LOCUS       sequence-251051-        4992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of plant LINC fluorescent fusion protein for cell 
            biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   McKenna JF, Gumber HK, Turpin ZM, Jalovec AM, Kartick AC, Graumann 
            K, Bass HW
  TITLE     Maize (Zea mays L.) Nucleoskeletal Proteins Regulate Nuclear 
            Envelope Remodeling and Function in Stomatal Complex Development and
            Pollen Viability.
  JOURNAL   Front Plant Sci. 2021 Feb 17;12:645218. doi: 
            10.3389/fpls.2021.645218. eCollection 2021.
  PUBMED    33679862
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2021-02-17"; volume: "12:645218. doi"; pages: " 
            10.3389/fpls.2021.645218. eCollection 2021"
COMMENT     Sequence Label: MKAKU41ec
FEATURES             Location/Qualifiers
     source          1..4992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1388..1411
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1415..1441
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(3117..3216)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3233..3252)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3234..3252)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3257..3273)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3257..3273)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3375..3394)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3386..4195
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3453..3472)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4342..4930
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4831..4850
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc atggttagca agggagaaga ggacaatatg gcgataatca aagagttcat
      721 gcggttcaag gttcacatgg agggctcggt caacgggcac gaattcgaga ttgaaggaga
      781 gggcgaagga aggccatacg agggcactca gacagcaaag ttgaaggtca ccaagggcgg
      841 accactccct ttcgcttggg acattctgtc tccacagttc atgtacggct ccaaggccta
      901 tgtgaagcat ccagcagaca tccctgatta tctcaagctg agcttccctg aaggcttcaa
      961 gtgggagaga gttatgaatt ttgaagatgg tggcgtggtt accgtcactc aggactcttc
     1021 actgcaagat ggagagttca tctacaaggt gaagcttcgc ggtactaact ttccatctga
     1081 cggccctgtt atgcagaaga agacaatggg ttgggaagcc tccagcgaga ggatgtatcc
     1141 tgaagatggt gcactgaagg gcgagatcaa gcagagactt aagttgaagg acggaggtca
     1201 ctacgatgct gaggtcaaga ctacatataa ggccaagaag ccagtgcaat tgcctggcgc
     1261 ttacaatgtt aacattaagc tcgacatcac ttcccacaat gaggattaca caatcgttga
     1321 gcagtatgaa agggccgagg gaagacatag cactggcggc atggatgagt tgtacaaggg
     1381 tgcaggcgat tataaggatg acgatgacaa ggcttaccca tatgacgtgc ctgattacgc
     1441 aggagctggt atggcaagcc tcttcggagc taggagacgc aggtcaccag aatatgatgg
     1501 tgaagatgat agatccggtg gcggaagggc aaagagaaga aggctgagcc ctgaggaagc
     1561 tgccgcatct ccagcagagc ctggagctgc cacaggtacc agccatggct ggcttagcgg
     1621 atttgtctct ggtgctaaga gggccatttc ttcagtgctc ctgtccagct ctccagagga
     1681 aactggctct ggagaagacg gagaggtcga ggaagaggat gacgatgtgt acgaagaggg
     1741 catcgatttg aatgagaacg aagacattca cgatatccat ggtgaaattg tcccttattc
     1801 tgagtcaaag ctcgccatcg aacaaatggt tatgaaggag acattctcaa gagacgaatg
     1861 cgatcgcatg gttgagctga ttaagtcaag agtccgcgat tccactccag aaacacacga
     1921 gtacggcaag caggaagaga ttccttccag aaatgcagga atcgctcatg acttcactgg
     1981 tacatgccgc tcccttagca gggatagaaa cttcacagag tctgtgccat tttcatccat
     2041 gcgcatgagg cctggccaca gctctccagg atttcctctt caggcttcac cacaattgtg
     2101 caccgcagct gtgagggaag ccaagaagtg gcttgaagag aagagacaag gtttgggcgt
     2161 taagccagag gacaatggct cttgcaccct caacactgat attttctcat cccgcgacga
     2221 ttcagacaag ggttcccctg ttgatcttgc caagagctat atgaggtctt tgccaccttg
     2281 gcagtcccca ttcctcggac atcaaaagtt tgacacctct ccttcaaagt acagcatcag
     2341 ctctactaag gttaccacta aggaggatta tctctcatcc ttttggacta agctggaaga
     2401 gtctagaatt gctcgcatcg gcagctctgg agactcagcc gtcgcatcca agctgtggaa
     2461 ttacggctcc aacagcagac ttttcgaaaa tgatacctca atcttttccc tgggcactga
     2521 cgagaaggtt ggagatccta ccaagactca caacggcagc gaaaaggtcg ccgcaaccga
     2581 gccacttgga cgctgctctc ttttgattac acctaccgaa gacaggactg atgtgtcata
     2641 taaggttgcc gagggcaatg acgtgtcatc cacaggaatc accaaggaca caaccggtca
     2701 ttcagccgat ggcaaggcac tcacatccga accacacatt ggcgagactc atgttaattc
     2761 tgcatcagag tccatcccta acgatgctgc cccacctact cagtctaaga tgaatggtag
     2821 cacaaagaag tctctcgtca acggcgtgct ggaccaacca aatgctaact caggattgga
     2881 gagctctggt aatgattacc cttcctatac aaactcatcc agcgctatgc cacctgccag
     2941 caccgagctc attggttctg cagctgccgt tatcgacgtc gattctgctg aaaacggacc
     3001 aggtactaag ccagagcagc ctgccaaggg cgcatcaagg gctagcaagt ctaaggttgt
     3061 gccacgggga cagaagaggg tgctgcggtc ggctacacgg ggacgggcta cgtgagaccc
     3121 agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     3181 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     3241 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     3301 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     3361 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
     3421 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     3481 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
     3541 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     3601 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     3661 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc
     3721 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     3781 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
     3841 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     3901 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
     3961 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     4021 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     4081 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     4141 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     4201 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag
     4261 ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     4321 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4381 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4441 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4501 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4561 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4621 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4681 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     4741 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4801 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4861 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4921 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4981 tgctcacatg tt
//
