LOCUS       sequence-25112-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Noguchi C, Garabedian MV, Malik M, Noguchi E
  TITLE     A vector system for genomic FLAG epitope-tagging in 
            Schizosaccharomyces pombe.
  JOURNAL   Biotechnol J. 2008 Oct;3(9-10):1280-5. doi: 10.1002/biot.200800140.
  PUBMED    18729046
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J."; date: "2008-10"; volume: "3(9-10):1280-5. doi"; pages: " 
            10.1002/biot.200800140"
COMMENT     Sequence Label: pFA6a-bleMX6-P3nmt1-3FLAG
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(90..287)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(293..667)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVSDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVDEEQD"
ORIGIN
        1 gnacgggcgc atggcactag tggatctgat atcatcgatg aattcgagct cgtttaaact
       61 ggatggcggc gttagtatcg aatcgacagc agtatagcga ccagcattca catacgattg
      121 acgcatgata ttactttctg cgcacttaac ttcgcatctg ggcagatgat gtcgaggcga
      181 aaaaaaatat aaatcacgct aacatttgat taaaatagaa caactacaat ataaaaaaac
      241 tatacaaatg acaagttctt gaaaacaaga atctttttat tgtcagtact gatcagtcct
      301 gctcctcgtc cacgaagtgc acgcagttgc cggccgggtc gcgcagggcg aactcccgcc
      361 cccacggctg ctcgccgatc tcggtcatgg ccggcccgga ggcgtcccgg aagttcgtgg
      421 acacgacctc cgaccactcg gcgtacagct cgtccaggcc gcgcacccac acccaggcca
      481 gggtgttgtc cgacaccacc tggtcctgga ccgcgctgat gaacagggtc acgtcgtccc
      541 ggaccacacc ggcgaagtcg tcctccacga agtcccggga gaacccgagc cggtcggtcc
      601 agaactcgac cgctccggcg acgtcgcgcg cggtgagcac cggaacggca ctggtcaact
      661 tggccatggt tgtttatgtt cggatgtgat gtgagaactg tatcctacaa ttatttttna
      721 ggggnaccan ttttnaaaaa anaccnccgg nggcaatcct aacnttttat ntttctctac
      781 aggggcgcgg cgtggggaca nttcacgcgt ctgtgaggga nc
//
