LOCUS       sequence-250947-        1094 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dcas9 generator and reporter circuit (PpspA-2G6 with sfgfp::ASV).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Liu Y, Wan X, Wang B
  TITLE     Engineered CRISPRa enables programmable eukaryote-like gene 
            activation in bacteria.
  JOURNAL   Nat Commun. 2019 Aug 26;10(1):3693. doi: 10.1038/s41467-019-11479-0.
  PUBMED    31451697
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-26"; volume: "10(1):3693. doi"; pages: " 
            10.1038/s41467-019-11479-0"
COMMENT     Sequence Label: pLY152
FEATURES             Location/Qualifiers
     source          1..1094
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        175..224
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             257..268
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             275..988
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 tactagaaaa gctgttgtgg caaaaaacgg aaagctgttg tggcaaaaaa cggcatcctg
       61 gcatgttgct gttgattctt caatcagatc tttataaatc aaaaagataa aaaattggca
      121 cgcaaattgt attaacagtt cagcaggaca atcctgaacg caaatactag agagctgtca
      181 ccggatgtgc tttccggtct gatgagtccg tgaggacgaa acagcctcta caaataattt
      241 tgtttaacta gagattaaag aggagaaata ccatatgcgt aaaggcgaag agctgttcac
      301 tggtgtcgtc cctattctgg tggaactgga tggtgatgtc aacggtcata agttttccgt
      361 gcgtggcgag ggtgaaggtg acgcaactaa tggtaaactg acgctgaagt tcatctgtac
      421 tactggtaaa ctgccggtac cttggccgac tctggtaacg acgctgactt atggtgttca
      481 gtgctttgct cgttatccgg accatatgaa gcagcatgac ttcttcaagt ccgccatgcc
      541 ggaaggctat gtgcaggaac gcacgatttc ctttaaggat gacggcacgt acaaaacgcg
      601 tgcggaagtg aaatttgaag gcgataccct ggtaaaccgc attgagctga aaggcattga
      661 ctttaaagaa gacggcaata tcctgggcca taagctggaa tacaatttta acagccacaa
      721 tgtttacatc accgccgata aacaaaaaaa tggcattaaa gcgaatttta aaattcgcca
      781 caacgtggag gatggcagcg tgcagctggc tgatcactac cagcaaaaca ctccaatcgg
      841 tgatggtcct gttctgctgc cagacaatca ctatctgagc acgcaaagcg ttctgtctaa
      901 agatccgaac gagaaacgcg atcatatggt tctgctggag ttcgtaaccg cagcgggcat
      961 cacgcatggt atggatgaac tgtacaaaag gcctgctgca aacgacgaaa actacgctgc
     1021 atcagtttaa taatactaga gccaattatt gaaggcctcc ctaacggggg gccttttttt
     1081 gtttctggtc tccc
//
