LOCUS       sequence-250931-        1255 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Engineered sgRNA-G6-2MS2 (2 MS2 aptamers) generator circuit 
            including promoter Pbad.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Liu Y, Wan X, Wang B
  TITLE     Engineered CRISPRa enables programmable eukaryote-like gene 
            activation in bacteria.
  JOURNAL   Nat Commun. 2019 Aug 26;10(1):3693. doi: 10.1038/s41467-019-11479-0.
  PUBMED    31451697
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-26"; volume: "10(1):3693. doi"; pages: " 
            10.1038/s41467-019-11479-0"
COMMENT     Sequence Label: pLY124
FEATURES             Location/Qualifiers
     source          1..1255
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(66..944)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        971..1255
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1178..1197
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 ggaccaaaac gaaaaaagac gctcgaaagc gtctcttttc tggaatttgg taccgagctc
       61 tagtattatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac
      121 tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca
      181 aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc
      241 gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg
      301 aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca
      361 aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc
      421 atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc
      481 agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc
      541 ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg
      601 gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc
      661 cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc
      721 gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc
      781 ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata
      841 aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta
      901 aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg
      961 ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt
     1021 tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa
     1081 agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa
     1141 gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata
     1201 agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc tccat
//
