LOCUS       sequence-250876-       13016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of rat Neu cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13016)
  AUTHORS   Du Z, Podsypanina K, Huang S, McGrath A, Toneff MJ, Bogoslovskaia E,
            Zhang X, Moraes RC, Fluck M, Allred DC, Lewis MT, Varmus HE, Li Y
  TITLE     Introduction of oncogenes into mammary glands in vivo with an avian 
            retroviral vector initiates and promotes carcinogenesis in mouse 
            models.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17396-401. doi: 
            10.1073/pnas.0608607103. Epub 2006 Nov 7.
  PUBMED    17090666
REFERENCE   2  (bases 1 to 13016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0608607103"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2006-11-14- 14"; volume: "103"; issue: "46"; pages: 
            "17396-401"
COMMENT     Sequence Label: RCAS-Neu HA SIINFEKL
FEATURES             Location/Qualifiers
     source          1..13016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1288..1314
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1315..1341
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1348..1371
                     /codon_start=1
                     /product="antigenic CD8+ T cell epitope from chicken 
                     ovalbumin"
                     /label=OVA peptide
                     /translation="SIINFEKL"
     promoter        1516..1777
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2367..2389)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2378..2394
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2402..2408,2409..2426,2427..2432))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(2475..2493)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2561..2665
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2666..2734,2735..3526)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2884..2903)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3697..4285
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4186..4205
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4439..4456
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4471..4611
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4537..4556)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4697..4719
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4713..4904)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        5747..6008
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 atgatcatca tggagctggt ggcctggtgc cgctgggggt tcctcctcgc cctcctgccc
       61 cccggaatcg cgggcaccca agtgtgtcca agcttggtat gccagccgtg ccccatcaac
      121 tgcacccact cctgtgtgga tctggatgaa cgaggctgcc cagcagagca gagagccagc
      181 ccggtgacat tcatcattgc aactgtagag ggcgtcctgc tgttcctgat cttagtggtg
      241 gtcgttggaa tcctaatcaa acgaaggaga cagaagatcc ggaagtatac gatgcgtagg
      301 ctgctgcagg aaactgagtt agtggagccg ctgacgccca gcggagcaat gcccaaccag
      361 gctcagatgc ggatcctaaa agagacggag ctaaggaagg tgaaggtgct tggatcagga
      421 gcttttggca ctgtctacaa gggcatctgg atcccagatg gggagaatgt gaaaatcccc
      481 gtggctatca aggtgttgag agaaaacaca tctcctaaag ccaacaaaga aattctagat
      541 gaagcgtatg tgatggctgg tgtgggttct ccgtatgtgt cccgcctcct gggcatctgc
      601 ctgacatcca cagtacagct ggtgacacag cttatgccct acggctgcct tctggaccat
      661 gtccgagaac accgaggtcg cctaggctcc caggacctgc tcaactggtg tgttcagatt
      721 gccaagggga tgagctacct ggaggacgtg cggcttgtac acagggacct ggctgcccgg
      781 aatgtgctag tcaagagtcc caaccacgtc aagattacag atttcgggct ggctcggctg
      841 ctggacattg atgagacaga gtaccatgca gatgggggca aggtgcccat caaatggatg
