LOCUS       sequence-250875-        1131 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses murine cGAS-Delta-N (aa148-507)-HA; Puromycin selection 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Barnett KC, Coronas-Serna JM, Zhou W, Ernandes MJ, Cao A, Kranzusch 
            PJ, Kagan JC
  TITLE     Phosphoinositide Interactions Position cGAS at the Plasma Membrane 
            to Ensure Efficient Distinction between Self- and Viral DNA.
  JOURNAL   Cell. 2019 Mar 7;176(6):1432-1446.e11. doi: 
            10.1016/j.cell.2019.01.049. Epub 2019 Feb 28.
  PUBMED    30827685
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.01.049"; journalName: "Cell"; date: "2019-03-7-
            7"; volume: "176"; issue: "6"; pages: "1432-1446.e11"
COMMENT     Sequence Label: pLenti-CMV-cGAS-Delta-N-HA (murine)
FEATURES             Location/Qualifiers
     source          1..1131
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1096..1122
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tctagagcca ccatggacaa gctaaagaag gtgctggaca aattgagatt gaaacgcaaa
       61 gatatctcgg aggcggccga gacggtgaat aaagttgtgg aacgcctgct gcgcagaatg
      121 cagaaacggg agtcggagtt caaaggtgtg gagcagctga acactggcag ctactatgaa
      181 catgtgaaga tttctgctcc taatgaattt gatgttatgt ttaaactgga agtccccagg
      241 attgagctac aagaatatta tgaaacaggt gctttctatc ttgtgaaatt caaaagaatt
      301 ccacgaggaa atccgctgag tcatttctta gaaggggaag tattatcagc taccaagatg
      361 ctgtcaaagt ttaggaaaat cattaaagaa gaagttaaag aaatcaaaga tatagatgtc
      421 agtgtggaga aggaaaaacc aggaagccct gctgtaacac ttcttatcag gaaccctgaa
      481 gaaatctctg tggatataat tctggctttg gagtcaaaag gcagctggcc tattagtacc
      541 aaagaaggac tacctattca aggctggctg ggcacaaaag tgaggaccaa tctaagacga
      601 gagccgtttt atctcgtacc caagaatgca aaggatggaa atagttttca aggagagacc
      661 tggcgcctct ctttctctca cactgaaaag tacattttga ataatcacgg gatagagaaa
      721 acatgctgtg aatcttccgg agcaaaatgc tgcagaaaag aatgtttaaa attaatgaaa
      781 taccttttgg aacagttgaa aaaagagttt caagagctgg atgcattctg ttcctaccat
      841 gtgaaaactg ccatctttca catgtggacc caggacccgc aggacagtca gtgggacccc
      901 aggaacctca gctcctgctt cgataagttg ttagcattct ttcttgagtg cctcaggaca
      961 gagaaactgg atcattattt tattccaaag ttcaatctat tctctcaaga actaattgac
     1021 cgaaaaagta aagaatttct atcgaagaaa attgaatatg aaagaaataa tgggtttcca
     1081 atttttgaca agctttaccc atacgatgtt ccagattacg cttgagtcga c
//
