LOCUS       sequence-251050-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses LifeAct-mAvicFP1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Lambert GG, Depernet H, Gotthard G, Schultz DT, Navizet I, Lambert 
            T, Adams SR, Torreblanca-Zanca A, Chu M, Bindels DS, Levesque V, 
            Nero Moffatt J, Salih A, Royant A, Shaner NC
  TITLE     Aequorea's secrets revealed: New fluorescent proteins with unique 
            properties for bioimaging and biosensing.
  JOURNAL   PLoS Biol. 2020 Nov 2;18(11):e3000936. doi: 
            10.1371/journal.pbio.3000936. eCollection 2020 Nov.
  PUBMED    33137097
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2020-11-2"; volume: "18(11):e3000936. doi"; pages: " 
            10.1371/journal.pbio.3000936. eCollection 2020 Nov"
COMMENT     Sequence Label: LifeAct-mAvicFP1
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             44..94
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      110..119
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgcc accatgggcg tggccgactt
       61 gatcaagaag ttcgagtcca tctccaagga ggagggggat ccaccggtcg ccaccatggt
      121 gagcaagggc gaggagctgt tcaacggtat cgttcctatc ctgatcgaac tgaacggtga
      181 cgttcatggt cataagttct ctgttcgtgg tgaaggtgaa ggtgacgctg gttctggtaa
      241 gatcgaaatc aagttcgttt gcaccaccgg taccctgcct gttccttggc ctaccctggt
      301 taccacctta tgctacggtg ttcagtgctt cacccgttac cctgaacata tgaagcagca
      361 tgacttctac aagtctgcta tgcctgacgg ttacatccag aagcgtacca tctctttcca
      421 ggacgacggt cattacaaga cccgtgctga agttaagttc gaaggtgaca ccctggttaa
      481 ccgtatcgaa ctgaagggta tcgacttcaa ggaagacggt aacatcctgg gtaacaagat
      541 ggaatacaac tacaactctc attctgttta cgttctgtct gacaaggcta acaacggtat
      601 caaggttaac ttcaagatcc gtcataacct gaagggtgaa ggtatccagc tggctgacca
      661 tgaccagcag aacatcccta tcggtgacgg tcctgttctg ctgcctgact accattacct
      721 gtctacccag accaagatca ccaaggaccc taacgaaaag cgtgaccata tgaacctggt
      781 tgaattcgtt accgcttgcg gtatcaccca tggcatggac gagctgtaca agtaagcggc
      841 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
      901 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
      961 gttat
//
