LOCUS       sequence-251080-        7650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Hughes CM, Rozenblatt-Rosen O, Milne TA, Copeland TD, Levine SS, Lee
            JC, Hayes DN, Shanmugam KS, Bhattacharjee A, Biondi CA, Kay GF, 
            Hayward NK, Hess JL, Meyerson M
  TITLE     Menin associates with a trithorax family histone methyltransferase 
            complex and with the hoxc8 locus.
  JOURNAL   Mol Cell. 2004 Feb 27. 13(4):587-97.
  PUBMED    14992727
REFERENCE   2  (bases 1 to 7650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2004 Feb 27. 13(4):587-97."
COMMENT     Sequence Label: pBABE hygro MEN1 P12L
FEATURES             Location/Qualifiers
     source          1..7650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(451..1242,1243..1311))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1074..1093
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1312..1416)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             1447..1916
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1981..2180
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             2381..2797
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     2727..2749
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     2765..2781
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      2956..2965
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5286..5306)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5291..5620
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5471..5606
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5694..6719
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5694..6719
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     7236..7255
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             join(7517..7650,1..336)
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg
       61 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
      121 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
      181 cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca
      241 ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct
      301 ctgagtgatt gactacccgt cagcgggggt ctttcacatg cagcatgtat caaaattaat
      361 ttggtttttt ttcttaagta tttacattaa atggccatag tgcggccgca aatcaatcta
      421 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
      481 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
      541 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
      601 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
      661 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
      721 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
      781 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
      841 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
      901 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
      961 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     1021 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     1081 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     1141 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     1201 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     1261 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     1321 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     1381 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     1441 tgcagcctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
     1501 ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
     1561 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt
     1621 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
     1681 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
     1741 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg
     1801 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
     1861 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg
     1921 ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc accgggaggc
     1981 aagctggcca gcaacttatc tgtgtctgtc cgattgtcta gtgtctatga ctgattttat
     2041 gcgcctgcgt cggtactagt tagctaacta gctctgtatc tggcggaccc gtggtggaac
     2101 tgacgagttc tgaacacccg gccgcaaccc tgggagacgt cccagggact ttgggggccg
     2161 tttttgtggc ccgacctgag gaagggagtc gatgtggaat ccgaccccgt caggatatgt
     2221 ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc
     2281 ggtttggaac cgaagccgcg cgtcttgtct gctgcagcgc tgcagcatcg ttctgtgttg
     2341 tctctgtctg actgtgtttc tgtatttgtc tgaaaattag ggccagactg ttaccactcc
     2401 cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt
     2461 agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt
     2521 cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt
     2581 cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc
     2641 cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc
     2701 tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc
     2761 ctccctttat ccagccctca ctccttctct aggcgccggc cggatcccag tgtggtggta
     2821 cgtaggaatt cccgggtcga cccacgcgtc cggtgtccgg agccgcggac ctagagatcc
     2881 cagaagccac agcgcagcgg cccggcccgc cactatttcc aggctctgcg gggcaggggc
     2941 cgccgcccac cgcccgccgc catggggctg aaggccgccc agaagacgct gttcctgctg
     3001 cgctccatcg acgacgtggt gcgcctgttt gctgccgagc tgggccgaga ggagccggac
     3061 ctggtgctcc tttccttggt gctgggcttc gtggagcatt ttctggctgt caaccgcgtc
