LOCUS       sequence-25105-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Noguchi C, Garabedian MV, Malik M, Noguchi E
  TITLE     A vector system for genomic FLAG epitope-tagging in 
            Schizosaccharomyces pombe.
  JOURNAL   Biotechnol J. 2008 Oct;3(9-10):1280-5. doi: 10.1002/biot.200800140.
  PUBMED    18729046
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J."; date: "2008-10"; volume: "3(9-10):1280-5. doi"; pages: " 
            10.1002/biot.200800140"
COMMENT     Sequence Label: pFA6a-5FLAG-bleMX6
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(91..288)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(294..668)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVSDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVDEEQD"
ORIGIN
        1 cgcggccgca tggccactag nnggatctga tatcatcgat gaattcgagc tcgtttaaac
       61 tggatggcgg cgttagtatc gaatcgacag cagtatagcg accagcattc acatacgatt
      121 gacgcatgat attactttct gcgcacttaa cttcgcatct gggcagatga tgtcgaggcg
      181 aaaaaaaata taaatcacgc taacatttga ttaaaataga acaactacaa tataaaaaaa
      241 ctatacaaat gacaagttct tgaaaacaag aatcttttta ttgtcagtac tgatcagtcc
      301 tgctcctcgt ccacgaagtg cacgcagttg ccggccgggt cgcgcagggc gaactcccgc
      361 ccccacggct gctcgccgat ctcggtcatg gccggcccgg aggcgtcccg gaagttcgtg
      421 gacacgacct ccgaccactc ggcgtacagc tcgtccaggc cgcgcaccca cacccaggcc
      481 agggtgttgt ccgacaccac ctggtcctgg accgcgctga tgaacagggt cacgtcgtcc
      541 cggaccacac cggcgaagtc gtcctccacg aagtcccggg agaacccgag ccggtcggtc
      601 cagaactcga ccgctccggc gacgtcgcgc gcggtgagca ccggaacggc actggtcaac
      661 ttggccatgg ttgtttatgt tcggatgtga tgtgagaact gtatcctagc aagattttaa
      721 aaggaagtat atgaaagaag aacctcagtg gcaaatccta ccttttantt ttctctacag
      781 gggcgcggng ngggacaatt caccgcgtct gtgaggggag cgtttcctgc tcngaggtnt
      841 g
//
