct gctatccagc ccttagttat
     8761 ggcagtagtc aatagagaga gggatggaca aactgggtcg ggtggtcgtg cccgagggct
     8821 ctgctacact tgtggatccc cgggacatta tcaggcacag tgcccgaaaa aacgaaagtc
     8881 aggaaacagc cgtgagcgat gtcagctgtg tgacgggatg ggacacaacg ctaaacagtg
     8941 taggaagcgg gatggcaacc agggccaacg cccaggaaga ggtctctctt cggggccgtg
     9001 gcccggccct gagcagcctg ccgtctcgtt agcgatgaca atggaacata aagatcgccc
     9061 cttggttagg gtcattctga ctaacactgg gagtcatcca gtcaaacaac gttcggtgta
     9121 tatcaccgcg ctgttggact ccggagcgga catcactatt atttcggagg aggattggcc
     9181 tactgattgg ccggtggtgg acaccgcgaa cccacagatc catggcatag gagggggaat
     9241 tcccatgcga aaatcccggg atatgataga ggtgggggtt attaaccgag acgggtcgtt
     9301 ggagcgaccc ctgctcctct tccccgcagt cgctatggtt agagggagta tcctaggaag
     9361 agattgtctg cagggcctag ggctccgctt gacaaattta tagggagggc cactgttctt
     9421 actgttgcgc tacatctggc tattccgctc aaatggaagt cagaccgcac gcctgtgtgg
     9481 attgaccagt ggccccttcc tgaaggtaaa cttgtagcgc taacgcaatt agtggaaaaa
     9541 gaattacagt taggacatat agagccctca cttagttgtt ggaacacacc tgtttttgtg
     9601 atccggaagg cttccgggtc ttatcgctta ttgcatgatt tgcgcgctgt taacgccaag
     9661 cttgttcctt ttggggccgt ccaacagggg gcgccagttc tctccgcgct cccgcgtggc
     9721 tggcccctga tggtcctaga cctcaaggat tgcttctttt ctatccctct tgcggaacaa
     9781 gatcgcgaag cttttgcatt tacgctcccc tctgtgaata accaggcccc cgctcgaaga
     9841 ttccaatgga aggtcttgcc ccaagggatg acctgttctc ccactatctg tcagttggta
     9901 gtgggtcagg tgctcgagcc cttgcgactc aagcacccag ctctgcgcat gttgcattat
     9961 atggacgatc ttttgctagc cgcctcaagt catgatgggt tggaagcggc agggaaggag
    10021 gttatcggta cattggaaag agccgggttc actatttcgc cggataagat ccagagggag
    10081 cccggagtac aatatcttgg gtacaagtta ggcagtacgt atgtagcacc cgtaggcttg
    10141 gtagcagaac ccaggatagc caccttgtgg gatgttcaaa agctggtggg gtcacttcag
    10201 tggcttcgcc cagcgttagg gatcccgcca cgactgatgg gtccctttta tgagcagtta
    10261 cgagggtcag atcctaacga ggcgagggaa tggaatctag acatgaaaat ggcctggaga
    10321 gagatcgtac agcttagcac tactgctgcc ttggaacgat gggaccctgc ccagcctctg
    10381 gaaggagcgg tcgctagatg tgaacagggg gcaatagggg tcctgggaca gggactgtcc
    10441 acacacccaa ggccatgttt gtggttattc tccacccaac ccaccaaggc gtttactgct
    10501 tggttagaag tgctcaccct tttgattact aagctacgcg cttcggcagt gcgaaccttt
    10561 ggcaaggagg ttgatatcct cctgttgcct gcatgcttcc gggaggacct tccgctcccg
    10621 gaggggatcc tgttagcact tagggggttt gcaggaaaaa tcaggagtag tgacacgcca
    10681 tctatttttg acattgcgcg tccactgcat gtttctctga aagtgagggt taccgaccac
    10741 cctgtgccgg gacccactgt ctttaccgac gcctcctcaa gcacccataa aggggtggta
    10801 gtctggaggg agggcccaag gtgggagata aaagaaatag ttgatttggg ggcaagtgta
    10861 caacaactgg aggcacgcgc tgtggccatg gcacttctgc tgtggccgac aacgcccact
    10921 aatgtagtga ctgactctgc gtttgttgcg aaaatgttac tcaagatggg acaggaggga
    10981 gtcccgtcta cagcggcagc ttttatttta gaggatgcgt taagccaaag gtcagccatg
    11041 gccgccgttc tccacgtgcg gagtcattct gaagtgccag ggtttttcac agaaggaaat
    11101 gacgtggcag atagccaagc cacctttcaa gcgtatccct tgagagaggc taaagatctt
    11161 cataccgctc tccatattgg accccgcgcg ctatccaaag cgtgtaatat atctatgcag
    11221 caggctaggg aggttgttca gacctgcccg cattgtaatt cagcccctgc gttggaggcc
    11281 ggggtaaacc ctaggggttt gggaccccta cagatatggc agacagactt tacgcttgag
