LOCUS       sequence-250995-        6611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of the genetically encoded dopamine sensor 
            dLight1.3b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   Patriarchi T, Cho JR, Merten K, Howe MW, Marley A, Xiong WH, Folk 
            RW, Broussard GJ, Liang R, Jang MJ, Zhong H, Dombeck D, von Zastrow 
            M, Nimmerjahn A, Gradinaru V, Williams JT, Tian L
  TITLE     Ultrafast neuronal imaging of dopamine dynamics with designed 
            genetically encoded sensors.
  JOURNAL   Science. 2018 Jun 29;360(6396). pii: science.aat4422. doi: 
            10.1126/science.aat4422. Epub 2018 May 31.
  PUBMED    29853555
REFERENCE   2  (bases 1 to 6611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2018-06-29"; volume: "360(6396). pii: science.aat4422. doi"; 
            pages: " 10.1126/science.aat4422. Epub 2018 May 31"
COMMENT     Sequence Label: pAAV-CAG-dLight1.3b
FEATURES             Location/Qualifiers
     source          1..6611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   162..291
                     /label=AAV2 ITR
     primer_bind     1158..1180
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1242..1261
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     2272..2293
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2399..2420)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2660..2679)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    3365..3953
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3418..3438)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3836..3847)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3996..4117
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4007..4026)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4061..4080
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(4496..5084)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4576..4595)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5255..6046,6047..6115))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5878..5897
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6116..6220)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(6246..6611,1..90))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6383..6404)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     join(6595..6611,1..3)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
       61 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt
      121 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctgcgcgct cgctcgctca
      181 ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga
      241 gcgagcgagc gcgcagagag ggagtggcca actccatcac taggggttcc ttgtagttaa
      301 tgattaaccc gccatgctac ttatctacgt agccatgctc taggaagagt accattgacg
      361 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      421 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      481 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      541 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      601 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
      661 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
      721 gggcgcgcgc crggsggggs gggggggggs gggggggggg gsggggsgrg gsggrgrggk
      781 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
      841 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
      901 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
      961 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
     1021 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
     1081 ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct gccttcgggg
     1141 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc
     1201 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt
     1261 gctgtctcat cattttggca aagaattgga tccaccggtg cgcgccatga agacgatcat
     1321 cgccctgagc tacatcttct gcctggtgtt cgccgactac aaggacgatg atgacgccat
     1381 gaggactctg aacacctctg ccatggacgg gactgggctg gtggtggaga gggacttctc
     1441 tgttcgtatc ctcactgcct gtttcctgtc gctgctcatc ctgtccacgc tcctggggaa
     1501 cacgctggtc tgtgctgccg ttatcaggtt ccgacacctg cggtccaagg tgaccaactt
     1561 ctttgtcatc tccttggctg tgtcagatct cttggtggcc gtcctggtca tgccctggaa
     1621 ggcagtggct gagattgctg gcttctggcc ctttgggtcc ttctgtaaca tctgggtggc
     1681 ctttgacatc atgtgctcca ctgcatccat cctcaacctc tgtgtgatca gcgtggacag
     1741 gtattgggct atctccagcc ctgcccggta tgagagaaag atgaccccca aggcagcctt
     1801 catcctgatc agtgtggcat ggaccttgtc tgtactcatc tccttcatcc cagtgcagct
     1861 cagctggcac aaggcaaaac ccacaagccc ctctgatgga aatgccactt ccctggctga
     1921 gaccatagac aactgtgact ccagcctcag caggacatat gccatctcat cctctgtaat
     1981 cagcttttac atccctgtgg ccatcatgat tgtcacctac accaggatct acaggattgc
     2041 tcagaaacag ctgagctcac tcattaacgt ctatatcaag gccgacaagc agaagaacgg
     2101 catcaaggcg aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccta
     2161 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     2221 cctgagcgtg cagtccaaac tttcgaaaga ccccaacgag aagcgcgatc acatggtcct
     2281 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcgg
     2341 taccggaggg agcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     2401 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggtgaggg
     2461 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     2521 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     2581 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acatccagga
     2641 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     2701 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     2761 catcctgggg cacaagctgg agtacaacaa tcatgaccaa ctgaaaagag aaactaaagt
     2821 cctgaagact ctgtcggtga tcatgggtgt gtttgtgtgc tgttggctac ctttcttcat
     2881 cttgaactgc attttgccct tctgtgggtc tggggagacg cagcccttct gcattgattc
     2941 caacaccttt gacgtgtttg tgtggtttgg gtgggctaat tcatccttga accccatcat
     3001 ttatgccttt aatgctgatt ttcggaaggc attttcaacc ctcttaggat gctacagact
     3061 ttgccctgcg acgaataatg ccatagagac ggtgagtatc aataacaatg gggccgcgat
     3121 gttttccagc catcatgagc cacgaggctc catctccaag gagtgcaatc tggtttacct
     3181 gatcccacat gctgtgggct cctctgagga cctgaaaaag gaggaggcag ctggcatcgc
     3241 cagacccttg gagaagctgt ccccagccct atcggtcata ttggactatg acactgacgt
     3301 ctctctggag aagatccaac ccatcacaca aaacggtcag cacccaacct gaaagcttat
     3361 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     3421 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     3481 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     3541 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     3601 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     3661 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     3721 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     3781 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     3841 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     3901 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     3961 ccgtcgaccc gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca
     4021 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     4081 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     4141 tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa
     4201 atgtggtaaa atcgataagg atcttcctag agcatggcta cgtagataag tagcatggcg
     4261 ggttaatcat taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg
     4321 ctcgctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     4381 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     4441 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4501 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4561 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4621 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4681 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4741 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4801 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     4861 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4921 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4981 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     5041 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     5101 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     5161 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     5221 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     5281 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     5341 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     5401 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     5461 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     5521 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     5581 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     5641 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     5701 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     5761 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     5821 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     5881 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5941 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     6001 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     6061 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     6121 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     6181 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     6241 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     6301 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     6361 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     6421 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     6481 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     6541 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     6601 gaaagcgaaa g
//