     3121 atccctacca acgttcccga gctcaccttc cagcccagcc ccgcccccga cccgcctggc
     3181 ggcctcacct actttcccgt ggccgacctg tctatcatcg ccgccctcta tgcccgcttc
     3241 accgcccaga tccgaggcgc cgtcgacctg tccctctatc ctcgagaagg gggtgtctcc
     3301 agccgtgagc tggtgaagaa ggtctccgat gtcatatgga acagcctcag ccgctcctac
     3361 ttcaaggatc gggcccacat ccagtccctc ttcagcttca tcacaggcac caaattggac
     3421 agctccggtg tggcctttgc tgtggttggg gcctgccagg ccctgggtct ccgggatgtc
     3481 cacctcgccc tgtctgagga tcatgcctgg gtagtgtttg ggcccaatgg ggagcagaca
     3541 gctgaggtca cctggcacgg caagggcaac gaggaccgca ggggccagac agtcaatgcc
     3601 ggtgtggctg agcggagctg gctgtacctg aaaggatcat acatgcgctg tgaccgcaag
     3661 atggaggtgg cgttcatggt gtgtgccatc aacccttcca ttgacctgca caccgactcg
     3721 ctggagcttc tgcagctgca gcagaagctg ctctggctgc tctatgacct gggacatctg
     3781 gaaaggtacc ccatggcctt agggaacctg gcagatctag aggagctgga gcccacccct
     3841 ggccggccag acccactcac cctctaccac aagggcattg cctcagccaa gacctactat
     3901 cgggatgaac acatctaccc ctacatgtac ctggctggct accactgtcg caaccgcaat
     3961 gtgcgggaag ccctgcaggc ctgggcggac acggccactg tcatccagga ctacaactac
     4021 tgccgggaag acgaggagat ctacaaggag ttctttgaag tagccaatga tgtcatcccc
     4081 aacctgctga aggaggcagc cagcttgctg gaggcgggcg aggagcggcc gggggagcaa
     4141 agccagggca cccagagcca aggttccgcc ctccaggacc ctgagtgctt cgcccacctg
     4201 ctgcgattct acgacggcat ctgcaaatgg gaggagggca gtcccacgcc tgtgctgcac
     4261 gtgggctggg ccacctttct tgtgcagtcc ctaggccgtt ttgagggaca ggtgcggcag
     4321 aaggtgcgca tagtgagccg agaggccgag gcggccgagg ccgaggagcc gtggggcgag
     4381 gaagcccggg aaggccggcg gcggggccca cggcgggagt ccaagccaga ggagcccccg
     4441 ccgcccaaga agccagcact ggacaagggc ctgggcaccg gccagggtgc agtgtcagga
     4501 cccccccgga agcctcctgg gactgtcgct ggcacagccc gaggccctga aggtggcagc
     4561 acggctcagg tgccagcacc cgcagcatca ccaccgccgg agggtccagt gctcactttc
     4621 cagagtgaga agatgaaggg catgaaggag ctgctggtgg ccaccaagat caactcgagc
     4681 gccatcaagc tgcaactcac ggcacagtcg caagtgcaga tgaagaagca gaaagtgtcc
     4741 acccctagtg actacactct gtctttcctc aagcggcagc gcaaaggcct ctgaactact
     4801 ggggacttcg gaccgcttgt ggggacccag gctccgccct tagtccccca actctgagcc
     4861 catgttctgc ccccagccca aaggggacag gcctcacctc tacccaaacc ctaggttccc
     4921 ggtcccgagt acagtctgta tcaaacccac gattttctcc agctcagaac ccagggctct
     4981 gccccagtcg ttagaatata ggtctcttct cccagaatcc cagccggcca atggaaacct
     5041 cacgctgggt cctaattacc agtctttaaa ggcccagccc ctagaaaccc aagctcctcc
     5101 tcggaaccgc tcacctagag ccagaccaac gttactcagg gctcctccca gcttgtagga
     5161 gctgaggttt cacccttaac ccaaggagca caggtcccac ctccagcccg ggagcctagg
     5221 accactcagc ccctaggagt atatttccgc acttcagaat tcgccagcac agtggtcgac
     5281 cctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     5341 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     5401 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     5461 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     5521 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     5581 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttgcca gctggggcgc
     5641 cctctggtaa ggttgggaag ccctgcagat ctgatcccgg ggggcaatga gatatgaaaa
     5701 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     5761 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     5821 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     5881 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     5941 ttagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     6001 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
     6061 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     6121 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     6181 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     6241 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     6301 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     6361 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     6421 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     6481 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     6541 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     6601 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     6661 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataga
     6721 gtagatgccg accgggatct atcgatgcgg cgggtgtggt ggttacgcgt aatctgctgc
     6781 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6841 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     6901 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6961 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7021 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7081 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7141 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7201 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7261 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7321 cggagcctat ggaaaaacgc cagcaacgca tcgataaaat aaaagatttt atttagtctc
     7381 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg
     7441 ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca
     7501 ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     7561 cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
     7621 gtaagcagtt cctgccccgg ctcagggcca
//