      901 gcattggaat ctattctcag acgccggttc acccatcaga gtgatgtgtg gagctatgga
      961 gtgactgtgt gggagctgat gacttttggg gccaaacctt acgatggaat cccagcccgg
     1021 gagatccctg atttgctgga gaagggagaa cgcctacctc agcctccaat ctgcaccatt
     1081 gatgtctaca tgattatggt caaatgttgg atgattgact ctgaatgtcg cccgagattc
     1141 cgggagttgg tgtcagaatt ttcacgtatg gcgagggacc cccagcgttt tgtggtcatc
     1201 cagaacgagg acttgggccc atccagcccc atggctgcag agaaccctga gtacctaggc
     1261 ctggatgtac ctgtagtagg taccgtatac ccatacgacg tcccagacta cgcttaccca
     1321 tacgacgtcc cagactacgc tcttgagagt ataatcaact ttgaaaaact gtgacgcatc
     1381 gatgcgatgt acgggccaga tatacgcgta tctgagggga ctagggtgtg tttaggcgaa
     1441 aagcggggct tcggttgtac gcggttagga gtcccctcag gatatagtag tttcgctttt
     1501 gcatagggag ggggaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     1561 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     1621 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     1681 acgaaccact gaattccgca ttgcagagat attgtattta agtgcctagc tcgatacaat
     1741 aaacgccatt tgaccattca ccacattggt gtgcacctgg gttgatggcc ggaccgttga
     1801 ttccctgacg actacgagca cctgcatgaa gcagaaggct tcatttggtg accccgacgt
     1861 gatagttagg gaatagtggt cggccacaga cggcgtggcg atcctgtctc catccgtctc
     1921 gtctatcggg aggcgagttc gatgaccctg gtggaggggg ctgcggctta gggaggcaga
     1981 agctgagtac cgtcggaggg gatccacagg acgggtgtgg tcgccatgat cgcgtagtcg
     2041 atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt
     2101 gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca
     2161 tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc
     2221 ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca
     2281 ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg
     2341 cattaaagct ccaagcttgg ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     2401 acattatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagaattc
     2461 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     2521 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     2581 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     2641 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     2701 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     2761 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     2821 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     2881 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
     2941 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     3001 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     3061 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     3121 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     3181 aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt
     3241 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     3301 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     3361 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     3421 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     3481 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     3541 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     3601 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3661 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3721 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3781 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3841 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3901 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3961 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4021 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4081 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4141 