LOCUS       sequence-251137-        1443 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of CD9-pHluorin for visualization of multivesicular 
            body-plasma membrane fusion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Verweij FJ, Bebelman MP, Jimenez CR, Garcia-Vallejo JJ, Janssen H, 
            Neefjes J, Knol JC, de Goeij-de Haas R, Piersma SR, Baglio SR, 
            Verhage M, Middeldorp JM, Zomer A, van Rheenen J, Coppolino MG, 
            Hurbain I, Raposo G, Smit MJ, Toonen RFG, van Niel G, Pegtel DM
  TITLE     Quantifying exosome secretion from single cells reveals a modulatory
            role for GPCR signaling.
  JOURNAL   J Cell Biol. 2018 Mar 5;217(3):1129-1142. doi: 
            10.1083/jcb.201703206. Epub 2018 Jan 16.
  PUBMED    29339438
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201703206"; journalName: "J Cell Biol"; date: 
            "2018-03-5- 5"; volume: "217"; issue: "3"; pages: "1129-1142"
COMMENT     Sequence Label: pCMV-Sport6-CD9-pHluorin
FEATURES             Location/Qualifiers
     source          1..1443
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      160..169
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             166..882
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="MGSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(199..227)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     822..842
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 atgccggtca aaggaggcac caagtgcatc aaatacctgc tgttcggatt taacttcatc
       61 ttctggcttg ccgggattgc tgtccttgcc attggactat ggctccgatt cgactctcag
      121 accaagagca tcttcgagca agaaactaat agatctctag ccaccatggg aagtaaagga
      181 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
      241 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
      301 aaatttattt gcactactgg aaaactacct gttccttggc caacacttgt cactacttta
      361 acttatggtg ttcaatgctt ttcaagatac ccagatcata tgaaacggca tgactttttc
      421 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
      481 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
      541 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
      601 tataacgatc accaggtgta catcatggca gacaaacaaa agaatggaat caaagctaac
      661 ttcaaaatta gacacaacat tgaagatgga ggcgttcaac tagcagacca ttatcaacaa
      721 aatactccaa ttggcgatgg gcccgtcctt ttaccagaca accattacct gtttacaact
      781 tctactcttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
      841 acagctgctg ggattacaca tggcatggat gaactataca aaaccggtgg agctcgaatc
      901 agatctaata ataattccag cttctacaca ggagtctata ttctgatcgg agccggcgcc
      961 ctcatgatgc tggtgggctt cctgggctgc tgcggggctg tgcaggagtc ccagtgcatg
     1021 ctgggactgt tcttcggctt cctcttggtg atattcgcca ttgaaatagc tgcggccatc
     1081 tggggatatt cccacaagga tgaggtgatt aaggaagtcc aggagtttta caaggacacc
     1141 tacaacaagc tgaaaaccaa ggatgagccc cagcgggaaa cgctgaaagc catccactat
     1201 gcgttgaact gctgtggttt ggctgggggc gtggaacagt ttatctcaga catctgcccc
     1261 aagaaggacg tactcgaaac cttcaccgtg aagtcctgtc ctgatgccat caaagaggtc
     1321 ttcgacaata aattccacat catcggcgca gtgggcatcg gcattgccgt ggtcatgata
     1381 tttggcatga tcttcagtat gatcttgtgc tgtgctatcc gcaggaaccg cgagatggtc
     1441 tag
//
