LOCUS       sequence-251133-        1467 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of CD81-pHluorin for visualization of multivesicular 
            body-plasma membrane fusion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Verweij FJ, Bebelman MP, Jimenez CR, Garcia-Vallejo JJ, Janssen H, 
            Neefjes J, Knol JC, de Goeij-de Haas R, Piersma SR, Baglio SR, 
            Verhage M, Middeldorp JM, Zomer A, van Rheenen J, Coppolino MG, 
            Hurbain I, Raposo G, Smit MJ, Toonen RFG, van Niel G, Pegtel DM
  TITLE     Quantifying exosome secretion from single cells reveals a modulatory
            role for GPCR signaling.
  JOURNAL   J Cell Biol. 2018 Mar 5;217(3):1129-1142. doi: 
            10.1083/jcb.201703206. Epub 2018 Jan 16.
  PUBMED    29339438
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201703206"; journalName: "J Cell Biol"; date: 
            "2018-03-5- 5"; volume: "217"; issue: "3"; pages: "1129-1142"
COMMENT     Sequence Label: pCMV-Sport6-CD81-pHluorin
FEATURES             Location/Qualifiers
     source          1..1467
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      157..166
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             163..879
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="MGSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(196..224)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     819..839
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 atgggagtgg agggctgcac caagtgcatc aagtacctgc tcttcgtctt caatttcgtc
       61 ttctggctgg ctggaggcgt gatcctgggt gtggccctgt ggctccgcca tgacccgcag
      121 accaccaacc tcctgtatct ggagctgaga tctctagcca ccatgggaag taaaggagaa
      181 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
      241 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
      301 tttatttgca ctactggaaa actacctgtt ccttggccaa cacttgtcac tactttaact
      361 tatggtgttc aatgcttttc aagataccca gatcatatga aacggcatga ctttttcaag
      421 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac
      481 tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta
      541 aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga atacaactat
      601 aacgatcacc aggtgtacat catggcagac aaacaaaaga atggaatcaa agctaacttc
      661 aaaattagac acaacattga agatggaggc gttcaactag cagaccatta tcaacaaaat
      721 actccaattg gcgatgggcc cgtcctttta ccagacaacc attacctgtt tacaacttct
      781 actctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca
      841 gctgctggga ttacacatgg catggatgaa ctatacaaaa ccggtggagc tcgaatcaga
      901 tctggagaca agcccgcgcc caacaccttc tatgtaggca tctacatcct catcgctgtg
      961 ggcgctgtca tgatgttcgt tggcttcctg ggctgctacg gggccatcca ggaatcccag
     1021 tgcctgctgg ggacgttctt cacctgcctg gtcatcctgt ttgcctgtga ggtggccgcc
     1081 ggcatctggg gctttgtcaa caaggaccag atcgccaagg atgtgaagca gttctatgac
     1141 caggccctac agcaggccgt ggtggatgat gacgccaaca acgccaaggc tgtggtgaag
     1201 accttccacg agacgcttga ctgctgtggc tccagcacac tgactgcttt gaccacctca
     1261 gtgctcaaga acaatttgtg tccctcgggc agcaacatca tcagcaacct cttcaaggag
     1321 gactgccacc agaagatcga tgacctcttc tccgggaagc tgtacctcat cggcattgct
     1381 gccatcgtgg tcgctgtgat catgatcttc gagatgatcc tgagcatggt gctgtgctgt
     1441 ggcatccgga acagctccgt gtactag
//