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4201 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4261 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4321 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     4381 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     4441 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     4501 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     4561 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     4621 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     4681 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     4741 cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct
     4801 gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg
     4861 ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa
     4921 ggaggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata
     4981 cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg
     5041 gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat
     5101 acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata
     5161 atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc
     5221 attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg
     5281 cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc
     5341 aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc
     5401 cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc
     5461 gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta
     5521 gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca
     5581 acgcggggag gcagacaagg tatagggcgg cgatgcgatg tacgggccag atatacgcgt
     5641 atctgagggg actagggtgt gtttaggcga aaagcggggc ttcggttgta cgcggttagg
     5701 agtcccctca ggatatagta gtttcgcttt tgcataggga gggggaaatg tagtcttatg
     5761 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     5821 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     5881 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattccgc attgcagaga
     5941 tattgtattt aagtgcctag ctcgatacaa taaacgccat ttgaccattc accacattgg
     6001 tgtgcacctg ggttgatggc cggaccgttg attccctgac gactacgagc acctgcatga
     6061 agcagaaggc ttcatttggt gaccccgacg tgatagttag ggaatagtgg tcggccacag
     6121 acggcgtggc gatcctgtct ccatccgtct cgtctatcgg gaggcgagtt cgatgaccct
     6181 ggtggagggg gctgcggctt agggaggcag aagctgagta ccgtcggagg gagctccagg
     6241 gcccggagcg actgacccct gccgagaact cagagggtcg tcggaagacg gagagtgagc
     6301 ccgacgacca ccccaggcac gtctttggtc ggcctgcgga tcaagcatgg aagccgtcat
     6361 taaggtgatt tcgtccgcgt gtaaaaccta ttgcgggaaa atctctcctt ctaagaagga
     6421 aataggggcc atgttgtccc tgttacaaaa ggaagggttg cttatgtctc cctcagattt
     6481 atattctccg gggtcctggg atcccatcac tgcggcgctc tcccagcggg caatggtact
     6541 tggaaaatcg ggagagttaa aaacctgggg attggttttg ggggcattga aggcggctcg
     6601 agaggaacag gttacatctg agcaagcaaa gttttggttg ggattagggg gagggagggt
     6661 ctctccccca ggtccggagt gcatcgagaa accagctacg gagcggcgaa tcgacaaagg
     6721 ggaggaggtg ggagaaacaa ctgtgcagcg agatgcgaag atggcgccag aggaagcggc
     6781 cacacctaaa accgttggca catcctgcta tcattgcgga acagctgttg gctgcaattg
     6841 cgccaccgcc acagcctcgg cccctcctcc cccttatgtg gggagtggtt tgtatccttc
     6901 cctggcgggg gtgggagagc agcagggcca gggagataac acgtctcggg gggcggagca
     6961 gccaagggag gagccagggc acgcgggtca ggcccctggg ccggccctga ctgactgggc
     7021 aagggtaagg gaggagcttg cgagtactgg tccgcccgtg gtggccatgc ctgtagtgat
     7081 taagacagag ggacccgcct ggacccctct ggagccaaaa ttgatcacaa gactggctga
     7141 tacggtcagg accaagggct tacgatcccc gatcactatg gcagaagtgg aagcgctcat
     7201 gtcctccccg ttgctgccgc atgacgtcac gaatctaatg agagtgattt taggacctgc
