LOCUS       sequence-251135-        1431 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of CD81-pHuji for visualization of multivesicular 
            body-plasma membrane fusion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Verweij FJ, Bebelman MP, Jimenez CR, Garcia-Vallejo JJ, Janssen H, 
            Neefjes J, Knol JC, de Goeij-de Haas R, Piersma SR, Baglio SR, 
            Verhage M, Middeldorp JM, Zomer A, van Rheenen J, Coppolino MG, 
            Hurbain I, Raposo G, Smit MJ, Toonen RFG, van Niel G, Pegtel DM
  TITLE     Quantifying exosome secretion from single cells reveals a modulatory
            role for GPCR signaling.
  JOURNAL   J Cell Biol. 2018 Mar 5;217(3):1129-1142. doi: 
            10.1083/jcb.201703206. Epub 2018 Jan 16.
  PUBMED    29339438
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201703206"; journalName: "J Cell Biol"; date: 
            "2018-03-5- 5"; volume: "217"; issue: "3"; pages: "1129-1142"
COMMENT     Sequence Label: pCMV-Sport6-CD81-pHuji
FEATURES             Location/Qualifiers
     source          1..1431
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             154..861
                     /codon_start=1
                     /product="pH-sensitive red fluorescent protein derived from
                     mApple, for imaging of exocytosis and endocytosis (Shen et 
                     al., 2014)"
                     /label=pHuji
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKYRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     567..586
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgggagtgg agggctgcac caagtgcatc aagtacctgc tcttcgtctt caatttcgtc
       61 ttctggctgg ctggaggcgt gatcctgggt gtggccctgt ggctccgcca tgacccgcag
      121 accaccaacc tcctgtatct ggagctgaga tctatggtga gcaagggcga ggagaataac
      181 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      241 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgaggcctt tcagaccgct
      301 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      361 ttcatgtacg gctccaaggt ctacattaag cacccagccg acatccccga ctacttcaag
      421 ctgtccttcc ccgagggctt caggtgggag cgcgtgatga acttcgagga cggcggcatt
      481 attcacgtta accaggactc ctccctgcag gacggcgtgt tcatctacaa ggtgaagctg
      541 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      601 gcctccgagg agcggatgta ccccgaggac ggcgccctga agagcgagat taagtatagg
      661 ctgaagctga aggacggcgg ccactacgcc gccgaggtca agaccaccta caaggccaag
      721 aagcccgtgc agctgcccgg cgcctacatc gtggacatca agttggacat cgtgtcccac
      781 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      841 ggcatggacg agctgtacaa gagatctgga gacaagcccg cgcccaacac cttctatgta
      901 ggcatctaca tcctcatcgc tgtgggcgct gtcatgatgt tcgttggctt cctgggctgc
      961 tacggggcca tccaggaatc ccagtgcctg ctggggacgt tcttcacctg cctggtcatc
     1021 ctgtttgcct gtgaggtggc cgccggcatc tggggctttg tcaacaagga ccagatcgcc
     1081 aaggatgtga agcagttcta tgaccaggcc ctacagcagg ccgtggtgga tgatgacgcc
     1141 aacaacgcca aggctgtggt gaagaccttc cacgagacgc ttgactgctg tggctccagc
     1201 acactgactg ctttgaccac ctcagtgctc aagaacaatt tgtgtccctc gggcagcaac
     1261 atcatcagca acctcttcaa ggaggactgc caccagaaga tcgatgacct cttctccggg
     1321 aagctgtacc tcatcggcat tgctgccatc gtggtcgctg tgatcatgat cttcgagatg
     1381 atcctgagca tggtgctgtg ctgtggcatc cggaacagct ccgtgtacta g
//
