LOCUS       sequence-25101-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Noguchi C, Garabedian MV, Malik M, Noguchi E
  TITLE     A vector system for genomic FLAG epitope-tagging in 
            Schizosaccharomyces pombe.
  JOURNAL   Biotechnol J. 2008 Oct;3(9-10):1280-5. doi: 10.1002/biot.200800140.
  PUBMED    18729046
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J."; date: "2008-10"; volume: "3(9-10):1280-5. doi"; pages: " 
            10.1002/biot.200800140"
COMMENT     Sequence Label: pFA6a-his3-P41nmt1-3FLAG
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(60..247)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     168..192
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(271..342)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 gcgagctcgt cgctgcaggt cgacggatct atattaccct gttatcccta gcggatctgc
       61 cggtagaggt gtggtcaata agagcgacct catactatac ctgagaaagc aacctgacct
      121 acaggaaaga gttactcaag aataagaatt ttcgttttaa aacctaagag tcactttaaa
      181 atttgtatac acttattttt tttataactt atttaataat aaaaatcata aatcataaga
      241 aattcgctta tttagaagtg gcgcgcccta tttatcatca tcgtccttat agtccttatc
      301 gtcgtcatct ttgtaatcct tgtcatcgtc gtccttgtag tccatattaa ttaaagacat
      361 gatttaacaa agcgactata agtcagaaag tgagaatgag attgaaataa ttaattcaac
      421 ttattcaatt gatgatatgc caggattcct cttcctttat cttcaatgtt tcgattaaca
      481 ggtccattat ccggttttta gtttcgctgc acattgccga atgacaatcg gattccttta
      541 agctttgctt ttgccctgtt ttctctcccc agtaatcgtt gggtgactga accatccagt
      601 agccgagagt tacgtttttc ctgatttaat aaatatccat atctatttgt ctcgtggtgt
      661 tttacagcaa ctaaccgaaa ttactatttt gcatcatcca accataaaga gacaacaata
      721 attctagaaa nntanancag gggtttttnc tntttcgctc ncagcggaag ccccttttta
      781 cggnnttngg aactggnccc cccctccntt ttcgatttgg aaggngncct ttctc
//