    11341 cctagaatgg ctccccgttc ctggctcgct gttactgtgg acaccgcctc atcagcgata
    11401 gtcgtaactc agcatggccg tgttacatcg gttgctgcac aacatcattg ggccacggct
    11461 atcgccgttt tgggaagacc aaaggccata aaaacagata acgggtcctg cttcacgtct
    11521 aaatccacgc gggagtggct cgcgagatgg gggatagcac acaccaccgg gattccggga
    11581 aattcccagg gtcaagctat ggtagagcgg gccaaccggc tcctgaaaga taagatccgt
    11641 gtgcttgcgg aaggggacgg ctttatgaaa agaatccccg ccagcaaaca gggggaacta
    11701 ctagccaaag caatgtatgc cctcaatcac tttgagcgtg gtgaaaacac aaaaacaccg
    11761 gtacaaaaac actggagacc taccgttctt acagaaggac ccccggttaa aatacgaata
    11821 gagacagggg agtgggaaaa aggatggaac gtgctggtct ggggacgagg ttatgccgct
    11881 gtgaaaaaca gggacactga taaggttatt tgggtaccct ctcggaaagt taaaccggat
    11941 gtcacccaaa aggatgaggt gactaagaaa gatgaggcga gccctctttt tgcaggcatt
    12001 tctgactgga taccctggga agacgagcaa gaaggactcc aaggagaaac cgctagcaac
    12061 aagcaagaaa gacccggaga agacaccctt gctgccaacg agagttaatt atattctcat
    12121 tattggtgtc ctggtcttgt gtgaggttac gggggtaaga gctgatgtcc acttactcga
    12181 gcagccaggg aacctttgga ttacatgggc caaccgtaca ggccaaacgg atttttgcct
    12241 ctctacacag tcagccacct ccccttttca aacatgtttg ataggtatcc cgtcccctat
    12301 ttccgagggt gattttaagg gatatgtttc tgatacaaat tgcaccacct tgggaactga
    12361 tcggttagtc tcgtcagccg actttactgg cggacctgac aacagtacca ccctcactta
    12421 tcggaaggtc tcatgcttgt tgttaaagct gaatgtctct atgtgggatg agccacctga
    12481 actacagctg ttaggttccc agtctctccc taacattact aatattgctc agatttccgg
    12541 tataaccggg ggatgcgtag gcttcagacc acaaggggtt ccttggtatc taggttggtc
    12601 tagacaggag gccacgcggt ttctccttag acacccctct ttctctaaat ccacggaacc
    12661 gtttacggtg gtgacagcgg ataggcacaa tctttttatg gggagtgagt actgcggtgc
    12721 atatggctac agattttgga acatgtataa ctgctcacag gtggggcggc agtaccgctg
    12781 tggtaatgcg cgcagccccc gcccgggtct tcctgaaatc cagtgtacaa ggagaggagg
    12841 caaatgggtt aatcaatcac aggaaattaa tgagtcggag ccgttcagct ttacggtgaa
    12901 ctgtacagct agtagtttgg gtaatgccag tgggtgttgc ggaaaagcag gcacgattct
    12961 cccgggaaag tgggtcgaca gcacacaagg tagtttcacc aaaccaaaag cgctaccacc
    13021 cgcaattttc ctcatttgtg gggatcgcgc atggcaagga attcccagtc gtccggtagg
    13081 gggcccctgc tatttaggca agcttaccat gttagcacct aagcatacag atattctcaa
    13141 ggtgcttgtc aattcatcgc ggacaggtat aagacgtaaa cgaagcacct cacacctgga
    13201 tgatacatgc tcagatgaag tgcagctttg gggtcctaca gcaagaatct ttgcatctat
    13261 cctagccccg ggggtagcag ctgcgcaagc cttaagagaa attgagagac tagcctgttg
    13321 gtccgttaaa caggctaact tgacaacatc actcctcggg gacttattgg atgatgtcac
    13381 gagtattcga cacgcggtcc tgcagaaccg agcggctatt gacttcttgc tcctagctca
    13441 cggccatggc tgtgaggacg ttgccggaat gtgctgtttc aatttgagtg atcacagtga
    13501 gtctatacag aagaagttcc agctaatgaa ggaacatgtc aataagatcg gcgtggacag
    13561 cgacccaatt ggaagttggc tgcgaggact attcggggga ataggagaat gggccgttca
    13621 tttgctgaaa ggactgcttt tggggcttgt agttattttg ttgctagtag tgtgcctgcc
    13681 ttgccttttg caaatcgtat gcggtaacat cagaaagatg attaataact ccatcagcta
    13741 ccacacggaa tataagaagc tgcaaaaggc ctgtgggcag cctgaaagca gaatagtata
    13801 aggcagtaca tgggtggtgg tatagcgctt gcgagtccat cgaggaaggc aggaaagaca
    13861 gctattggta attgtgaaat acgcttttgt ctgtgtgctg caggagctga gctgactctg
    13921 ctggtggcct cgcgtaccac tgtggcatcg gcggccgcgt ttaaacttaa ttaaatg
//
