LOCUS       sequence-251096-        1821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses OT-I TCRB linked to TCRA with the P2A self-cleaving 
            peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Aksoy P, Aksoy Bulent Arman, Czech E, Hammerbacher J
  TITLE     Viable and efficient electroporation-based genetic manipulation of 
            unstimulated human T cells
  JOURNAL   bioRxiv
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv"
COMMENT     Sequence Label: pcDNA3.1(+)-OTI-TCRB_p2_TCRA
FEATURES             Location/Qualifiers
     source          1..1821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             940..996
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 atgtctaaca ctgtcctcgc tgattctgcc tggggcatca ccctgctatc ttgggttact
       61 gtctttctct tgggaacaag ttcagcagat tctggggttg tccagtctcc aagacacata
      121 atcaaagaaa agggaggaag gtccgttctg acgtgtattc ccatctctgg acatagcaat
      181 gtggtctggt accagcagac tctggggaag gaattaaagt tccttattca gcattatgaa
      241 aaggtggaga gagacaaagg attcctaccc agcagattct cagtccaaca gtttgatgac
      301 tatcactctg aaatgaacat gagtgccttg gaactggagg actctgctat gtacttctgt
      361 gccagctctc gggccaatta tgaacagtac ttcggtcccg gcaccaggct cacggtttta
      421 gaggatctga gaaatgtgac tccacccaag gtctccttgt ttgagccatc aaaagcagag
      481 attgcaaaca aacaaaaggc taccctcgtg tgcttggcca ggggcttctt ccctgaccac
      541 gtggagctga gctggtgggt gaatggcaag gaggtccaca gtggggtcag cacggaccct
      601 caggcctaca aggagagcaa ttatagctac tgcctgagca gccgcctgag ggtctctgct
      661 accttctggc acaatcctcg aaaccacttc cgctgccaag tgcagttcca tgggctttca
      721 gaggaggaca agtggccaga gggctcaccc aaacctgtca cacagaacat cagtgcagag
      781 gcctggggcc gagcagactg tggaatcact tcagcatcct atcatcaggg ggttctgtct
      841 gcaaccatcc tctatgagat cctactgggg aaggccaccc tatatgctgt gctggtcagt
      901 ggcctggtgc tgatggccat ggtcaagaaa aaaaattccg ccacgaactt ctctctgtta
      961 aagcaagcag gagacgtgga agaaaacccc ggtcctatgg acaagatcct gacagcatcg
     1021 tttttactcc taggccttca cctagctggg gtgaatggcc agcagcagga gaaacgtgac
     1081 cagcagcagg tgagacaaag tccccaatct ctgacagtct gggaaggaga gaccgcaatt
     1141 ctgaactgca gttatgagga cagcactttt aactacttcc catggtacca gcagttccct
     1201 ggggaaggcc ctgcactcct gatatccata cgttcagtgt ccgataaaaa ggaagatgga
     1261 cgattcacaa tcttcttcaa taaaagggag aaaaagctct ccttgcacat cacagactct
     1321 cagcctggag actcagctac ctacttctgt gcagcaagtg acaactatca gttgatctgg
     1381 ggctctggga ccaagctaat tataaagcca gacatccaga acccagaacc tgctgtgtac
     1441 cagttaaaag atcctcggtc tcaggacagc accctctgcc tgttcaccga ctttgactcc
     1501 caaatcaatg tgccgaaaac catggaatct ggaacgttca tcactgacaa aactgtgctg
     1561 gacatgaaag ctatggattc caagagcaat ggggccattg cctggagcaa ccagacaagc
     1621 ttcacctgcc aagatatctt caaagagacc aacgccacct accccagttc agacgttccc
     1681 tgtgatgcca cgttgactga gaaaagcttt gaaacagata tgaacctaaa ctttcaaaac
     1741 ctgtcagtta tgggactccg aatcctcctg ctgaaagtag ccggatttaa cctgctcatg
     1801 acgctgaggc tgtggtcctc c
//
