LOCUS       sequence-25100-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Noguchi C, Garabedian MV, Malik M, Noguchi E
  TITLE     A vector system for genomic FLAG epitope-tagging in 
            Schizosaccharomyces pombe.
  JOURNAL   Biotechnol J. 2008 Oct;3(9-10):1280-5. doi: 10.1002/biot.200800140.
  PUBMED    18729046
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J."; date: "2008-10"; volume: "3(9-10):1280-5. doi"; pages: " 
            10.1002/biot.200800140"
COMMENT     Sequence Label: pFA6a-his3-P3nmt1-3FLAG
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(59..246)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     167..191
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(270..341)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 gcgagctcgt cgctgcggtc gacggatcta tattaccctg ttatccctag cggatctgcc
       61 ggtagaggtg tggtcaataa gagcgacctc atactatacc tgagaaagca acctgaccta
      121 caggaaagag ttactcaaga ataagaattt tcgttttaaa acctaagagt cactttaaaa
      181 tttgtataca cttatttttt ttataactta tttaataata aaaatcataa atcataagaa
      241 attcgcttat ttagaagtgg cgcgccctat ttatcatcat cgtccttata gtccttatcg
      301 tcgtcatctt tgtaatcctt gtcatcgtcg tccttgtagt ccatattaat taaagacatg
      361 atttaacaaa gcgactataa gtcagaaagt gagaatgaga ttgaaataat taattcaact
      421 tattcaattg atgatatgcc aggattcctc ttcctttata tatcttcaat gtttcgatta
      481 acaggtccat tatccggttt ttagtttcgc tgcacattgc cgaatgacaa tcggattcct
      541 ttaagctttg cttttgccct gttttctctc cccagtaatc gttgggtgac tgaaccatcc
      601 agtagccgag agttacgttt ttcctgattt aataaatatc catatctatt tgtctcgtgg
      661 tgttttacag caactaaccg aaattactat tttgcatctc ncccccttng aagnccncca
      721 taattctgga aacatatnac aggtgttttt tctattacgt ctcatacgca aggtcccntt
      781 accagntttg gaacttgtac ccaccatcca ttttcgattt gtatgaggnc
//
