LOCUS       sequence-251131-        1437 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of CD63-pHuji for visualization of multivesicular 
            body-plasma membrane fusion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Verweij FJ, Bebelman MP, Jimenez CR, Garcia-Vallejo JJ, Janssen H, 
            Neefjes J, Knol JC, de Goeij-de Haas R, Piersma SR, Baglio SR, 
            Verhage M, Middeldorp JM, Zomer A, van Rheenen J, Coppolino MG, 
            Hurbain I, Raposo G, Smit MJ, Toonen RFG, van Niel G, Pegtel DM
  TITLE     Quantifying exosome secretion from single cells reveals a modulatory
            role for GPCR signaling.
  JOURNAL   J Cell Biol. 2018 Mar 5;217(3):1129-1142. doi: 
            10.1083/jcb.201703206. Epub 2018 Jan 16.
  PUBMED    29339438
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201703206"; journalName: "J Cell Biol"; date: 
            "2018-03-5- 5"; volume: "217"; issue: "3"; pages: "1129-1142"
COMMENT     Sequence Label: pCMV-Sport6-CD63-pHuji
FEATURES             Location/Qualifiers
     source          1..1437
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             115..822
                     /codon_start=1
                     /product="pH-sensitive red fluorescent protein derived from
                     mApple, for imaging of exocytosis and endocytosis (Shen et 
                     al., 2014)"
                     /label=pHuji
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKYRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     528..547
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggcggtgg aaggaggaat gaaatgtgtg aagttcttgc tctacgtcct cctgctggcc
       61 ttttgcgcct gtgcagtggg actgattgcc gtgggtgtcg gggcacagag atctatggtg
      121 agcaagggcg aggagaataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
      181 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      241 tacgaggcct ttcagaccgc taagctgaag gtgaccaagg gtggccccct gcccttcgcc
      301 tgggacatcc tgtcccctca gttcatgtac ggctccaagg tctacattaa gcacccagcc
      361 gacatccccg actacttcaa gctgtccttc cccgagggct tcaggtggga gcgcgtgatg
      421 aacttcgagg acggcggcat tattcacgtt aaccaggact cctccctgca ggacggcgtg
      481 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
      541 aagaagacca tgggctggga ggcctccgag gagcggatgt accccgagga cggcgccctg
      601 aagagcgaga ttaagtatag gctgaagctg aaggacggcg gccactacgc cgccgaggtc
      661 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacat cgtggacatc
      721 aagttggaca tcgtgtccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
      781 gagggccgcc actccaccgg cggcatggac gagctgtaca agagatctct tgtcctgagt
      841 cagaccataa tccagggggc tacccctggc tctctgttgc cagtggtcat catcgcagtg
      901 ggtgtcttcc tcttcctggt ggcttttgtg ggctgctgcg gggcctgcaa ggagaactat
      961 tgtcttatga tcacgtttgc catctttctg tctcttatca tgttggtgga ggtggccgca
     1021 gccattgctg gctatgtgtt tagagataag gtgatgtcag agtttaataa caacttccgg
     1081 cagcagatgg agaattaccc gaaaaacaac cacactgctt cgatcctgga caggatgcag
     1141 gcagatttta agtgctgtgg ggctgctaac tacacagatt gggagaaaat cccttccatg
     1201 tcgaagaacc gagtccccga ctcctgctgc attaatgtta ctgtgggctg tgggattaat
     1261 ttcaacgaga aggcgatcca taaggagggc tgtgtggaga agattggggg ctggctgagg
     1321 aaaaatgtgc tggtggtagc tgcagcagcc cttggaattg cttttgtcga ggttttggga
     1381 attgtctttg cctgctgcct cgtgaagagt atcagaagtg gctacgaggt gatgtag
//
