LOCUS       sequence-251118-       10376 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral vector for expression of HA-Tagged E6-AP I (R39H) in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10376)
  AUTHORS   Kuhnle S, Martinez-Noel G, Leclere F, Hayes SD, Harper JW, Howley PM
  TITLE     Angelman syndrome-associated point mutations in the Zn-binding 
            N-terminal (AZUL) domain of UBE3A ubiquitin ligase inhibit binding 
            to the proteasome.
  JOURNAL   J Biol Chem. 2018 Sep 26. pii: RA118.004653. doi: 
            10.1074/jbc.RA118.004653.
  PUBMED    30257870
REFERENCE   2  (bases 1 to 10376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2018 Sep 26. pii: RA118.004653. doi: 
            10.1074/jbc.RA118.004653."
COMMENT     Sequence Label: PHAGE-P CMVt N-HA hE6-AP I (R39H)
FEATURES             Location/Qualifiers
     source          1..10376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2227..2530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2531..2734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2685..2705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2736..2754
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2757..2775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2785..2809
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2896..2905
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2908..2934
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    2950..2974
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5537..5561)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5580..6079
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5615..5638)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6012..6031
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6100..6699
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6100..6119)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6596..6616
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6766..7354
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6819..6839)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7237..7248)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7429..7662
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7781..7799)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7867..7971
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7972..8040,8041..8832)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8190..8209)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9003..9591
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9492..9511
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9745..9762
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10002..10023
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10038..10043,10044..10061,10062..10068)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10076..10092
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10081..10103
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10100..10116
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10100..10116
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10166..10185
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata gagatctccc
     2761 tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg
     2821 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
     2881 agcgtttaaa cgctagccac catggcgtac ccatacgatg ttccagatta cgctgctcga
     2941 ggttatcaaa caagtttgta caaaaaagtt ggcaccatga agcgagcagc tgcaaagcat
     3001 ctaatagaac gctactacca ccagttaact gagggctgtg gaaatgaagc ctgcacgaat
     3061 gagttttgtg cttcctgtcc aacttttctt catatggata ataatgcagc agctattaaa
     3121 gccctcgagc tttataagat taatgcaaaa ctctgtgatc ctcatccctc caagaaagga
     3181 gcaagctcag cttaccttga gaactcgaaa ggtgccccca acaactcctg ctctgagata
     3241 aaaatgaaca agaaaggcgc tagaattgat tttaaagatg tgacttactt aacagaagag
     3301 aaggtatatg aaattcttga attatgtaga gaaagagagg attattcccc tttaatccgt
     3361 gttattggaa gagttttttc tagtgctgag gcattggtac agagcttccg gaaagttaaa
     3421 caacacacca aggaagaact gaaatctctt caagcaaaag atgaagacaa agatgaagat
     3481 gaaaaggaaa aagctgcatg ttctgctgct gctatggaag aagactcaga agcatcttcc
     3541 tcaaggatag gtgatagctc acagggagac aacaatttgc aaaaattagg ccctgatgat
     3601 gtgtctgtgg atattgatgc cattagaagg gtctacacca gattgctctc taatgaaaaa
     3661 attgaaactg cctttctcaa tgcacttgta tatttgtcac ctaacgtgga atgtgacttg
     3721 acgtatcaca atgtatactc tcgagatcct aattatctga atttgttcat tatcgtaatg
     3781 gagaatagaa atctccacag tcctgaatat ctggaaatgg ctttgccatt attttgcaaa
     3841 gcgatgagca agctacccct tgcagcccaa ggaaaactga tcagactgtg gtctaaatac
     3901 aatgcagacc agattcggag aatgatggag acatttcagc aacttattac ttataaagtc
     3961 ataagcaatg aatttaacag tcgaaatcta gtgaatgatg atgatgccat tgttgctgct
     4021 tcgaagtgct tgaaaatggt ttactatgca aatgtagtgg gaggggaagt ggacacaaat
     4081 cacaatgaag aagatgatga agagcccatc cctgagtcca gcgagctgac acttcaggaa
     4141 cttttgggag aagaaagaag aaacaagaaa ggtcctcgag tggaccccct ggaaactgaa
     4201 cttggtgtta aaaccctgga ttgtcgaaaa ccacttatcc cttttgaaga gtttattaat
     4261 gaaccactga atgaggttct agaaatggat aaagattata cttttttcaa agtagaaaca
     4321 gagaacaaat tctcttttat gacatgtccc tttatattga atgctgtcac aaagaatttg
     4381 ggattatatt atgacaatag aattcgcatg tacagtgaac gaagaatcac tgttctctac
     4441 agcttagttc aaggacagca gttgaatcca tatttgagac tcaaagttag acgtgaccat
     4501 atcatagatg atgcacttgt ccggctagag atgatcgcta tggaaaatcc tgcagacttg
     4561 aagaagcagt tgtatgtgga atttgaagga gaacaaggag ttgatgaggg aggtgtttcc
     4621 aaagaatttt ttcagctggt tgtggaggaa atcttcaatc cagatattgg tatgttcaca
     4681 tacgatgaat ctacaaaatt gttttggttt aatccatctt cttttgaaac tgagggtcag
     4741 tttactctga ttggcatagt actgggtctg gctatttaca ataactgtat actggatgta
     4801 cattttccca tggttgtcta caggaagcta atggggaaaa aaggaacttt tcgtgacttg
     4861 ggagactctc acccagttct atatcagagt ttaaaagatt tattggagta tgaagggaat
