LOCUS       sequence-250889-        6048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encodes A. thaliana codon optimized Integrase Bxb1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Gomide MS, Sales TT, Barros LRC, Limia CG, de Oliveira MA, 
            Florentino LH, Barros LMG, Robledo ML, Jose GPC, Almeida MSM, Lima 
            RN, Rehen SK, Lacorte C, Melo EO, Murad AM, Bonamino MH, Coelho CM, 
            Rech E
  TITLE     Genetic switches designed for eukaryotic cells and controlled by 
            serine integrases.
  JOURNAL   Commun Biol. 2020 May 22;3(1):255. doi: 10.1038/s42003-020-0971-8.
  PUBMED    32444777
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-05-22"; volume: "3(1):255. doi"; pages: " 
            10.1038/s42003-020-0971-8"
COMMENT     Sequence Label: INCTbiosyn-pAct-AtINTBxb1
FEATURES             Location/Qualifiers
     source          1..6048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    93..114
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(129..134,135..152,153..159)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    167..183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     172..194
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     191..207
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     191..207
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        228..246
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      3201..3454
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3463..3482)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3464..3482)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3489..3506)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3489..3505)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3498..3520)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(3647..4102)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3784..3805)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3996..4015
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4129..4233
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4234..4302,4303..5094)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4452..4471)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5265..5853
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5754..5773
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6007..6024
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
       61 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
      121 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
      181 caatttcaca caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac
      241 taaagggaac aaaagctgaa aatttagaac gaacttaatt atgatctcaa atacattgat
      301 acatatctca tctagatcta ggttatcatt atgtaagaaa gttttgacga atatggcacg
      361 acaaaatggc tagactcgat gtaattggta tctcaactca acattatact tataccaaac
      421 attagttaga caaaatttaa acaactattt tttatgtatg caagagtcag catatgtata
      481 attgattcag aatcgttttg acgagttcgg atgtagtagt agccattatt taatgtacat
      541 actaatcgtg aatagtgaat atgatgaaac attgtatctt attgtataaa tatccataaa
      601 cacatcatga aagacacttt ctttcacggt ctgaattaat tatgatacaa ttctaataga
      661 aaacgaatta aattacgttg aattgtatga aatctaattg aacaagccaa ccacgacgac
      721 gactaacgtt gcctggattg actcggttta agttaaccac taaaaaaacg gagctgtcat
      781 gtaacacgcg gatcgagcag gtcacagtca tgaagccatc aaagcaaaag aactaatcca
      841 agggctgaga tgattaatta gtttaaaaat tagttaacac gagggaaaag gctgtctgac
      901 agccaggtca cgttatcttt acctgtggtc gaaatgattc gtgtctgtcg attttaatta
      961 tttttttgaa aggccgaaaa taaagttgta agagataaac ccgcctatat aaattcatat
     1021 attttcctct ccgctttgaa ttgtctcgtt gtcctcctca ctttcatcag ccgttttgaa
     1081 tctccggcga cttgacagag aagaacaagg aagaagacta agagagaaag taagagataa
     1141 tccaggagat tcattctccg ttttgaatct tcctcaatct catcttcttc cgctctttct
     1201 ttccaaggta ataggaactt tctggatcta ctttatttgc tggatctcga tcttgttttc
     1261 tcaatttcct tgagatctgg aattcgttta atttggatct gtgaacctcc actaaatctt
     1321 ttggttttac tagaatcgat ctaagttgac cgatcagtta gctcgattat agctaccaga
     1381 atttggcttg accttgatgg agagatccat gttcatgtta cctgggaaat gatttgtata
     1441 tgtgaattga aatctgaact gttgaagtta gattgaatct gaacactgtc aatgttagat
     1501 tgaatctgaa cactgtttaa ggttagatga agtttgtgta tagattcttc gaaactttag
     1561 gatttgtagt gtcgtacgtt gaacagaaag ctatttctga ttcaatcagg gtttatttga
     1621 ctgtattgaa ctctttttgt gtgtttgcag ctcataaaaa caagcttcct aggatgaggg
     1681 ccttagtcgt gatccgacta tccagggtca ctgatgccac cacatcacca gaaagacaac
     1741 tagagtcatg ccagcaattg tgcgcacaaa gaggatggga tgttgtaggc gttgcggagg
     1801 acctagatgt atcaggtgcc gtggacccct ttgatagaaa acgaagacca aacctagcgc
