LOCUS       sequence-251092-        1836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses OT-I TCRA linked to TCRB with the P2A self-cleaving 
            peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Aksoy P, Aksoy Bulent Arman, Czech E, Hammerbacher J
  TITLE     Viable and efficient electroporation-based genetic manipulation of 
            unstimulated human T cells
  JOURNAL   bioRxiv
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv"
COMMENT     Sequence Label: pcDNA3.1(+)-OTI-TCRA_p2_TCRB
FEATURES             Location/Qualifiers
     source          1..1836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             832..888
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ggatccatgg acaagatcct gacagcatcg tttttactcc taggccttca cctagctggg
       61 gtgaatggcc agcagcagga gaaacgtgac cagcagcagg tgagacaaag tccccaatct
      121 ctgacagtct gggaaggaga gaccgcaatt ctgaactgca gttatgagga cagcactttt
      181 aactacttcc catggtacca gcagttccct ggggaaggcc ctgcactcct gatatccata
      241 cgttcagtgt ccgataaaaa ggaagatgga cgattcacaa tcttcttcaa taaaagggag
      301 aaaaagctct ccttgcacat cacagactct cagcctggag actcagctac ctacttctgt
      361 gcagcaagtg acaactatca gttgatctgg ggctctggga ccaagctaat tataaagcca
      421 gacatccaga acccagaacc tgctgtgtac cagttaaaag atcctcggtc tcaggacagc
      481 accctctgcc tgttcaccga ctttgactcc caaatcaatg tgccgaaaac catggaatct
      541 ggaacgttca tcactgacaa aactgtgctg gacatgaaag ctatggattc caagagcaat
      601 ggggccattg cctggagcaa ccagacaagc ttcacctgcc aagatatctt caaagagacc
      661 aacgccacct accccagttc agacgttccc tgtgatgcca cgttgactga gaaaagcttt
      721 gaaacagata tgaacctaaa ctttcaaaac ctgtcagtta tgggactccg aatcctcctg
      781 ctgaaagtag ccggatttaa cctgctcatg acgctgaggc tgtggtcctc cgccacgaac
      841 ttctctctgt taaagcaagc aggagacgtg gaagaaaacc ccggtcctat gtctaacact
      901 gtcctcgctg attctgcctg gggcatcacc ctgctatctt gggttactgt ctttctcttg
      961 ggaacaagtt cagcagattc tggggttgtc cagtctccaa gacacataat caaagaaaag
     1021 ggaggaaggt ccgttctgac gtgtattccc atctctggac atagcaatgt ggtctggtac
     1081 cagcagactc tggggaagga attaaagttc cttattcagc attatgaaaa ggtggagaga
     1141 gacaaaggat tcctacccag cagattctca gtccaacagt ttgatgacta tcactctgaa
     1201 atgaacatga gtgccttgga actggaggac tctgctatgt acttctgtgc cagctctcgg
     1261 gccaattatg aacagtactt cggtcccggc accaggctca cggttttaga ggatctgaga
     1321 aatgtgactc cacccaaggt ctccttgttt gagccatcaa aagcagagat tgcaaacaaa
     1381 caaaaggcta ccctcgtgtg cttggccagg ggcttcttcc ctgaccacgt ggagctgagc
     1441 tggtgggtga atggcaagga ggtccacagt ggggtcagca cggaccctca ggcctacaag
     1501 gagagcaatt atagctactg cctgagcagc cgcctgaggg tctctgctac cttctggcac
     1561 aatcctcgaa accacttccg ctgccaagtg cagttccatg ggctttcaga ggaggacaag
     1621 tggccagagg gctcacccaa acctgtcaca cagaacatca gtgcagaggc ctggggccga
     1681 gcagactgtg gaatcacttc agcatcctat catcaggggg ttctgtctgc aaccatcctc
     1741 tatgagatcc tactggggaa ggccacccta tatgctgtgc tggtcagtgg cctggtgctg
     1801 atggccatgg tcaagaaaaa aaattcctaa gaattc
//