     7261 cccatatgcc ttatggatgg acgcttgggg agtccaactc cagacggtta tagcggcagc
     7321 cactcgcgac ccccgacacc cagcgaacgg tcaagggcgg ggggaacgga ctaacttgga
     7381 tcgattaaag ggcttagctg atgggatggt gggcaaccca cagggtcagg ccgcattatt
     7441 aagaccgggg gaattggttg ctattacggc gtcggctctc caggcgttta gagaagttgc
     7501 ccggctggcg gaacctgcag gtccatgggc ggacatcacg cagggaccat ctgagtcctt
     7561 tgttgatttt gccaatcggc ttataaaggc ggttgagggg tcagatctcc cgccttccgc
     7621 gcgggctccg gtgatcattg actgctttag gcagaagtca cagccagata ttcagcagct
     7681 tatacgggca gcaccctcca cgctgaccac cccaggagag ataatcaaat atgtgctaga
     7741 caggcagaag attgcccctc ttacggatca aggcatagcc gcggccatgt cgtctgctat
     7801 ccagccctta gttatggcag tagtcaatag agagagggat ggacaaactg ggtcgggtgg
     7861 tcgtgcccga gggctctgct acacttgtgg atccccggga cattatcagg cacagtgccc
     7921 gaaaaaacga aagtcaggaa acagccgtga gcgatgtcag ctgtgtgacg ggatgggaca
     7981 caacgctaaa cagtgtagga agcgggatgg caaccagggc caacgcccag gaagaggtct
     8041 ctcttcgggg ccgtggcccg gccctgagca gcctgccgtc tcgttagcga tgacaatgga
     8101 acataaagat cgccccttgg ttagggtcat tctgactaac actgggagtc atccagtcaa
     8161 acaacgttcg gtgtatatca ccgcgctgtt ggactccgga gcggacatca ctattatttc
     8221 ggaggaggat tggcctactg attggccggt ggtggacacc gcgaacccac agatccatgg
     8281 cataggaggg ggaattccca tgcgaaaatc ccgggatatg atagaggtgg gggttattaa
     8341 ccgagacggg tcgttggagc gacccctgct cctcttcccc gcagtcgcta tggttagagg
     8401 gagtatccta ggaagagatt gtctgcaggg cctagggctc cgcttgacaa atttataggg
     8461 agggccactg ttcttactgt tgcgctacat ctggctattc cgctcaaatg gaagtcagac
     8521 cgcacgcctg tgtggattga ccagtggccc cttcctgaag gtaaacttgt agcgctaacg
     8581 caattagtgg aaaaagaatt acagttagga catatagagc cctcacttag ttgttggaac
     8641 acacctgttt ttgtgatccg gaaggcttcc gggtcttatc gcttattgca tgatttgcgc
     8701 gctgttaacg ccaagcttgt tccttttggg gccgtccaac agggggcgcc agttctctcc
     8761 gcgctcccgc gtggctggcc cctgatggtc ctagacctca aggattgctt cttttctatc
     8821 cctcttgcgg aacaagatcg cgaagctttt gcatttacgc tcccctctgt gaataaccag
     8881 gcccccgctc gaagattcca atggaaggtc ttgccccaag ggatgacctg ttctcccact
     8941 atctgtcagt tggtagtggg tcaggtgctc gagcccttgc gactcaagca cccagctctg
     9001 cgcatgttgc attatatgga cgatcttttg ctagccgcct caagtcatga tgggttggaa
     9061 gcggcaggga aggaggttat cggtacattg gaaagagccg ggttcactat ttcgccggat
     9121 aagatccaga gggagcccgg agtacaatat cttgggtaca agttaggcag tacgtatgta
     9181 gcacccgtag gcttggtagc agaacccagg atagccacct tgtgggatgt tcaaaagctg
     9241 gtggggtcac ttcagtggct tcgcccagcg ttagggatcc cgccacgact gatgggtccc
     9301 ttttatgagc agttacgagg gtcagatcct aacgaggcga gggaatggaa tctagacatg
     9361 aaaatggcct ggagagagat cgtacagctt agcactactg ctgccttgga acgatgggac
     9421 cctgcccagc ctctggaagg agcggtcgct agatgtgaac agggggcaat aggggtcctg
     9481 ggacagggac tgtccacaca cccaaggcca tgtttgtggt tattctccac ccaacccacc
     9541 aaggcgttta ctgcttggtt agaagtgctc acccttttga ttactaagct acgcgcttcg
     9601 gcagtgcgaa cctttggcaa ggaggttgat atcctcctgt tgcctgcatg cttccgggag
     9661 gaccttccgc tcccggaggg gatcctgtta gcacttaggg ggtttgcagg aaaaatcagg
     9721 agtagtgaca cgccatctat ttttgacatt gcgcgtccac tgcatgtttc tctgaaagtg
     9781 agggttaccg accaccctgt gccgggaccc actgtcttta ccgacgcctc ctcaagcacc
     9841 cataaagggg tggtagtctg gagggagggc ccaaggtggg agataaaaga aatagttgat
     9901 ttgggggcaa gtgtacaaca actggaggca cgcgctgtgg ccatggcact tctgctgtgg
     9961 ccgacaacgc ccactaatgt agtgactgac tctgcgtttg ttgcgaaaat gttactcaag
    10021 atgggacagg agggagtccc gtctacagcg gcagctttta ttttagagga tgcgttaagc
    10081 caaaggtcag ccatggccgc cgttctccac gtgcggagtc attctgaagt gccagggttt
