LOCUS       sequence-25097-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Noguchi C, Garabedian MV, Malik M, Noguchi E
  TITLE     A vector system for genomic FLAG epitope-tagging in 
            Schizosaccharomyces pombe.
  JOURNAL   Biotechnol J. 2008 Oct;3(9-10):1280-5. doi: 10.1002/biot.200800140.
  PUBMED    18729046
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J."; date: "2008-10"; volume: "3(9-10):1280-5. doi"; pages: " 
            10.1002/biot.200800140"
COMMENT     Sequence Label: pFA6a-kanMX6-P3nmt1-3FLAG
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(60..247)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     168..192
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(271..342)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 cgcgagctcg tcgctgcggt cgacggatct atattaccct gttatcccta gcggatctgc
       61 cggtagaggt gtggtcaata agagcgacct catactatac ctgagaaagc aacctgacct
      121 acaggaaaga gttactcaag aataagaatt ttcgttttaa aacctaagag tcactttaaa
      181 atttgtatac acttattttt tttataactt atttaataat aaaaatcata aatcataaga
      241 aattcgctta tttagaagtg gcgcgcccta tttatcatca tcgtccttat agtccttatc
      301 gtcgtcatct ttgtaatcct tgtcatcgtc gtccttgtag tccatattaa ttaaagacat
      361 gatttaacaa agcgactata agtcagaaag tgagaatgag attgaaataa ttaattcaac
      421 ttattcaatt gatgatatgc caggattcct cttcctttat atatcttcaa tgtttcgatt
      481 aacaggtcca ttatccggtt tttagtttcg ctgcacattg ccgaatgaca atcggattcc
      541 tttaagcttt gcttttgccc tgttttctct ccccagtaat cgttgggtga ctgaaccatc
      601 cagtagccga gagttacgtt tttcctgatt taataaatat ccatatctat ttgtctcgtg
      661 ggggtttaca gcaactancc gaaatnacta ttttggcatc atccaaccat aaagagacaa
      721 caataattct agaaaacata taacggggnc agttntctat ttccgccccn aaacgcnaag
      781 nccnctttac naggtttg
//
