LOCUS       sequence-25098-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Noguchi C, Garabedian MV, Malik M, Noguchi E
  TITLE     A vector system for genomic FLAG epitope-tagging in 
            Schizosaccharomyces pombe.
  JOURNAL   Biotechnol J. 2008 Oct;3(9-10):1280-5. doi: 10.1002/biot.200800140.
  PUBMED    18729046
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J."; date: "2008-10"; volume: "3(9-10):1280-5. doi"; pages: " 
            10.1002/biot.200800140"
COMMENT     Sequence Label: pFA6a-kanMX6-P41nmt1-3FLAG
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(61..248)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     169..193
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(272..343)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 gcgagctcgt cgctgcaggg tcgacggatc tatattaccc tgttatccct agcggatctg
       61 ccggtagagg tgtggtcaat aagagcgacc tcatactata cctgagaaag caacctgacc
      121 tacaggaaag agttactcaa gaataagaat tttcgtttta aaacctaaga gtcactttaa
      181 aatttgtata cacttatttt ttttataact tatttaataa taaaaatcat aaatcataag
      241 aaattcgctt atttagaagt ggcgcgccct atttatcatc atcgtcctta tagtccttat
      301 cgtcgtcatc tttgtaatcc ttgtcatcgt cgtccttgta gtccatatta attaaagaca
      361 tgatttaaca aagcgactat aagtcagaaa gtgagaatga gattgaaata attaattcaa
      421 cttattcaat tgatgatatg ccaggattcc tcttccttta tcttcaatgt ttcgattaac
      481 aggtccatta tccggttttt agtttcgctg cacattgccg aatgacaatc ggattccttt
      541 aagctttgct tttgccctgt tttctctccc cagtaatcgt tgggtgactg aaccatccag
      601 tagccgagag ttacgttttt cctgatttaa taaatatcca tatctatttg tctcgtggtg
      661 ttttacagca actaaccgaa attactattt tgcatcatcc aaccataaag agacaacaat
      721 aattctagaa aacatataac aagtgtttnt tctntttccn ncccancgca agncncgctt
      781 tccntgnttn ggaactgnnc cccaccatcn t
//
