LOCUS       sequence-250880-        1110 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A CRISPR activation device with the necessary genes (dcas9 
            controlled by Ptet, pspF-Delta-HTH::lambda-N22plus controlled by 
            promoter J23106), and the reporter part (PpspA-LEA5B5 with 
            sfgfp::ASV)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Liu Y, Wan X, Wang B
  TITLE     Engineered CRISPRa enables programmable eukaryote-like gene 
            activation in bacteria.
  JOURNAL   Nat Commun. 2019 Aug 26;10(1):3693. doi: 10.1038/s41467-019-11479-0.
  PUBMED    31451697
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-26"; volume: "10(1):3693. doi"; pages: " 
            10.1038/s41467-019-11479-0"
COMMENT     Sequence Label: pLY57
FEATURES             Location/Qualifiers
     source          1..1110
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        191..240
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             273..284
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             291..1004
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 tactagaagt tatatgttgg taactaccgg ggatgaatat ataagtgcgg ttagtgtaat
       61 tcgctaactc atcctggcat gttgctgttg attcttcaat cagatcttta taaatcaaaa
      121 agataaaaaa ttggcacgca aattgtatta acagttcagc aggacaatcc tgaacgcaaa
      181 tactagagag ctgtcaccgg atgtgctttc cggtctgatg agtccgtgag gacgaaacag
      241 cctctacaaa taattttgtt taactagaga ttaaagagga gaaataccat atgcgtaaag
      301 gcgaagagct gttcactggt gtcgtcccta ttctggtgga actggatggt gatgtcaacg
      361 gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt aaactgacgc
      421 tgaagttcat ctgtactact ggtaaactgc cggtaccttg gccgactctg gtaacgacgc
      481 tgacttatgg tgttcagtgc tttgctcgtt atccggacca tatgaagcag catgacttct
      541 tcaagtccgc catgccggaa ggctatgtgc aggaacgcac gatttccttt aaggatgacg
      601 gcacgtacaa aacgcgtgcg gaagtgaaat ttgaaggcga taccctggta aaccgcattg
      661 agctgaaagg cattgacttt aaagaagacg gcaatatcct gggccataag ctggaataca
      721 attttaacag ccacaatgtt tacatcaccg ccgataaaca aaaaaatggc attaaagcga
      781 attttaaaat tcgccacaac gtggaggatg gcagcgtgca gctggctgat cactaccagc
      841 aaaacactcc aatcggtgat ggtcctgttc tgctgccaga caatcactat ctgagcacgc
      901 aaagcgttct gtctaaagat ccgaacgaga aacgcgatca tatggttctg ctggagttcg
      961 taaccgcagc gggcatcacg catggtatgg atgaactgta caaaaggcct gctgcaaacg
     1021 acgaaaacta cgctgcatca gtttaataat actagagcca attattgaag gcctccctaa
     1081 cggggggcct ttttttgttt ctggtctccc
//
