LOCUS       sequence-251108-        5686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Choi JH, Zhong X, McAlpine W, Liao TC, Zhang D, Fang B, Russell J, 
            Ludwig S, Nair-Gill E, Zhang Z, Wang KW, Misawa T, Zhan X, Choi M, 
            Wang T, Li X, Tang M, Sun Q, Yu L, Murray AR, Moresco EMY, Beutler B
  TITLE     LMBR1L regulates lymphopoiesis through Wnt/beta-catenin signaling.
  JOURNAL   Science. 2019 May 10;364(6440). pii: 364/6440/eaau0812. doi: 
            10.1126/science.aau0812.
  PUBMED    31073040
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2019-05-10"; volume: "364(6440). pii: 364/6440/eaau0812. 
            doi"; pages: " 10.1126/science.aau0812"
COMMENT     Sequence Label: pCMV-N-HA-Gp78
FEATURES             Location/Qualifiers
     source          1..5686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          646..742
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             806..832
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    2799..2933
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2823..2842)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2877..2896
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3044..3060)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3044..3060)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3057..3079)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3068..3084
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3092..3098,3099..3116,3117..3122))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3137..3158
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3275..3292)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3446..4034)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3526..3545)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4205..4996,4997..5065))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4828..4847
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5066..5170)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5238..5256
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5294..5316)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5416..5435
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5629..5651
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5643..5660
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5644..5660
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
      601 agaggatccg gtactagagg aactgaaaaa ccagaaagtt aactggtaag tttagtcttt
      661 ttgtctttta tttcaggtcc cggatccggt ggtggtgcaa atcaaagaac tgctcctcag
      721 tggatgttgc ctttacttct aggcctgtac ggaagtgtta cttctgctct aaaagctgcg
      781 gaattgtacc cgcgggccca ccatgtaccc atacgatgtt ccagattacg ctcttatggc
      841 catggaggcc cgaattatgc cgctgctctt cctcgagcgc ttcccgtggc ccagcctccg
      901 cacctacacg ggcctcagcg gcctggcctt gctgggcacc atcgtcagcg cctaccgcgc
      961 cctcagtcag ccggaggacg gctcgggtga gccggagccg ctaacggccc cactgcagcc
     1021 cgaggcgcgc ctgaccgccg gcggcccccg ggcccgcgac gtggcccagt acctgctgtc
     1081 ggacagcctg ttcgtgtggg ttctggtgaa tactgcttgc tgtgttttga tgttggtggc
     1141 taagcttatc cagtgtattg tgttcggccc tcttcgagtg agtgaaagac agcatctcaa
     1201 agacaagttt tggaatttta ttttctacaa gttcattttc atttttggag tgctgaatgt
     1261 ccagactgtg gaggaagtgg tgatgtggtg cctctggttt gctggacttg tcttcctgca
     1321 tctgatggtt cagctctgca aagacagatt tgaatacctt tccttttctc ccaccactcc
     1381 aatgagcagc catgggagag tcctgtccct gctgattgct atgctgctct cctgctgtgg
     1441 actggcagtc gtctgctgtg tcacaggcta cacacatggc atgcacacct tggctttcat
     1501 ggctgcagag tctcttcttg tgaccgtgag aacagctcat gtgattttaa gatatgtaat
     1561 tcacctctgg gacctcaacc acgaagggac gtgggaagga aagggaacct atgtgtatta
     1621 caccgacttt gtcatggagc tcgctctcct gtccctggac ctcatgcacc acatccacat
     1681 gctgttattt ggcaacatct ggttatctat ggctagcttg gtcatcttta tgcaacttcg
     1741 ttacctgttc catgaggtac agcgtcgaat ccgtcgacac aagaattatt tgcgagtggt
     1801 aggaaacatg gaagccaggt ttgctgttgc aactccagag gaactagctg tcaataatga
     1861 tgactgtgca atctgctggg actccatgca ggctgcaagg aaactgccct gtggacatct
     1921 ttttcacaac tcctgccttc gttcttggct agaacaagac acctcttgtc caacatgcag
     1981 aatgtctctt aatattgctg atggcagtcg tgccagggaa gaccatcaag gagagaacct
     2041 ggacgagaac ttggttcctg tagcagctgc cgaaggcagg cctcgcttga accagcacaa
     2101 tcacttcttc cacttcgatg ggtcccggat tgcaagctgg ctgccaagtt tttcagttga
     2161 agtgatgcac actaccaaca ttcttggcat cacgcaggca agcaactctc agctaaatgc
     2221 catggctcat cagattcagg agatgttccc tcaggtcccg taccaccttg tgctccagga
     2281 cctccagatg acacgctccg tggagataac aacagataac atcttagaag gacggattca
     2341 agtacctttc cccacgcagc ggtcggatag cctgaggcct gcactgaaca gcccagtgga
     2401 gaggcccagc cctgacctgg aggagggaga agcttctgtc cagacggagc gtgtaccact
     2461 ggacctcagt cctcgcctag aggagacctt ggacttcagt gaggtagaac tagagcctat
     2521 tgaggtggaa gactttgagg cccgtggaag ccgcttctcc aagtctgctg atgagagaca
     2581 gcgcatgtta gttcagcgca aggatgacct cctgcagcag gctcgaaagc ggttcttaaa
     2641 caaaagttct gaagatgatg gggcctcaga gagactcctc ccttctgaag gcacatcctc
     2701 tgaccccgtg accctgcgcc gaaggatgct ggcagccgct gctgagcgga gacttcagag
     2761 gcaacggaca acctagctcg aggtaccgcg gccgcgggga tccagacatg ataagataca
     2821 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     2881 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     2941 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttcggatc
     3001 ctctagagtc gatctgcagg catgctagct tggcgtaatc atggtcatag ctgtttcctg
     3061 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     3121 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     3181 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     3241 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3301 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3361 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3421 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3481 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3541 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3601 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3661 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3721 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3781 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3841 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3901 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3961 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     4021 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4081 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4141 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4201 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4261 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4321 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     4381 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     4441 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4501 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4561 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4621 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4681 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4741 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4801 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4861 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4921 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4981 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     5041 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     5101 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     5161 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     5221 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     5281 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     5341 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     5401 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     5461 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     5521 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     5581 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     5641 gttgtaaaac gacggccagt gagttcgagc ttgcatgcct gcaggt
//
