LOCUS       sequence-251047-        1346 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses H2B-mAvicFP1 in mammalian cells (human histone 2B).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Lambert GG, Depernet H, Gotthard G, Schultz DT, Navizet I, Lambert 
            T, Adams SR, Torreblanca-Zanca A, Chu M, Bindels DS, Levesque V, 
            Nero Moffatt J, Salih A, Royant A, Shaner NC
  TITLE     Aequorea's secrets revealed: New fluorescent proteins with unique 
            properties for bioimaging and biosensing.
  JOURNAL   PLoS Biol. 2020 Nov 2;18(11):e3000936. doi: 
            10.1371/journal.pbio.3000936. eCollection 2020 Nov.
  PUBMED    33137097
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2020-11-2"; volume: "18(11):e3000936. doi"; pages: " 
            10.1371/journal.pbio.3000936. eCollection 2020 Nov"
COMMENT     Sequence Label: H2B-mAvicFP1
FEATURES             Location/Qualifiers
     source          1..1346
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             98..475
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      488..497
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgagc
       61 tcaagcttcg aattctgcag tcgacggtac cgccaccatg ccagagccag cgaagtctgc
      121 tcccgccccg aaaaagggct ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa
      181 gaagcgcaag cgcagccgca aggagagcta ttccatctat gtgtacaagg ttctgaagca
      241 ggtccaccct gacaccggca tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa
      301 cgacattttc gagcgcatcg caggtgaggc ttcccgcctg gcgcattaca acaagcgctc
      361 gaccatcacc tccagggaga tccagacggc cgtgcgcctg ctgctgcctg gggagttggc
      421 caagcacgcc gtgtccgagg gtactaaggc catcaccaag tacaccagcg ctaaggatcc
      481 accggtcgcc accatggtga gcaagggcga ggagctgttc aacggtatcg ttcctatcct
      541 gatcgaactg aacggtgacg ttcatggtca taagttctct gttcgtggtg aaggtgaagg
      601 tgacgctggt tctggtaaga tcgaaatcaa gttcgtttgc accaccggta ccctgcctgt
      661 tccttggcct accctggtta ccaccttatg ctacggtgtt cagtgcttca cccgttaccc
      721 tgaacatatg aagcagcatg acttctacaa gtctgctatg cctgacggtt acatccagaa
      781 gcgtaccatc tctttccagg acgacggtca ttacaagacc cgtgctgaag ttaagttcga
      841 aggtgacacc ctggttaacc gtatcgaact gaagggtatc gacttcaagg aagacggtaa
      901 catcctgggt aacaagatgg aatacaacta caactctcat tctgtttacg ttctgtctga
      961 caaggctaac aacggtatca aggttaactt caagatccgt cataacctga agggtgaagg
     1021 tatccagctg gctgaccatg accagcagaa catccctatc ggtgacggtc ctgttctgct
     1081 gcctgactac cattacctgt ctacccagac caagatcacc aaggacccta acgaaaagcg
     1141 tgaccatatg aacctggttg aattcgttac cgcttgcggt atcacccatg gcatggacga
     1201 gctgtacaag taagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg
     1261 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     1321 caattgttgt tgttaactgt ttatgc
//
