LOCUS       sequence-250949-        1087 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  reporter circuit (PpspA-2G6 with sfgfp::ASV).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Liu Y, Wan X, Wang B
  TITLE     Engineered CRISPRa enables programmable eukaryote-like gene 
            activation in bacteria.
  JOURNAL   Nat Commun. 2019 Aug 26;10(1):3693. doi: 10.1038/s41467-019-11479-0.
  PUBMED    31451697
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-26"; volume: "10(1):3693. doi"; pages: " 
            10.1038/s41467-019-11479-0"
COMMENT     Sequence Label: pLY153
FEATURES             Location/Qualifiers
     source          1..1087
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        168..217
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             250..261
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             268..981
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 aaagctgttg tggcaaaaaa cggaaagctg ttgtggcaaa aaacggcatc ctggcatgtt
       61 gctgttgatt cttcaatcag atctttataa atcaaaaaga taaaaaattg gcacgcaaat
      121 tgtattaaca gttcagcagg acaatcctga acgcaaatac tagagagctg tcaccggatg
      181 tgctttccgg tctgatgagt ccgtgaggac gaaacagcct ctacaaataa ttttgtttaa
      241 ctagagatta aagaggagaa ataccatatg cgtaaaggcg aagagctgtt cactggtgtc
      301 gtccctattc tggtggaact ggatggtgat gtcaacggtc ataagttttc cgtgcgtggc
      361 gagggtgaag gtgacgcaac taatggtaaa ctgacgctga agttcatctg tactactggt
      421 aaactgccgg taccttggcc gactctggta acgacgctga cttatggtgt tcagtgcttt
      481 gctcgttatc cggaccatat gaagcagcat gacttcttca agtccgccat gccggaaggc
      541 tatgtgcagg aacgcacgat ttcctttaag gatgacggca cgtacaaaac gcgtgcggaa
      601 gtgaaatttg aaggcgatac cctggtaaac cgcattgagc tgaaaggcat tgactttaaa
      661 gaagacggca atatcctggg ccataagctg gaatacaatt ttaacagcca caatgtttac
      721 atcaccgccg ataaacaaaa aaatggcatt aaagcgaatt ttaaaattcg ccacaacgtg
      781 gaggatggca gcgtgcagct ggctgatcac taccagcaaa acactccaat cggtgatggt
      841 cctgttctgc tgccagacaa tcactatctg agcacgcaaa gcgttctgtc taaagatccg
      901 aacgagaaac gcgatcatat ggttctgctg gagttcgtaa ccgcagcggg catcacgcat
      961 ggtatggatg aactgtacaa aaggcctgct gcaaacgacg aaaactacgc tgcatcagtt
     1021 taataatact agagccaatt attgaaggcc tccctaacgg ggggcctttt tttgtttctg
     1081 gtctccc
//
