LOCUS       sequence-25099-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Noguchi C, Garabedian MV, Malik M, Noguchi E
  TITLE     A vector system for genomic FLAG epitope-tagging in 
            Schizosaccharomyces pombe.
  JOURNAL   Biotechnol J. 2008 Oct;3(9-10):1280-5. doi: 10.1002/biot.200800140.
  PUBMED    18729046
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J."; date: "2008-10"; volume: "3(9-10):1280-5. doi"; pages: " 
            10.1002/biot.200800140"
COMMENT     Sequence Label: pFA6a-kanMX6-P81nmt1-3FLAG
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(57..244)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     165..189
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(268..339)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 cgactcgtcg cngcggtcga cggatctata ttaccctgtt atccctagcg gatctgccgg
       61 tagaggtgtg gtcaataaga gcgacctcat actatacctg agaaagcaac ctgacctaca
      121 ggaaagagtt actcaagaat aagaattttc gttttaaaac ctaagagtca ctttaaaatt
      181 tgtatacact tatttttttt ataacttatt taataataaa aatcataaat cataagaaat
      241 tcgcttattt agaagtggcg cgccctattt atcatcatcg tccttatagt ccttatcgtc
      301 gtcatctttg taatccttgt catcgtcgtc cttgtagtcc atattaatta aagacatgat
      361 ttaacaaagc gactataagt cagaaagtga gaatgagatt gaaataatta attcaactta
      421 ttcaattgat gatatgccag gattcctctt ccatcttcaa tgtttcgatt aacaggtcca
      481 ttatccggtt tttagtttcg ctgcacattg ccgaatgaca atcggattcc tttaagcttt
      541 gcttttgccc tgttttctct ccccagtaat cgttgggtga ctgaaccatc cagtagccga
      601 gagttacgtt tttcctgatt taataaatat ccatatctat ttgtctcgtg gtgttttaca
      661 gcaactaacc gaaattacta ttttgcatca tccaaccata aagagacnac aataattcta
      721 gaaaacatat aacaggtgnt tttttctatt tacgtctcat acgcnaagtc actttaccat
      781 ggtttgggaa ctggnacccc accatctttt tngattg
//