     4921 gtggaagatg acatgatgat cactttccag atatcacaga cagatctttt tggtaaccca
     4981 atgatgtatg atctaaagga aaatggtgat aaaattccaa ttacaaatga aaacaggaag
     5041 gaatttgtca atctttattc tgactacatt ctcaataaat cagtagaaaa acagttcaag
     5101 gcttttcgga gaggttttca tatggtgacc aatgaatctc ccttaaagta cttattcaga
     5161 ccagaagaaa ttgaattgct tatatgtgga agccggaatc tagatttcca agcactagaa
     5221 gaaactacag aatatgacgg tggctatacc agggactctg ttctgattag ggagttctgg
     5281 gaaatcgttc attcatttac agatgaacag aaaagactct tcttgcagtt tacaacgggc
     5341 acagacagag cacctgtggg aggactagga aaattaaaga tgattatagc caaaaatggc
     5401 ccagacacag aaaggttacc tacatctcat acttgcttta atgtgctttt acttccggaa
     5461 tactcaagca aagaaaaact taaagagaga ttgttgaagg ccatcacgta tgccaaagga
     5521 tttggcatgc tgtaataccc aactttcttg tacaaagtgg ttcgataacg aattctaccg
     5581 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     5641 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     5701 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     5761 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     5821 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     5881 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     5941 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     6001 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     6061 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg
     6121 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     6181 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     6241 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     6301 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     6361 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     6421 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     6481 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     6541 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     6601 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     6661 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag
     6721 cgcccgaccg aaaggagcgc acgaccccat gcatcgatag atcctaatca acctctggat
     6781 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     6841 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     6901 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     6961 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7021 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7081 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7141 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     7201 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7261 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     7321 acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt
     7381 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     7441 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     7501 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7561 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7621 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     7681 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggcccgggt
     7741 taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt
     7801 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     7861 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     7921 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     7981 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     8041 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     8101 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     8161 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     8221 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     8281 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     8341 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     8401 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     8461 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     8521 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     8581 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     8641 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     8701 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     8761 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     8821 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     8881 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     8941 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9001 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     9061 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     9121 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     9181 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     9241 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     9301 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     9361 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     9421 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     9481 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     9541 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     9601 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     9661 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     9721 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     9781 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct
     9841 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     9901 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc gtggcacgac
     9961 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
    10021 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
    10081 agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt cacaaataaa
    10141 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    10201 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
    10261 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
    10321 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt
//
