LOCUS       sequence-250996-        6614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the red genetically encoded dopamine sensor RdLight1 in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Patriarchi T, Mohebi A, Sun J, Marley A, Liang R, Dong C, Puhger K, 
            Mizuno GO, Davis CM, Wiltgen B, von Zastrow M, Berke JD, Tian L
  TITLE     An expanded palette of dopamine sensors for multiplex imaging in 
            vivo.
  JOURNAL   Nat Methods. 2020 Nov;17(11):1147-1155. doi: 
            10.1038/s41592-020-0936-3. Epub 2020 Sep 7.
  PUBMED    32895537
REFERENCE   2  (bases 1 to 6614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0936-3"; journalName: "Nat Methods"; date: 
            "2020-11"; volume: "17"; issue: "11"; pages: "1147-1155"
COMMENT     Sequence Label: pAAV-CAG-RdLight1
FEATURES             Location/Qualifiers
     source          1..6614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   528..657
                     /label=AAV2 ITR
     primer_bind     1524..1546
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1608..1627
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    3734..4322
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3787..3807)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4205..4216)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4365..4486
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4376..4395)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4430..4449
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(4865..5453)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4945..4964)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5624..6415,6416..6484))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6247..6266
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6485..6589)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg cgcgctcgct
      541 cgctcactga ggccgcccgg gcaaagcccg ggcgtcgggc gacctttggt cgcccggcct
      601 cagtgagcga gcgagcgcgc agagagggag tggccaactc catcactagg ggttccttgt
      661 agttaatgat taacccgcca tgctacttat ctacgtagcc atgctctagg aagagtacca
      721 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      781 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      841 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      901 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      961 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
     1021 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
     1081 gggggggggc gcgcgccrgg sggggsgggg sggggsgggg ggsggggsgg ggsgrggsgg
     1141 agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
     1201 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc
     1261 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
     1321 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
     1381 cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc
     1441 cgggagggcc ctttgtgcgg ggggagcggc tcggggctgt ccgcgggggg acggctgcct
     1501 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
     1561 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
     1621 tattgtgctg tctcatcatt ttggcaaaga attggatcca ccggtgcgcg ccatgaagac
     1681 gatcatcgcc ctgagctaca tcttctgcct ggtgttcgcc gactacaagg acgatgatga
     1741 cgccatgagg actctgaaca cctctgccat ggacgggact gggctggtgg tggagaggga
     1801 cttctctgtt cgtatcctca ctgcctgttt cctgtcgctg ctcatcctgt ccacgctcct
     1861 ggggaacacg ctggtctgtg ctgccgttat caggttccga cacctgcggt ccaaggtgac
     1921 caacttcttt gtcatctcct tggctgtgtc agatctcttg gtggccgtcc tggtcatgcc
     1981 ctggaaggca gtggctgaga ttgctggctt ctggcccttt gggtccttct gtaacatctg
     2041 ggtggccttt gacatcatgt gctccactgc atccatcctc aacctctgtg tgatcagcgt
     2101 ggacaggtat tgggctatct ccagccctga acggtatgag agaaagatga cccccaaggc
     2161 agccttcatc ctgatcagtg tggcatggac cttgtctgta ctcatctcct tcatcccagt
     2221 gcagctcagc tggcacaagg caaaacccac aagcccctct gatggaaatg ccacttccct
     2281 ggctgagacc atagacaact gtgactccag cctcagcagg acatatgcca tctcatcctc
     2341 tgtaatcagc ttttacatcc ctgtggccat catgattgtc acctacacca ggatctacag
     2401 gattgctcag aaacagctct cctcctttca tgtgtcagag cggatgtatc cagaagatgg
     2461 agcgttgaag tcagagatca aaaaaggact gcggttgaag gatggcggac attatgcagc
     2521 tgaggtcaag actacatata aagctaaaaa acccgttcag cttccgggtg cgtatatcgt
     2581 agatattaaa ctcgacattg tttctcacaa cgaggattat acaatcgtcg agcaatgcga
     2641 aagggcagaa ggacgacact ccaccggggg aatggatgag ctttataaag gaggtactgg
     2701 tggcagcctt gtctcaaagg gggaggaaga caatatggca ataattaaag agttcatgag
     2761 attcaaagta cacatggagg gaagcgtcaa tggtcacgag ttcgagatcg aaggggaagg
     2821 ggagggacgc ccttatgagg cctttcagac cgccaaattg aaagtaacaa agggtggacc
     2881 ccttccattt gcgtgggaca tcctctcccc tcagtttatg tatggtagta aggcttatat
     2941 aaagcatccg gctgatatac cagattactt taaactttcc ttcccggaag ggtttcgctg
     3001 ggaacgcgtc atgaatttcg aggatggggg cattatacac gtcaatcaag atagctcctt
     3061 gcaagatggt gtctttatct ataaggtaaa gctgagaggg acaaattttc caccggatgg
     3121 accagtaatg cagaagaaaa ctatggggtg ggaagcacgg aaggacgacc ttaaaagaga
     3181 aactaaagtc ctgaagactc tgtcggtgat catgggtgtg tttgtgtgct gttggctacc
     3241 tttcttcatc ttgaactgca ttttgccctt ctgtgggtct ggggagacgc agcccttctg
     3301 cattgattcc aacacctttg acgtgtttgt gtggtttggg tgggctaatt catccttgaa
     3361 ccccatcatt tatgccttta atgctgattt tcggaaggca ttttcaaccc tcttaggatg
     3421 ctacagactt tgccctgcga cgaataatgc catagagacg gtgagtatca ataacaatgg
     3481 ggccgcgatg ttttccagcc atcatgagcc acgaggctcc atctccaagg agtgcaatct
     3541 ggtttacctg atcccacatg ctgtgggctc ctctgaggac ctgaaaaagg aggaggcagc
     3601 tggcatcgcc agacccttgg agaagctgtc cccagcccta tcggtcatat tggactatga
     3661 cactgacgtc tctctggaga agatccaacc catcacacaa aacggtcagc acccaacctg
     3721 aaagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     3781 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     3841 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     3901 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     3961 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     4021 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     4081 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     4141 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     4201 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     4261 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     4321 gcatcgatac cgtcgacccg ggcggccgct tcgagcagac atgataagat acattgatga
     4381 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     4441 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     4501 cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa
     4561 cctctacaaa tgtggtaaaa tcgataagga tcttcctaga gcatggctac gtagataagt
     4621 agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg gccactccct
     4681 ctctgcgcgc tcgctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     4741 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     4801 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     4861 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     4921 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     4981 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5041 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5101 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5161 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5221 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5281 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     5341 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5401 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     5461 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     5521 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     5581 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     5641 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     5701 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     5761 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     5821 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     5881 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     5941 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     6001 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     6061 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     6121 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     6181 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     6241 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     6301 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     6361 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     6421 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     6481 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     6541 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     6601 gaaaagtgcc acct
//