     1861 gttggctcgc ttttgaagag caaccgtttg atgtgatcgt ggcatacagg gtggatagac
     1921 taactaggag tatacgacac ctacagcagt tagtacattg ggctgaggat cataagaagc
     1981 tagtggtctc tgcaactgag gcccatttcg acacgaccac cccttttgcc gcggttgtca
     2041 ttgcgttaat gggcaccgta gcacaaatgg aactcgaggc aatcaaggaa agaaaccgaa
     2101 gcgcagcaca ctttaacatt cgagccggga agtaccgagg ctctctgccc ccttggggct
     2161 atctcccgac gagggtggat ggtgaatgga gattagtacc agaccccgtc cagagggaaa
     2221 ggattctaga agtgtaccac cgagtggtgg ataatcacga accgctccac ctcgtggcgc
     2281 acgacttaaa ccgtcgtggg gtgctctctc caaaggacta ttttgcgcag cttcagggtc
     2341 gtgaacctca ggggcgagag tggtccgcta ctgctttgaa acgtagtatg attagcgagg
     2401 caatgttagg atatgctacg ctaaacggca aaaccgtccg agatgatgac ggcgcgccgc
     2461 tagtacgagc tgaacctata ctgacaagag aacagcttga ggctttacgt gcggagttag
     2521 ttaagactag cagggcaaaa ccagcagtta gtacgccttc actgttactc agggttctgt
     2581 tttgcgcagt ctgtggtgag cccgcgtaca aattcgctgg tggggggcgt aagcacccgc
     2641 gttaccgttg tcgtagtatg ggcttcccaa agcattgcgg gaatggaacc gtggcaatgg
     2701 cggagtggga cgctttctgc gaggagcaag tattggatct cctcggggat gctgaacgac
     2761 tggagaaagt gtgggtagct gggtctgatt ctgctgtcga gctagcggaa gtaaatgctg
     2821 aactagtgga cttaacgtcc ctcattgggt ctccggcata tagagctggc agccctcaaa
     2881 gggaagccct tgatgcgcgt atcgccgccc tcgcagcgag acaggaagaa ttggaggggc
     2941 tggaggctag gccatcaggc tgggagtggc gagaaacggg gcagcgattt ggtgattggt
     3001 ggagagagca ggatacggcc gctaaaaata cgtggcttag gagtatgaac gtgcgtctga
     3061 cgtttgatgt tcgtggtggt ctcacacgaa ctattgactt tggtgacttg caagaatacg
     3121 agcagcactt acgattggga agtgttgttg aacgtctaca tacgggcatg agttagggta
     3181 ccgcggccgc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga
     3241 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
     3301 taatatttaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
     3361 cgcaattata catttaatac gcgatagaaa acaaaatatc gcgcgcaaac ttggataaat
     3421 tatcgcgcgc ggtgtcatct atgaggacta gatcgcaatt cgccctatag tgagtcgtat
     3481 tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     3541 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     3601 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcaaat tgtaagcgtt
     3661 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     3721 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     3781 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     3841 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     3901 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     3961 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     4021 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     4081 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4141 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4201 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4261 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4321 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     4381 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     4441 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     4501 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     4561 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4621 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4681 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4741 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     4801 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     4861 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     4921 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     4981 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5041 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5101 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5161 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5221 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5281 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5341 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5401 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5461 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5521 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5581 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5641 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5701 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5761 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5821 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5881 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     5941 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     6001 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgc
//