    10141 ttcacagaag gaaatgacgt ggcagatagc caagccacct ttcaagcgta tcccttgaga
    10201 gaggctaaag atcttcatac cgctctccat attggacccc gcgcgctatc caaagcgtgt
    10261 aatatatcta tgcagcaggc tagggaggtt gttcagacct gcccgcattg taattcagcc
    10321 cctgcgttgg aggccggggt aaaccctagg ggtttgggac ccctacagat atggcagaca
    10381 gactttacgc ttgagcctag aatggctccc cgttcctggc tcgctgttac tgtggacacc
    10441 gcctcatcag cgatagtcgt aactcagcat ggccgtgtta catcggttgc tgcacaacat
    10501 cattgggcca cggctatcgc cgttttggga agaccaaagg ccataaaaac agataacggg
    10561 tcctgcttca cgtctaaatc cacgcgggag tggctcgcga gatgggggat agcacacacc
    10621 accgggattc cgggaaattc ccagggtcaa gctatggtag agcgggccaa ccggctcctg
    10681 aaagataaga tccgtgtgct tgcggaaggg gacggcttta tgaaaagaat ccccgccagc
    10741 aaacaggggg aactactagc caaagcaatg tatgccctca atcactttga gcgtggtgaa
    10801 aacacaaaaa caccggtaca aaaacactgg agacctaccg ttcttacaga aggacccccg
    10861 gttaaaatac gaatagagac aggggagtgg gaaaaaggat ggaacgtgct ggtctgggga
    10921 cgaggttatg ccgctgtgaa aaacagggac actgataagg ttatttgggt accctctcgg
    10981 aaagttaaac cggatgtcac ccaaaaggat gaggtgacta agaaagatga ggcgagccct
    11041 ctttttgcag gcatttctga ctggataccc tgggaagacg agcaagaagg actccaagga
    11101 gaaaccgcta gcaacaagca agaaagaccc ggagaagaca cccttgctgc caacgagagt
    11161 taattatatt ctcattattg gtgtcctggt cttgtgtgag gttacggggg taagagctga
    11221 tgtccactta ctcgagcagc cagggaacct ttggattaca tgggccaacc gtacaggcca
    11281 aacggatttt tgcctctcta cacagtcagc cacctcccct tttcaaacat gtttgatagg
    11341 tatcccgtcc cctatttccg agggtgattt taagggatat gtttctgata caaattgcac
    11401 caccttggga actgatcggt tagtctcgtc agccgacttt actggcggac ctgacaacag
    11461 taccaccctc acttatcgga aggtctcatg cttgttgtta aagctgaatg tctctatgtg
    11521 ggatgagcca cctgaactac agctgttagg ttcccagtct ctccctaaca ttactaatat
    11581 tgctcagatt tccggtataa ccgggggatg cgtaggcttc agaccacaag gggttccttg
    11641 gtatctaggt tggtctagac aggaggccac gcggtttctc cttagacacc cctctttctc
    11701 taaatccacg gaaccgttta cggtggtgac agcggatagg cacaatcttt ttatggggag
    11761 tgagtactgc ggtgcatatg gctacagatt ttggaacatg tataactgct cacaggtggg
    11821 gcggcagtac cgctgtggta atgcgcgcag cccccgcccg ggtcttcctg aaatccagtg
    11881 tacaaggaga ggaggcaaat gggttaatca atcacaggaa attaatgagt cggagccgtt
    11941 cagctttacg gtgaactgta cagctagtag tttgggtaat gccagtgggt gttgcggaaa
    12001 agcaggcacg attctcccgg gaaagtgggt cgacagcaca caaggtagtt tcaccaaacc
    12061 aaaagcgcta ccacccgcaa ttttcctcat ttgtggggat cgcgcatggc aaggaattcc
    12121 cagtcgtccg gtagggggcc cctgctattt aggcaagctt accatgttag cacctaagca
    12181 tacagatatt ctcaaggtgc ttgtcaattc atcgcggaca ggtataagac gtaaacgaag
    12241 cacctcacac ctggatgata catgctcaga tgaagtgcag ctttggggtc ctacagcaag
    12301 aatctttgca tctatcctag ccccgggggt agcagctgcg caagccttaa gagaaattga
    12361 gagactagcc tgttggtccg ttaaacaggc taacttgaca acatcactcc tcggggactt
    12421 attggatgat gtcacgagta ttcgacacgc ggtcctgcag aaccgagcgg ctattgactt
    12481 cttgctccta gctcacggcc atggctgtga ggacgttgcc ggaatgtgct gtttcaattt
    12541 gagtgatcac agtgagtcta tacagaagaa gttccagcta atgaaggaac atgtcaataa
    12601 gatcggcgtg gacagcgacc caattggaag ttggctgcga ggactattcg ggggaatagg
    12661 agaatgggcc gttcatttgc tgaaaggact gcttttgggg cttgtagtta ttttgttgct
    12721 agtagtgtgc ctgccttgcc ttttgcaaat cgtatgcggt aacatcagaa agatgattaa
    12781 taactccatc agctaccaca cggaatataa gaagctgcaa aaggcctgtg ggcagcctga
    12841 aagcagaata gtataaggca gtacatgggt ggtggtatag cgcttgcgag tccatcgagg
    12901 aaggcaggaa agacagctat tggtaattgt gaaatacgct tttgtctgtg tgctgcagga
    12961 gctgagctga ctctgctggt ggcctcgcgt accactgtgg catcggcggc cgcgca
//
